SCAMP4 (BC062598) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ33847; FLJ90105; SCAMP-4; secretory carrier membrane protein 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC062598 edited GACTTGGCGAAGCGCTGCGCTCGCGCCCGGATCCCTCAGGCGGCTGCAGGCTTCAGCCTG CGCTGGTTGGTGAAACAGAGATGTCAGAAAAGGAGAACAACTTCCCGCCACTGCCCAAGT TCATCCCTGTGAAGCCCTGCTTCTACCAGAACTTCTCCGACGAGATCCCAGTGGAGCACC AGGTCCTGGTGAAGAGGATCTACCGGCTGTGGATGTTTTACTGCGCCACCCTCGGCGTCA ACCTCATTGCCTGCCTGGCCTGGTGGATCGGCGGAGGCTCGGGGACCAACTTCGGCCTGG CCTTCGTGTGGCTGCTCCTGTTCACGCCTTGCGGCTACGTGTGCTGGTTCCGGCCTGTCT ACAAGGCCTTCCGAGCCGACAGCTCCTTTAATTTCATGGCGTTTTTCTTCATCTTCGGAG CCCAGTTTGTCCTGACCGTCATCCAGGCGATTGGCTTCTCCGGCTGGGGCGCGTGCGGCT GGCTGTCGGCAATTGGATTCTTCCAGTACAGCCCGGGCGCTGCCGTGGTCATGCTGCTTC CAGCCATCATGTTCTCCGTGTCGGCTGCCATGATGGCCATCGCGATCATGAAGGTGCACA GGATCTACCGAGGGGCTGGCGGAAGCTTCCAGAAGGCACAGACGGAGTGGAACACGGGCA CTTGGCGGAACCCACCGTCGAGGGAGGCCCAGTACAACAACTTCTCAGGCAACAGCCTGC CCGAGTACCCCACTGTGCCCAGCTACCCGGGCAGTGGCCAGTGGCCTTAGAGGGAGCCTG CCCTGCCCCCACCGCCCACCACCTCCTCCCCTTCATTCCTGCTGCTACCCCTGGTCCCGA GGGCTGGGAGTACCTGGGGCCCCATCCCCCCAGCTGGGATGGTGGAAGCCGGTGGTGGCC ACGGACCGCCCCCCTCCTGCCAGGGCCACAGAACCCGTGTTCATCTCATCCGAGAGCGGA GTTCCTCACAAGCACTCCCCAGCAGCCCTTGGCCTCTGCCGTCCACAGGACGCCCTCTTG CTCCCGGAAACGTGTGGTCACCCGCCGTCCACTGCACGGCTGGTACGGCCTTGTCTTCAG GTCTCGAGGCCTGACTCCGGGGGACAGGTGGCAGCAGGTCGGCCGCCCTCCCGTCCTCCC AGAGCTGCTGGCGCTGAGGTCAGAGCGGGTCTGATGGGGAGCTCCGTCTCACCGGCCACC CGCCGTCACCATGGCAGATGCCCTTGGCCGGAACTAATAAGAGGCGTCGGGGCCAGCTTC CGGTCCCCTGCAGTGATAGAGGGCTTGGTGCCTAGCTGAGTCCTCGCTGTCCCCGCCATC CCCTGATCTGTGCGGCTCCAGCCTCGCCCCCTCCCCACGTGCACCATACCTGGGGAGTTC CTGGTCCAGGGTATCCTGGGGCCACCCTCCCTGCCTCCAAAACAGGGATCCCTGGCAGGC TGTCTTTCCACGCCCCTGAGTTCAGAGTCGGGGACCCAGGCCAGGTCGGGAGCACAGCCG CTCCCCAAACCCAGCAAACCGGCAGAGAGCCGGTTTCCCAGCAGCCGGAGCCCTGCAGGA GAGGCCTTTGTGTTTTGTTTTGTTTTGTTTTTTCTCTTTTGAGACAGAGTTTCACTCTGT CGCCCAGGCTGGAGTGCAGTGGTGTGATCTCGGCTCACTGCAACCTCTGCCTCCCGTGTT CAAGCAGTTCTCCTGCCTCAGCCTCCCAAATAGCTGGGATTACAGTTGCCTGCCACCACG CCCAGCTAATTTTTATATTTTTAGTACAGATGGGGTTTCACCATGTTGGCCAGGCTGGTC TCGAACTCCTGACCTCAAGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATAATAG GTGTCAGCCACCGCACCCAGCCTGGAGTGGCCTTTTATGAGAGGGGACCCGTCAAATCTG TGCCTTATGGAGGGGTCCGGCAGCGGCCACAATTGTCTTGTCCCCTCACCCCCCAACTCC CCCTGGAACACCTCTCCCAGGCAAGACATTTTCACAGCACCATTCACAACGGTTGGGCCA AAAAGAAACTTTTCCTTCATCATTTCCTGCACTCGCTGACCACAACTTTGGACACCCCAG GCTGCCACCCCTCCCCACCCGTTCACCCCCAGGATGCTGTTGCTGTAGGACGCCTGCTGC CCTGGAGCCCTCCCCAGGATGTGAGCCAGTCCCCTCGCTGGTACGGAATGCCGCTGGGTG CCCGGAGGCGGCCATGGTGTCTCGATGGACGGCAGCCAGGATGGAGCACCCATGGGTCTC ACGGCCATGCTTCAGGGTCTTCAGGTCCTGCCCCCGGCCAGTCTGCCAAGAGGCACCCCC TTCCCCAGCCTCTCGCCTGCACTGATGCAGACAAAATCTCACCTGGCAGGCCCAACCCCC ACCCCACCCCTCCCCCGCCGTGTGTGGCCCCTCGCCGCATCGTTGGGGTTTTGTTACGTG AAAATATCCTGGAAATAAATACATGTTTCTGCACTTAGAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC062598 unedited AGTCAGAATTGTATACGACTCACTATAGGCGGCCGCGATTCANATCTGGTACCGGTCCGG AATTCCCGGGATGACTTGGCGAAGCGCTGCGCTCGCGCCCGGATCCCTCAGGCGGCTGCA GGCTTCAGCCTGCGCTGGTTGGTGAAACAGAGATGTCAGAAAAGGAGAACAACTTCCCGC CACTGCCCAAGTTCATCCCTGTGAAGCCCTGCTTCTACCAGAACTTCTCCGACGAGATCC CAGTGGAGCACCAGGTCCTGGTGAAGAGGATCTACCGGCTGTGGATGTTTTACTGCGCCA CCCTCGGCGTCAACCTCATTGCCTGCCTGGCCTGGTGGATCGGCGGAGGCTCGGGGACCA ACTTCGGCCTGGCCTTCGTGTGGCTGCTCCTGTTCACGCCTTGCGGCTACGTGTGCTGGT TCCGGCCTGTCTACAAGGCCTTCCGAGCCGACAGCTCCTTTAATTTCATGGCGTTTTTCT TCATCTTCGGAGCCCAGTTTGTCCTGACCGTCATCCAGGCGATTGGCTTCTCCGGCTGGG GCGCGTGCGGCTGGCTGTCGGCAATTGGATTCTTCCAGTACAGCCCGGGCGCTGCCGTGG TCATGCTGCTTCCAGCCATCATGTTCTCCGTGTCGGCTGCCATGATGGCCATCGCGATCA TGAAGGTGCACAGGATCTACCGAGGGGCTGGCGGAAGCTTCCAGAAGGCACAGACGGAGT GGAACACGGGCACTTGGGCGAACCCACCGTCGAGGGAGGCCCAGTACAACAACTTCTCAG GCAACAGCCTGCCCGAGTACCCCACTGTGCCCAGCTACCCGGGCAGTGGCCAGTGGCCTT AGAGGGAGCCTGCCCTGCCCCACCGCCCACACCTCCTCCCTTCATACTGCTGCTACCCTG A
Restriction Sites	NotI-NotI
ACCN	BC062598
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062598.1, AAH62598.1
RefSeq Size	2585 bp
Locus ID	113178
Gene Summary	Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Most SCAMPs (e.g., SCAMP1; MIM 606911) have N-terminal cytoplasmic NPF (arg-pro-phe) repeats, 4 central transmembrane regions, and a short C-terminal cytoplasmic tail. These SCAMPs likely have a role in endocytosis that is mediated by their NPF repeats. Other SCAMPs, such as SCAMP4, lack the NPF repeats and are therefore unlikely to function in endocytosis (summary by Fernandez-Chacon and Sudhof, 2000 [PubMed 11050114]).[supplied by OMIM, Feb 2011]

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