CPSF30 (CPSF4) (BC050738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cleavage-polyadenylation specificity factor, 30kD; cleavage and polyadenylation specific factor 4, 30kDa; CPSF30; NAR; NEB1; no arches-like zinc finger protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC050738 edited GGCGAAGCGAAGGAGGAGTGTGTGCGGCGGGGCCGGCGGCGGGTAAAGGCGAGAAGGCTG CAGGAGACCGAGGGGGAGCCGGGCCGGTGGGGCCGCCGCCGCCGCCATGCAGGAAATCAT CGCCAGCGTGGACCACATCAAGTTTGACTTGGAGATCGCGGTGGAGCAGCAGCTGGGGGC GCAGCCGCTGCCCTTCCCCGGCATGGACAAGTCGGGCGCTGCTGTCTGTGAATTCTTTTT GAAAGCTGCCTGCGGCAAAGGGGGCATGTGTCCGTTTCGCCACATCAGTGGTGAGAAGAC AGTTGTGTGCAAACACTGGCTGCGTGGCCTATGCAAGAAAGGGGACCAGTGTGAGTTCCT GCATGAGTATGACATGACCAAGATGCCCGAGTGCTACTTCTACTCCAAGTTCGGGGAGTG CAGCAACAAGGAATGTCCCTTCCTGCACATCGACCCCGAGTCCAAGATCAAGGACTGTCC TTGGTATGACCGCGGCTTCTGCAAGCACGGTCCCCTCTGCAGGCACCGGCACACACGGAG AGTCATCTGTGTGAATTACCTCGTGGGATTCTGCCCGGAGGGGCCCTCGTGTAAATTCAT GCACCCTCGATTTGAACTGCCCATGGGAACCACCGAGCAGCCCCCACTGCCACAGCAGAC ACAGCCTCCAGCAAAGAGAACCCCGCAGGTCATCGGGGTCATGCAGAGTCAAAACAGCAG CGCGGGCAACCGGGGACCCCGGCCACTGGAGCAGGTCACCTGTTACAAGTGTGGCGAGAA AGGACACTACGCCAACAGATGCACCAAAGGGCACTTGGCCTTTCTCAGTGGACAGTGACA GCAGCTGGAGCCAGCTCCGAGCAGCCCGGGGGCCCCGCTGTTGGGAGTGTGCATTTAACT GTTTCATGCGCTTGTTGGCGCGACTGTGGCTCGAGCTGGCCCGCAGACACGTGGGTTTCA TCACTCTGAGGGGCCACGTCTGTTAGTTTCCTATCATTTTGCCGTAGTATTTTTTGAAAA AGGGACATGTGTCCTGTGGGTCCCTGCAGTCGACATCATGTTTGGCTGGGCATCGATGCC TCCTTTCTGGGACTCCCGGCACAACTCCCTGCCCTGCTGAATCCTAAAGCTGTGCCTATA TCTGTGATTTGAATGAGGGAGCCCTTTGGGGCAAATTCAGGTGCCCCCATTGCCTCAGGC TGGCCCTGGTCCCAGGTGGCAGCGGTTGAGGAGGGGTACAGGGCTCTCAAGCCTGAGGTT TTCTTCTCTGGGCTTAATTTTCTCTTGGGGTACGTGCCTGACAGTGTTTAAGGTGTCCGT TGAACTGGAGTTGCAGACTTTTAAATAGATGACCCCTTCAGATCATCTGTGCCTACCTCC TGCCCATCAGGCGTCTACACTGTCACTCAGACACCTGTGGCATGTGGAGGAGACTGCCCT GTCCTGAGCCTGGAAAATGTGAAACTGTCTCCTGCAACCTGCTGGGCATGTGGGCCTGGC TGTGTTCAATTGCAAGAACAATTTTTATGAAATGGATTAAAGCTTGTTTTTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC050738 unedited NGGAGTTTGAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGG TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGCGAAGCGAAGGAGGAGTGTGT GCGGCGGGGCCGGCGGCGGGTAAAGGCGAGAAGGCTGCAGGAGACCGAGGGGGAGCCGGG CCGGTGGGGCCGCCGCCGCCGCCATGCAGGAAATCATCGCCAGCGTGGACCACATCAAGT TTGACTTGGAGATCGCGGTGGAGCAGCAGGTGGGGGCGCAGCCGCTGCCCTTCCCCGGCA TGGACAAGTCGGGCGCTGCTGTCTGTGAATTCTTTTTGAAAGCTGCCTGCGGCAAAGGGG GCATGTGTCCGTTTCGCCACATCAGTGGTGAGAAGACAGTTGTGTGCAAACACTGGCTGC GTGGCCTATGCAAGAAAGGGGACCAGTGTGAGTTCCTGCATGAGTATGACATGACCAAGA TGCCCGAGTGCTACTTCTACTCCAAGTTCGGGGAGTGCAGCAACAAGGAATGTCCCTTCC TGCACATCGACCCCGAGTCCAAGATCAAGGACTGTCCTTGGTATGACCGCGGCTTCTGCA AGCACGGTCCCCTCTGCAGGCACCGGCACACACGGAGAGTCATCTGTGTGAATTACCTCG TGGGATTCTGCCCGGAGGGGCCCTCGTGTAAATTCATGCACCCTCGATTTGAACTGCCCA TGGGAACCACCGAGCAGCCCCCACTGCCACAGCAGACACAGCCTCCAGCAAAGAGAACCC CGCCAGTCATCGGGGTCATGCAGAGTCAAACAGCAGCGCGGGCAACCGGGGACCCCGGCC ACTGGAGCAGGTCACCTGTTACAAGTGTGGC
Restriction Sites	NotI-NotI
ACCN	BC050738
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC050738.1, AAH50738.1
RefSeq Size	1640 bp
Locus ID	10898
Gene Summary	Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

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