TPRKB (BC029492) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGI-121; PRPK (p53-related protein kinase)-binding protein; TP53RK binding protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029492 edited GTTTCGGAGCGGGAGCTCTTCCGAGACGCACTGGGGGCCGGATGAATCCTGCTTATCTGT GAAATGCAGTTAACACATCAGCTGGACCTATTTCCCGAATGCAGGGTAACCCTTCTGTTA TTTAAAGATGTAAAAAATGCGGGAGACTTGAGAAGAAAGGCCATGGAAGGCACCATCGAT GGATCACTGATAAATCCTACAGTGATTGTTGATCCATTTCAGATACTTGTGGCAGCAAAC AAAGCAGTTCACCTCTACAAACTGGGAAAAATGAAGACAAGAACTCTATCTACTGAAATT ATTTTCAACCTTTCCCCAAATAACAATATTTCAGAGGCTTTGAAAAAATTTGGTATCTCA GCAAATGACACTTCAATTCTAATTGTTTACATTGAAGAGGGAGAAAAACAAATAAATCAA GAATACCTAATATCTCAAGTAGAAGGTCATCAGGTTTCTCTGAAAAATCTTCCTGAAATA ATGAATATTACAGAAGTCAAAAAGATATATAAACTCTCTTCACAAGAAGAAAGTATTGGG ACATTATTGGATGCTATCATTTGTAGAATGTCAACAAAAGATGTTTTATGAAATGTCAGA AATATTAACAAAAATTCTCAGCATTAAAGAAAACATTGATTTCCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029492 unedited TATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCTCGACGCGGGTTTCGGAGCGG GAGCTCTTCCGAGACGCACTGGGGGCCGGATGAATCCTGCTTATCTGTGAAATGCAGTTA ACACATCAGCTGGACCTATTTCCCGAATGCAGGGTAACCCTTCTGTTATTTAAAGATGTA AAAAATGCGGGAGACTTGAGAAGAAAGGCCATGGAAGGCACCATCGATGGATCACTGATA AATCCTACAGTGATTGTTGATCCATTTCAGATACTTGTGGCAGCAAACAAAGCAGTTCAC CTCTACAAACTGGGAAAAATGAAGACAAGAACTCTATCTACTGAAATTATTTTCAACCTT TCCCCAAATAACAATATTTCAGAGGCTTTGAAAAAATTTGGTATCTCAGCAAATGACACT TCAATTCTAATTGTTTACATTGAAGAGGGAGAAAAACAAATAAATCAAGAATACCTAATA TCTCAAGTAGAAGGTCATCAGGTTTCTCTGAAAAATCTTCCTGAAATAATGAATATTACA GAAGTCAAAAAGATATATAAACTCTCTTTCACAGAAGAAAGTATTGGGACATTATTGGAT GCTATCATTTGTAGAATGTCAACAAAAGATGTTTTATGAAATGTCAGAAATATTAACAAA ATTCTCAGCATTAAAGAAACATTGATTTCCAAAAAAAAAAAAAAACTCTCCAGCGCTGGA TCCGGCCATAAGGGCCTGATCCTTCGAGGGGGGCCCGGTCCCGAATTAAGCTTGTCCACT CTAATTGGGCCGCGGTCATACTGTTTCTGAACGATCCCGGGGCATCCCTGGGACCCTCCC AGGGCTTTCCTGGCCCTGGAGTTGCCACTCAGTGCCACCAGCTGGTCTATAAAATAAGTG CACATTTGTCTGACTAGTGTCTTCTATATATATGGGTGAGG
Restriction Sites	Please inquire
ACCN	BC029492
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC029492.1, AAH29492.1
RefSeq Size	660 bp
Locus ID	51002
Gene Summary	Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed:22912744, PubMed:28805828).[UniProtKB/Swiss-Prot Function]

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