RNF35 (TRIM40) (BC060785) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNF35
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC060785 edited ATAAGAACCACTCATCTGCTGGCCATCTCTCCAGGTCTGGAGCCAGAGAAGATGATCTGA AATTGTAGCAGGAGAAATTGAGGTGTATGAAAAATAATTTCAAGTTTAACAAACACAAGG AAACCGCAGGGTCCATGTCAAAGCTGATGAGCTATTTCTGAAACTCGTGCAGAATTGTGG TTTGTGTGGTCTATGTCACGGCACCCTTGAGGGAGAGTGGGCAATTGCCTGAACTTGGAG GCTGTGTCCTGTCCCCAGGCTGCTCCAGGGCTGCCTCCTTCCGACTGGGCCTTCTTATCT GGGACTGTTGAGGGCAACAGGCCTTCCGAAGACCAGTGAAGAAGGAGGCCCTGCAAACAG GAGGCTGACAGGGTAGGAACGAGGCCATGATCCCTTTGCAGAAGGACAACCAGGAGGAGG GTGTCTGCCCCATCTGCCAGGAGAGCCTGAAGGAGGCCGTGAGCACCAACTGCGGACATC TCTTCTGTCGAGTGTGCCTGACACAGCATGTGGAGAAGGCCTCAGCCTCTGGGGTCTTCT GCTGCCCCCTCTGCCGGAAGCCCTGTTCTGAGGAGGTGCTAGGGACAGGCTATATCTGCC CCAACCACCAGAAGAGGGTGTGCAGGTTCTGTGAGGAGAGCAGACTTCTTCTATGTGTGG AATGCCTGGTGTCCCCTGAACACATGTCTCATCATGAACTGACCATTGAAAATGCCCTCA GCCACTACAAGGAACGACTCAATCGCCGGAGCAGGAAGCTCAGAAAGGACATTGCAGAAC TTCAGCGGCTCAAGGCTCAGCAGGAGAAGAAACTGCAGGCTCTGCAGTTTCAGGTAGACC ACGGGAACCACAGGCTGGAGGCTGGGCCGGAGAGCCAGCACCAAACCAGGGAACAGCTGG GTGCCCTCCCTCAGCAGTGGCTGGGCCAGCTGGAGCACATGCCAGCAGAAGCGGCCAGAA TCCTTGACATCTCCAGGGCAGTAACACAGCTCAGAAGCCTGGTCATTGATCTGGAAAGGA CGGCCAAGGAATTAGACACCAACACACTGAAGAATGCTGGTGACTTACTGAACAGGAGTG CTCCACAGAAATTAGAGGTTATTTATCCCCAGTTGGAGAAAGGAGTCAGTGAATTGCTTC TTCAGCCCCCTCAGAAGCTCTGACCTGTTCATCCCTGGGACACCTCACTTCAGGCTCACC TCAGCCTCCTCTCTCTCCTTCCTCCAACCTGTCCAGGCCCCCACTGGGTCTACCCAGTGC ATCTTCGGGCCTGCCAGCTCCTGAACATGTCACCATTTCTTCATGTCCACAGTCATCACC TGATGCCTGACCCTCTGACTCTTGGACGATAGCCAGCCTCCTTCCAGGACAGGCTCATGC TTGGGGCTGCCACTGTGGAGGTCGGGGCCCATGGTCTCCAGGAGCATTTGTGAAATCTCC ATTTTGCCTGTAAACTGATGGTAGTGCCCATCTCTCACAATCTCATTCAAATAGGATCCT CCAGGCCTCTGAATGGCCCGAGCTCATCAGCAGTGACACCACCTCACATGTGGAGCCCAG CTGAGTTCCTGCAGTACTTGTTGTCTGTACCACTCACCTGGCACTTATTTATTATTGTTG TGGAAGACAGACTCAAAGACAGCCTCCCTTCGTGATCCTCACCTCTTGGAATTCATGCCC TTGTTTGGTCCCCTCCCCTTGAGTGTAAGTGGGATCTGTGACTTGCTTCTAATGAATGGA ATAAGGCAAAGGTGATAGGGTGTCACTCTGGCAACTGTGTTGCATTGTATAGAACTCCTC CTTGCTGGCCCACCCTTTTAGAGCCCCTCCTAGGAGCCAAGAGCAGCTTCCAGCCAACAA CAAGCAGAGGCCCTCAGTCTTGTGGCTGCAAGAACCTGAATTCTGCCAACAACCCGAGTG AGCTTGGAAGCAGATTCCTCCCCAACTGAGCCGGATAAGAACCTAGTCCAGCCAACACCT TGATTATAGTCTTGTGAGTACCTAAGCTGAGGACCCAGTGAAGCTGTGCCAAAATTTCCC ACCCACAGAAACAGTGTGACAATAAATGTGTGTGTGTTTTTTTGTTAAAAAATAAAAAAA AAAAAAAA
Restriction Sites	NotI-NotI
ACCN	BC060785
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060785.1, AAH60785.1
RefSeq Size	2108 bp
Locus ID	135644
Gene Summary	This gene encodes a member of the tripartite motif (TRIM) protein family. The encoded protein may play a role as a negative regulator against inflammation and carcinogenesis in the gastrointestinal tract. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014]

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