G0 Protein alpha (GNAO1) (BC030027) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp686O0962; G-ALPHA-o; GNAO; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha activating polypeptide O
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC030027 edited TCTAAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGGGCTGGAG AATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGCTTCTCCG GAGAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCCCTGGCAG CCATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGAAAGGCTG ACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTCTCTGCAG AGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGCTTCAACC GGTCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGA TTGGGGCCGCCGACTACCAGCCCACCGAGCAGGACATCCTCCGAACCAGGGTCAAAACCA CTGGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTTGACGTCG GAGGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACGGCCATCA TTTTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACCACGAACC GCATGCACGAGTCTCTCATGCTCTTCGACTCCATCTGTAACAACAAGTTCTTCATCGATA CCTCCATCATTCTCTTCCTCAACAAGAAAGATCTCTTTGGCGAGAAGATCAAGAAGTCAC CTTTGACCATCTGCTTTCCTGAATACACAGGCCCCAATACCTATGAAGACGCAGCCGCCT ACATCCAAGCACAATTTGAAAGCAAAAACCGCTCACCCAACAAAGAAATATATTGTCACA TGACTTGTGCCACAGACACGAATAACATCCAGGTGGTGTTCGACGCCGTCACCGACATCA TCATTGCCAACAACCTCCGGGGCTGCGGCTTGTACTGACCTCTTGTCCTGTATAGCAACC TATTTGACTGCTTCACGGACTCTTTGCTGTTGACGTTGATCTCCTGGTAGCATGACCTTT TGGCCTTTGTAAGACACACAGCCTTTCTGTATCAAGCCCCTGTCTAACCTACGACCCCAG AGTGACTGACGGCTGTGTATTTCTGTAGAATGCTGTAGAATCCGGTTTTAGTTGAGTCTT CACATTTAGAATTTGAAAGGAAAAACAGAACATTTCTCATGTGCTTTGTAGCTTAAAAAA AGGAAAACTCACCATTTCATCCATATTTCTTTTTCTTGCAAAACAAACATACCCATCTGC ACCGACGCCTGCCCCCGTCCCACCTCGCGGCGCAGCCCCTGGAGCTCCTGCTGGGGGGCA GGGCCCATCTGCCGCCTGGGGCTCCGGTGCGGTGCACTGGTCCGAGGAGTGTGCTCAGGC CGGGCTTTCCAGAAGGCCACAGGCCACTGCAAACCCTGCTGCCTGCCGGCCGCCCAGACA CCACAGACGCCACCTGGCGGCCCTAAGGCACTCCCGCCACACGCTCACTCCAGGTTCGTA TAGAAAAAGCACAGCTCTCACTGGCCAGTAGCTGCCAAAAAGAACACCCATCGTATAAGG AAATCCAGCCCCATAGAAAACAATCTGCCCACCGACCCCCTTTCTAAGGATAGCCTTTGT AGGGGTTTTTTGGTTCCTTTTTTTTTTTTTTCAGTTTTTGTTTTTACTTTTGTCCCGCCG AGTACTATGTATTACCAGCCCCCCACCCCACAGATCAGTTTTCCTAGTCTTTGGGAAACG GGTTGTGTTTTTTTTCCTTTGCAGTGTTGGATTTCCGGTCCCGGCTGACACCTCCCTTCG TGTGTGCGCCCCTCCCCACCCCTGCCTTTGTTTCGGTGCCCTGTGTCCGCTCTCTCATGT GGGGATGTTTGTGCTGCAGACAAAAAGAACAAAAAAAAATTTTTAAATACCACAAGATGG AAAAAAAAACAAAAAAATTTAAAAAGATGGAATGTAGTGTTTACATTAAAGCCGCCGTTT TCATGACCAATCCTATGTCATTTCTACTTTGACTAATACCCAAACCCTAAACATCTTCAC GGTTTCACTTTTAAGACTTAATCTTTGCTGAAGAAGACCAGTCACAGCCATTTCAAATAA AGAGGAGGCAAAAAGACAAAACACACAAAAAATTAAAAAAAAAAACTTAAAAAAAAAAGG AAAAAAGAAAAAAAAGCCCACGGGTCTTTGTAAGCCTTTCTATTCCCAATCGGTGAGGTT TCTGTGATATCTTACACACTGCCAGTCTACTTCTCTGACTAATGGAAAATTCCTGTGTAG CTCAATAAAAGAGAATGTTTGTCTGCAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC030027 unedited NGGGGGTCAGATTTCTATACGACTCATATAGGCGGCCGCATAACTTCGTATAGCATACAT TATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGTCTAA GAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGGGCGTGGGAGAAT CAGGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGCTTCTCCGGA GAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCCCTGGCAGCC ATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGAAAGGCTGAC GCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTCTCTGCAGAG CTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGCTTCAACCGG TCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGATT GGGGCCGCCGACTACCAGCCCACCGAGCAGGACATCCTCCGAACCAGGGTCAAAACCACT GGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTTGACGTCGGA GGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACGGCCATCATT TTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACCACCAACCGC ATGCACGAGTCTCTCATGCTCTTCGACTCCATCTGTGACAACAAGTGTCTTCATCGATAC CTCCATCATTCTCTTCCTCAACAAGAAAGATCTCTTTGGCGAGAAGATCAAAN
Restriction Sites	NotI-NotI
ACCN	BC030027
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030027.2, AAH30027.2
RefSeq Size	2380 bp
Locus ID	2775
Protein Families	Druggable Genome
Protein Pathways	Long-term depression, Melanogenesis
Gene Summary	The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.