SPEM1 (BC033882) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C17orf83
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033882 edited GGGCCCCGGTGGTCCTAGGGACCCCATGGCCATGGTTGAGCGGCCGAGGCCCGAGTGGGC CTCGTATCACAACTGCAACAGCAACAGCTGCCAGGACCTGGGCAACTCTGTCCTGTTGCT GCTGGGCCTCATCATCTGCATTAACATTAGCATCAATATAGTGACCCTGCTCTGGAGCCG ATTCCGTGGTGTCTTATACCAAGTGTTCCATGATACCATTTGTGAGAAAGAAGCTCCCAA GTCATCATTACTCAGAAAGCAGACCCAGCCCCCTAAGAAGCAGAGTTCTCCTGCAGTCCA TCTTCGGTGCACCATGGACCCTGTGATGATGACTGTGTCCCCGCCCCCAGCTCACCGCCA TCGCCGTCGAGGCTCTCCCACACGCTGTGCTCACTGCCCAGTAGCTTGGGCTCCTGACAC TGATGACGAGAAGCCTCATCAGTACCCAGCCATCTGCTCCTACCACTGGGATGTCCCTGA GGACTGGGAAGGCTTCCAACACACTCAGGGGACCTGGGTTCCCTGGTCTCAGGATGCCCC AGAGTCCCCTCCCCAGACCATCCGCTTCCAGCCTACCGTAGAGGAAAGGCCCCTCAAAAC AGGCATATGGTCCGAGCTGGGCCTAAGGGCCTATGTGTATCCTGTGAACCCCCCACCTCC CAGCCCTGAGGCTCCTAGCCACAAGAACGGTGGGGAGGGGGCGGTGCCAGAGGCAGAGGC GGCTCAGTACCAGCCTGTCCCAGCTCCCACCCTGGGCCCAGCAGTCATCCCTGAATTTTC CCGGCACCGCTCCTCAGGCCGAATAGTGTATGATGCCCGGGACATGAGACGGCGGCTTCG GGAACTGACCCGGGAGGTGGAGGCCCTGTCCGGCTGCTACCCCCTAGCCTCTGGATCCAG CACTGCCGAGGAGACAAGCAAGAATTGGGTGTACCGTTCCCTAACTGGGAGGTGACTGGA AAAAAATAAAAAGGAGAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033882 unedited TTTAGGATTTGTAAACGACTTACTATAGGCGGCCGCGNAATCAAATCTGGTACCGGTCCG GAATTCCCGGGATAGGGCCCCGGTGGTCCTAGGGACCCCATGGCCATGGTTGAGCGGCCG AGGCCCGAGTGGGCCTCGTATCACAACTGCAACAGCAACAGCTGCCAGGACCTGGGCAAC TCTGTCCTGTTGCTGCTGGGCCTCATCATCTGCATTAACATTAGCATCAATATAGTGACC CTGCTCTGGAGCCGATTCCGTGGTGTCTTATACCAAGTGTTCCATGATACCATTTGTGAG AAAGAAGCTCCCAAGTCATCATTACTCAGAAAGCAGACCCAGCCCCCTAAGAAGCAGAGT TCTCCTGCAGTCCATCTTCGGTGCACCATGGACCCTGTGATGATGACTGTGTCCCCGCCC CCAGCTCACCGCCATCGCCGTCGAGGCTCTCCCACACGCTGTGCTCACTGCCCAGTAGCT TGGGCTCCTGACACTGATGACGAGAAGCCTCATCAGTACCCAGCCATCTGCTCCTACCAC TGGGATGTCCCTGAGGACTGGGAAGGCTTCCAACACACTCAGGGGACCTGGGTTCCCTGG TCTCAGGATGCCCCAGAGTCCCCTCCCCAGACCATCCGCTTCCAGCCTACCGTAGAGGAA AGGCCCCTCANAACANGCATATGGTCCGAGCTGGGCCTAANGGCCTATGTGTATCCTGTG AACCCCCCACCTCCCAGCCCCTGAGCTGCTAGCCACAGAACGGTGGGGGAGGGGGCCGTG CCANNAGCANAGGCGGCTCANTACCAGCCTGTCCCAGCTCCCACNTGGGCCCAGCAGTCA TCCCTGAATTTTTCCCGCACCGCTCCTCAGCCGAATAGTGTATGATGCCCCGGACTGANA CGGCGGCTCGGGACTGACCGGGAGT
Restriction Sites	NotI-NotI
ACCN	BC033882
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033882.1, AAH33882.1
RefSeq Size	1009 bp
Locus ID	374768
Protein Families	Transmembrane
Gene Summary	Required for proper cytoplasm removal during spermatogenesis.[UniProtKB/Swiss-Prot Function]

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