A RAF (ARAF) (BC009028) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009028 edited CCCACGCGTCCGAGGCGGTGGTGGTGGTAGCGGTGTGACAGGAGGCCTATGGCAGCTGCC CAGTCCTACCTCAGCCCATCTTGAAAACAATCTTAGGCAACATGGAACCACCACGAGGCC CTCCTGTTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGTACCTGCCTA ACAAGCAACGCACAGTGGTGACTGTCCGGGAAGGCATGAGTGTCTATGACTCTTTGGACA AGGCCCTGAAGGTGCGGGGTCTCAACCAGGACTGCTGTGTGGTCTACAGGCTCATCAAAG GAAGAAAGACAGTCACTGCCTGGGACACAGCCATTGCGCCTCTGGATGGTGAGGAGCTCA TTGTGGAGGTCCTAGAAGATGTGCCACTGACCATGCACAATTTTGTACGGAAGACATTCT TCAGCCTGGCCTTCTGTGACTTCTGCCTTAAGTTTCTGTTCCATGGATTCCGCTGCCAGA CCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTCCCCACCGTCTGCGTTGACA TGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATCTGTCTGGAGGCTCCAGGC AGCAGGAGGCTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGGT AGGGATGTCGTAGATGAAAGGCCAGGGGGCCAGTGAAAGAAGGATTGGAGGACCCTGAGA GATTATACTTTCGTTTATTTGTTTACGTGATAAATACTATTGAGTACCTACAAGTGTTGA ATATGGGCATGGGATTGTGGGATCATTGGTAAGGCACCCAGTCACTCCAGGTTTCATTTT TTTCATCTGGGAAGAGGGGATGAGATTTTCCCTAGTGGGTTCTGAAGAGGATTAAGGGTT GAGATATTTGCAATGCTTAAAGCAAGATCTGATACAGAGTAAATAACAAGTGTTATTCAT TACAAGTGTATATATAAAATCATCTAGTTTGTTCATTTCTTCCAGTATAACCACACCCCA GTCTTTTCTCTTTGAAATAAATGTTACTGTATCAGAAAGTACTTTTATTTCACTTATGTT GGATATGATAGCATATAGTTTTGCATAATATATAAAAAGTAAGGTTAATGTATTATTATT TGTAGTTATGAGTGCATGTGTGTTGATTTACCTGTGAATTTAATCTCAGGATGGTGAAAG TAACAGAAGAATGTTTTTCTGTAAGGTATAATTCATGGGAGGTGGAGGCAAGACGATCAA TAGTTCAAGGCTACCCTCCACTAAGTAGCAAGTTCAAGGCCAGCCTGGGCTACATGAGAC TGTCTTAAAACAGTAAAAAAGGTGTTTTGTAACGTGATATATGTATAATCGTTATATGAT GTGTGTAATGTGTATTACATAATATAATATGTGTATTGAAAACGTTGCCATTTTAATATA GGTAATGCTCATTATTATAAAATAAATAATATATTTGAAAAATACATGTCATTATGATAT GTAATAAAAAGTCATATCTTACCCTTTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009028 unedited ACTTTGAATTTGTATACGACTTATATGGGCGGCCGCGNATTCAGATCTGGTACCGGTCCG GAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGAGGCGGTGGTGGTGG TAGCGGTGTGACAGGAGGCCTATGGCAGCTGCCCAGTCCTACCTCAGCCCATCTTGAAAA CAATCTTAGGCAACATGGAACCACCACGAGGCCCTCCTGTTAGTGGGGCTGAGCCATCTC GGGCAGTTGGCACTGTCAAAGTGTACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCC GGGAAGGCATGAGTGTCTATGACTCTTTGGACAAGGCCCTGAAGGTGCGGGGTCTCAACC AGGACTGCTGTGTGGTCTACAGGCTCATCAAAGGAAGAAAGACAGTCACTGCCTGGGACA CAGCCATTGCGCCTCTGGATGGTGAGGAGCTCATTGTGGAGGTCCTAGAAGATGTGCCAC TGACCATGCACAATTTTGTACGGAAGACATTCTTCAGCCTGGCCTTCTGTGACTTCTGCC TTAAGTTTCTGTTCCATGGATTCCGCTGCCAGACCTGTGGCTACAAGTTCCACCAGCATT GTTCCTCCAAGGTCCCCACCGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACC ACAGCATCCAGGATCTGTCTGGAGGCTCCAGGCAGCAGGAGGCTCCCTCAAATCTCTCTG TGAATGAGCTGCTAACCCCCCCAGGTCCCANNGTAGGATGTCGTANATGAAAGCCAGGGG GCCAGTGAAAGAAGGATTGGAGGACCCTGAGAGATATACCTTTCGTTATTGGTTACGTGA TAATACTATGAGTACCTACAGTGTTGATATGGGCATGGGATGGTGGATCATGGTAAAGCA CCCGTCATCCCAGTTTCATTTTTTTATA
Restriction Sites	Please inquire
ACCN	BC009028
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009028.1, AAH09028.1
RefSeq Size	1542 bp
Locus ID	369
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
Gene Summary	This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

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