AP2M1 (BC014030) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AP50; CLAPM1; mu2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014030 edited GGGCAGACGAGCAGGGGGCGGGCGGACATCTTGGGATCCGGAGAGTGGCCGGGCCGGCAG AGCAGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTG CCGCCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAG TCTACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATG CCCGGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTA AGCGGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCT TCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGG AAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTG GCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCA AGAGTCAGCATCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGA TTGGCTGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGG AGAGTGTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCGGGCC GGGTGGTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAATGACA AGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGGAAGC AATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTTGACT CTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTATCGCA CAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGACGCA CCAAACTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCTCAGA AGATCGAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGCATGA AGGGGAAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGCATGG CAGGCATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGACAAGA AGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCCTCTGGCC TCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGATGTCA TCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAGCTGCCAC TAGGCAGCTAGCCCACCTCCCCAGCCACCCTCCTCCACAGGTCCAGGTGCCGCTCCCTCC CCCACCACACATCAGTGTCTCCTCCCTCCTGCTTTGCTGCCTTCCCTTTGCACCAGCCCG AGTCTAGGTCTGGGCCAAGCACATTACAAGTGGGACCGGTGGAGCAGCCCCTGGGCTCCC TGGGCAGGGGAGTTCTGAGGCTCCTGCTCTCCCATCCACCTGTCTGTCCTGGCCTAATGC CAGGCTCTGAGTTCTGTGACCAAAGCCAGGTGGGTTCCCTTTCCTTCCCACCCCTGTGGC CACAGCTCTGGAGTGGGAGGGTTGGTTGCCCCTCACCTCAGAGCTCCCCCAAAGGCCAGT AATGGATCCCCGGCCTCAGTCCCTACTCTGCTTTGGGATAGTGTGAGCTTCATTTTGTAC ACGTGTGACTTCGTCCAGTTACAAACCCAATAAACTCTGTAGAGTGGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014030 unedited GGGTTTAGATTTGTATACGACTTACTATAGGCGGCCGCGNATTCANATCTGGTACCGGTC CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGGGCCGGCAGAGC AGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCG CCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCT ACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCC GGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGC GGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCG AATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAA ACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCT ACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCAAGA GTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGC GGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGA ACCTGCTCATGTCCCCACAAGGGCAAGTGCTGAGTGCCCATGTGTCGGGCCGGGTGGTGA TGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTGGGGATGAATGACAAGATTGTTA TTGAAAAACAGGGCAAGGCACCGCTGGAGAAACAAGCAAGACGGGAAGCAATCAATGCCA TTGGT
Restriction Sites	Please inquire
ACCN	BC014030
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC014030.1, AAH14030.1
RefSeq Size	1939 bp
Locus ID	1173
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Huntington's disease
Gene Summary	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

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