LSM8 (BC002742) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae); MAK31-like protein; U6 snRNA-associated Sm-like protein LSm8; YJR022W
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002742 edited GCACCGCTGCGTTACCCGGAACCGCCGGGCCGAACAGCATGACGTCCGCTTTGGAGAACT ACATCAACCGAACTGTTGCCGTTATTACATCAGATGGGAGAATGATTGTGGGAACACTGA AAGGTTTTGACCAGACCATTAATTTGATTTTGGATGAAAGCCATGAACGAGTATTCAGCT CTTCACAGGGGGTAGAACAAGTGGTACTAGGATTATACATTGTAAGAGGTGACAACGTTG CAGTCATTGGAGAAATCGATGAAGAAACAGATTCTGCGCTTGATTTGGGGAATATTCGAG CAGAACCTTTAAATTCTGTAGCACACTGAGGAAAAACTACATACTTGGACATCTGTAAAT CTTTGTACAGAAACTGATTATTCTGAGGATGATATATGGAGTTTTTATGAATGTGTCACT GGATTTTGACTCCTTATTGATTCATTGTAATATGTAAATTAAAATATTTCTACATTTTAT TGAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002742 unedited AGTTCAGATTTGTATACCATTATATAGGCGGCCGCGATTCGCACCAGGCACCGCTGCGTT ACCCGGGAACCGCCGGGGGCGGACAGCATGACGTCCGCTTTGGAGAACTACATCAACCGA ACTGTTGCCGTTATTACATCAGATGGGAGAATGATTGTGGGAACACTGAAGGTTTTGACC AGACCATTAATTTGATTTTGGATGAAAGCCATGAACGAGTATTCAGCTCTTCACAGGGGG TAGAACAAGTGGTACTAGGATTATACATTGTAAGAGGTGACAACGTTGCAGTCATTGGAG AAATCGATGAAGAAACAGATTCTGCGCTTGATTTGGGGAATATTCGAGCAGAACCTTTAA ATTCTGTAGCACACTGAGGAAAAACTACATACTTGGACATCTGTAAATCTTTGTACAGAA ACTGATTATTCTGAGGATGATATATGGAGTTTTTATGAATGTGTCACTGGATTTTGACTC CTTATTGATTCATTGTAATATGTAAATTAAAATATTTCTACATTTTATTGAAAAAAAAAA AAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGG GTGGGCATCCCTGTGACCCCTNCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAG TGCCCACCAGCCCTGGTCTAATAAAATTAAGTGGCATCATTTTTGTCTGACTAGGTGTGC TTCTATAATATTATGGGGGGGAGGGGGGTGNGATTGGAACAAGGGGCAATTTGGGGAAAC CAACTGTAGGGGCCTGGGGGGTTATTGGAAACAAACTTGGGTGCCGTGGGCCAATTTTGG CTCATGGAATCTCCGCCCCTGGGTTAAGGGAATTTCCTGGCTACCCTCCCAGTTGTGGGG ATCCGGGCAGGTTGAGCCGGTAACCTAATTTT
Restriction Sites	Please inquire
ACCN	BC002742
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002742.2, AAH02742.1
RefSeq Size	500 bp
Locus ID	51691
Protein Pathways	RNA degradation, Spliceosome
Gene Summary	This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus. [provided by RefSeq, Jan 2010]

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