APOBEC3B (BC053859) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APOBEC1L; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B; ARCD3; ARP4; bK150C2.2; cytidine deaminase; DJ742C19.2; FLJ21201; OTTHUMP00000028684; phorbolin-1-related; phorbolin 2; phorbolin 3; PHRBNL
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC053859 edited GACTAGTTCTAGATCGCGATCTAGAACTAGTAGCTTCAAAAAAAGAGCGGGACAGGGACA AGCGTATCTAAGAGGCTGAACATGAATCCACAGATCAGAAATCCGATGGAGCGGATGTAT CGAGACACATTCTACGACAACTTTGAAAACGAACCCATCCTCTATGGTCGGAGCTACACT TGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAATCTCCTTTGGGACACAGGG GTCTTTCGAGGCCAGGTGTATTTCGAGCCTCAGTACCACGCAGAAATGTGCTTCCTCTCT TGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGATCACCTGGTTTGTATCC TGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGTCTGAGCACCCCAAT GTCACCCTGACCATCTCCGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGG GCGCTCTGCAGGCTGAGTCAGGCAGGAGCCCGCGTGAAGATCATGGACTATGAAGAATTT GCATACTGCTGGGAAAACTTTGTGTACAATGAAGGTCAGCAATTCATGCCTTGGTACAAA TTCGATGAAAATTATGCATTCCTGCACCGCACGCTAAAGGAGATTCTCAGGCTAAGAATC TTCTCTGTGGCTTTTACGGCCGCCATGCGGAGCTGCGCTTCTTGGACCTGGTTCCTTCTT TGCAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTCATCTCCTGGAGCCCCTGCT TCTCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACACACGTGAGAC TGCGCATCTTCGCTGCCCGCATCTATGATTATGACCCCCTATATAAGGAGGCGCTGCAAA TGCTGCGGGATGCTGGGGCCCAAGTCTCCATCATGACCTACGATGAGTTTGAGTACTGCT GGGACACCTTTGTGTACCGCCAGGGATGTCCCTTCCAGCCCTGGGATGGACTAGAGGAGC ACAGCCAAGCCCTGAGTGGGAGGCTGCGGGCCATTCTCCAGAATCAGGGAAACTGAAGGA TGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGATCTTC TTCCAAGAAATGCAAACAGACCGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAA AGCAATGTGCTCCTGATCAAGTAGATTTTTTAAAAATCAGAGTCAATTAATTTTAATTGA AAATTTCTCTTATGTTCCAAGTGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAG TTTTCAATGTATTAATGAAGTGATTAATTGGCTCCATATTTAGACTAATAAAACATTAAG AATCTTCCATAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC053859 unedited GCGTCAAAATTGTATACGACTCATATAGGCGGCCGCGNAATCAGATCTGGTACCGGTCCG GAATTCCCGGGATATCGTCGACCCACGCGTCCGGACTAGTTCTAGATCGCGATCTAGAAC TAGTAGCTTCAAAAAAAGAGCGGGACAGGGACAAGCGTATCTAAGAGGCTGAACATGAAT CCACAGATCAGAAATCCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAA AACGAACCCATCCTCTATGGTCGGAGCTACACTTGGCTGTGCTATGAAGTGAAAATAAAG AGGGGCCGCTCAAATCTCCTTTGGGACACAGGGGTCTTTCGAGGCCAGGTGTATTTCGAG CCTCAGTACCACGCAGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCT TACAAGTGTTTCCAGATCACCTGGTTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCG AAGCTGGCCGAATTCCTGTCTGAGCACCCCAATGTCACCCTGACCATCTCCGCCGCCCGC CTCTACTACTACTGGGAAAGAAGATTACCGAAGGCGCTCTGCAGGCTGAGTCAGGCAGGA GCCCGCGTGAAGATCATGGACTATGAAGAATTTGCATACTGCTGGGAAAACTTTGTGTAC AATGANAGGTCAGCATTCATGCCTTGGTACANATTCGATGAANAATATGCATNTCTGCAC CGCACGCTAAAAGAGATTCTCANGCTAAGAATCTTCTCTGTGGCTTTTACGGCCGCCATG CGGAGCTGCGCTTTCTGGACTGGGTTACTTCTTGCAGTTGGACCGGGCCAGATCTACAGG GTCACTGGNTTCATCTCCTGGAGCCCCTGCTTCTACTGGGGCTGTGCGA
Restriction Sites	NotI-NotI
ACCN	BC053859
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC053859.1, AAH53859.1
RefSeq Size	1408 bp
Locus ID	9582
Gene Summary	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

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