GCP4 (TUBGCP4) (BC009870) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	76P; FLJ14797; gamma tubulin ring complex protein (76p gene); GCP4; tubulin, gamma complex associated protein 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009870 edited GGACCCCGTTGAGCTGCCGAACTTCCGGGACTCCCCCGCGACCCCTTCCCAGCTTCCCGT CCGCTCCGCCGCAGCGATTGTCTCGGTGGGTTGATTCGGCACAAACCGCCCGACCCAGGG GCCGGTGCGCGTGTGGAAGGGGAAGCACTCCCCTCGTGGTCGCCTGGAGGTGCGCTGGAG GAGGGGGTGACATAACCAGGGACTCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTCT TGGCTCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTGGCCTGCAGG TATCGCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCC GGCTCGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTGC AACAGCAGGATCACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGC GGGCCTTCTGCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATT TGGAACAAGAGTTCCTGGGTGATCCCCATCTCTCCATATCACATGTCAACTACTTCCTAG ACCAGTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAAA AGATTCATGGTTGTCAAATCCTGGAAACAGTCTACAAACACAGCTGTGGGGGGTTGCCTC CTGTTCGAAGTGCACTGGAAAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAGC TCTCAGCCTGGATGCTCCATGGACTCCTCTTGGACCAGCATGAAGAATTCTTTATCAAAC AGGGGCCATCTTCTGGTAATGTCAGTGCCCAGCCAGAAGAGGACGAGGAGGATCTGGGCA TTGGGGGACTGACAGGAAAACAACTGAGAGAACTGCAGGACTTGCGCCTGATTGAGGAAG AGAACATGCTGGCACCATCTCTGAAGCAGTTTTCCCTACGAGTGGAGATTTTGCCATCCT ACATTCCAGTGAGGGTTGCTGAAAAAATCCTATTTGTTGGAGAATCTGTCCAGATGTTTG AGAATCAAAATGTGAACCTGACTAGAAAAGGATCCATTTTGAAAAACCAGGAAGACACTT TTGCTGCAGAGCTGCACCGTCTCAAGCAGCAGCCACTCTTCAGCTTGGTGGACTTTGAAC AGGTGGTGGATCGCATTCGCAGCACTGTGGCTGAGCATCTCTGGAAGTTGATGGTAGAAG AATCCGATTTACTGGGTCAGCTGAAGATCATTAAAGACTTTTACCTTCTGGGACGTGGAG AACTGTTTCAGGCCTTCATTGACACAGCTCAACACATGTTGAAAACACCACCCACTGCAG TAACTGAGCATGATGTGAATGTGGCCTTTCAACAGTCAGCACACAAGGTATTGCTAGATG ATGACAACCTTCTCCCTCTGTTGCACTTGACAATCGAGTATCACGGAAAGGAGCACAAAG ATGCTACTCAGGCAAGAGAAGGGCCTTCTCGGGAAACTTCTCCCCGGGAAGCCCCTGCAT CTGGCTGGGCAGCCCTAGGTCTTTCCTACAAAGTACAGTGGCCACTACATATTCTCTTCA CCCCAGCTGTCCTGGAAAAGTACAATGTTGTTTTTAAGTACTTACTGAGTGTGCGCCGGG TGCAAGCTGAGCTGCAGCACTGCTGGGCCCTACAAATGCAGCGCAAGCACCTCAAGTCGA ACCAGACTGATGCAATCAAGTGGCGCCTAAGAAATCACATGGCATTTTTGGTGGATAATC TTCAGTACTATCTCCAGGTAGATGTGTTGGAGTCTCAGTTCTCCCAGCTGCTTCATCAGA TCAATTCTACCCGAGACTTTGAAAGCATCCGATTGGCTCATGACCACTTCCTGAGCAATT TGCTGGCTCAATCCTTTATCCTATTGAAACCTGTGTTTCACTGCCTGAATGAAATCCTAG ATCTCTGTCACAGTTTTTGTTCGCTGGTCAGTCAGAACCTAGGCCCACTGGATGAGCGTG GAGCCGCCCAGCTGAGCATTCTCGTGAAGGGCTTTAGCCGCCAGTCTTCACTCCTGTTCA AGATTCTCTCCAGTGTTCGGAATCATCAGATCAACTCAGATTTGGCTCAACTACTGTTAC GACTAGATTATAACAAATACTATACCCAGGCTGGTGGAACTCTGGGCAGTTTCGGGATGT GAAAATTTCTGGCTCATAAATTGAAATAACAGCCACGTTCCCAAGGTTGTAACAGAAGAT TCAAAACATCCCATTCTAGCCACACACAAATAAATATCTGCGGCTTAGTGAAAAAAAAAA AAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009870 unedited GTTCAGGATATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAC CCCGTTGAGCTGCCGAACTTCCGGGACTCCCCCGCGACCCCTTCCCAGCTTCCCGTCCGC TCCGCCGCAGCGATTGTCTCGGTGGGTTGATTCGGCACAAACCGCCCGACCCAGGGGCCG GTGCGCGTGTGGAAGGGGAAGCACTCCCCTCGTGGTCGCCTGGAGGTGCGCTGGAGGAGG GGGTGACATAACCAGGGACTCGAGGTCCGCCGTGGGAATGATCCACGAACTGCTCTTGGC TCTGAGCGGGTACCCTGGGTCCATTTTCACCTGGAACAAGCGGAGTGGCCTGCAGGTATC GCAGGACTTCCCTTTCCTCCACCCCAGTGAGACCAGTGTCCTGAATCGACTCTGCCGGCT CGGCACAGACTATATTCGCTTCACTGAGTTCATTGAACAGTACACGGGCCATGTGCAACA GCAGGATCACCATCCATCTCAACAGGGCCAAGGTGGGTTACATGGAATCTACCTGCGGGC CTTCTGCACAGGGCTGGATTCTGTTTTGCAGCCTTATCGCCAAGCACTGCTTGATTTGGA ACAAGAGTTCCTGGGTGATCCCCATCTCTCCATATCACATGTCAACTACTTCCTAGACCA GTTCCAGCTTCTTTTTCCCTCTGTGATGGTTGTAGTAGAACAAATTAAAAGTCAAAAGAT TCATGGTTGTCAAATCCTGGGAACAGTCTACAAACACAGCTGTGGGGGGGTTGCCTCCTG TTCGAAGTGCACTGGANAAAATCCTGGCCGTTTGTCATGGGGTCATGTATAAACAGCTCT CAGCCTGGATGCTCCATGGACTNCTCTTGGACCAGCATGAGAATTCTTATCAAACAGGGG CATCTTTCTGTATGTCANTGCCCANCCANAAAGGACAGGAGATCTGGGCTT
Restriction Sites	Please inquire
ACCN	BC009870
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009870.2, AAH09870.1
RefSeq Size	2353 bp
Locus ID	27229
Gene Summary	This gene encodes a component of the gamma-tubulin ring complex, which is required for microtubule nucleation. In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin. Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores. An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin. Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

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