p40 (RABEPK) (BC000503) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA65N13.1; DKFZp686P1077; OTTHUMP00000022126; p40; Rab9 effector p40; Rab9 effector protein with kelch motifs; RAB9P40
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000503 edited GGCGAGGGTCCCCGGATACCGGGTCTATCACAGTCTCGGGCAGGGAGTCTGAATCTTTTA GGGGAGTGGGCCCAAGCCGGGTGCAAAAAACGGGGAAGGGCCTTCCCTGGCTCCGTCCCG GCCACTTTGACCGAATCAGCCTGTTCTTTCCCGACCCCGTCTCCTATCCCCTAGAACTGC CACGTGGGGATGAGATTTGCTGGGCTGGTAGCGGCGGCTGCTGCGGGAGGTCCCGCCCAC GTGAAGCCAGCCTAACTGAGCTCTGGACTTTGGGGACAGCTGTCAGTGGCCTAGGCCGCA GGACACCATGAAGCAACTGCCAGTCTTGGAACCTGGAGACAAGCCCAGGAAAGCAACATG GTACACCTTGACTGTCCCTGGAGACAGCCCCTGTGCTCGAGTTGGCCACAGCTGTTCATA TTTACCCCCAGTTGGTAATGCCAAGAGAGGGAAGGTCTTCATTGTTGGGGGAGCAAATCC AAACAGAAGCTTCTCAGACGTGCACACCATGGATCTGGGAAAATACCAGTGGGACTTAGA TACCTGCAAGGGCCTCTTGCCCCGGTATGAACATGCTAGCTTCATTCCCTCCTGCACACC TGACCGTATCTGGGTATTTGGAGGTGCCAACCAATCAGGAAATCGAAATTGTCTACAAGT CCTGAATCCTGAAACCAGGACGTGGACCACGCCAGAAGTGACCAGCCCCCCACCATCCCC AAGAACATTCCACACATCATCGGCAGCCATTGGAAACCAGCTATATGTCTTTGGGGGCGG AGAGAGAGGTGCCCAGCCCGTGCAGGACACGAAGCTGCATGTGTTTGACGCAAACACTCT GACCTGGTCACAGCCAGAGACACTTGGAAATCCTCCATCTCCCCGGCATGGTCATGTGAT GGTGGCAGCAGGGACAAAGCTCTTCATCCACGGAGGCTTGGCGGGGGACAGATTCTATGA TGACCTCCACTGCATTGATATAAGTGACATGAAATGGCAGAAGCTAAATCCCACTGGGGC TGCTCCAGCAGGCTGTGCTGCCCACTCAGCTGTGGCCATGGGAAAACATGTGTACATCTT TGGTGGAATGACTCCTGCAGGAGCACTGGACACAATGTACCAGTATCACACAGAAGAGCA GCATTGGACCTTGCTTAAATTTGATACTCTTCTACCCCCTGGACGATTGGACCATTCCAT GTGTATCATTCCATGGCCAGTGACGTGTGCTTCTGAGAAAGAAGGTTCCAACTCTCTCAC TCTGAACCATGAAGCTGAGAAAGAGGATTCAGCTGACAAAGTAATGAGCCACAGTGGTGA CTCACATGAGGAAAGCCAGACTGCTACACTGCTCTGTTTGGTGTTTGGTGGGATGAATAC AGAAGGGGAAATCTATGACGATTGTATTGTGACTGTAGTGGACTAATAAAACCCACATTT TTATTACCAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000503 unedited TTTGCGTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCGAG GGTCCCCGGNAACCGGGTCTATCACAGTCTCGGGCAGGGAGTCTGAATCTTTTAGGGGAG TGGGCCCAAGCCGGGTGCAAAAAACGGGGAAGGGCCTTCCCTGGCTCCGTCCCGGCCACT TTGACCGAATCAGCCTGTTCTTTCCCGACCCCGTCTCCTATCCCCTAGAACTGCCACGTG GGGATGAGATTTGCTGGGCTGGTAGCGGCGGCTGCTGCGGGAGGTCCCGCCCACGTGAAG CCAGCCTAACTGAGCTCTGGACTTTGGGGACAGCTGTCAGTGGCCTAGGCCGCAGGACAC CATGAAGCAACTGCCAGTCTTGGAACCTGGAGACAAGCCCAGGAAAGCAACATGGTACAC CTTGACTGTCCCTGGAGACAGCCCCTGTGCTCGAGTTGGCCACAGCTGTTCATATTTACC CCCAGTTGGTAATGCCAAGAGAGGGAAGGTCTTCATTGTTGGGGGAGCAAATCCAAACAG AAGCTTCTCAGACGTGCACACCATGGATCTGGGAAAATACCAGTGGGACTTAGATACCTG CAAGGGCCTCTTGCCCCGGTATGAACATGCTAGCTTCATTCCCTCCTGCACACCTGACCG TATCTGGGTATTTGGGAGGTGCCAACCCAATCAGGAAATCGAAAATGTCTACAAGTCCCT GATCCTGAAACCAGGACGTGGACCACGCCANAAATGACCAANCCCCCCACATACCCCAAA ACATCCCCACATCATCGGCCGCCAATGGGGAACCGCTATTAGTCTTTGGGGGCCGAAAAA GAATGGCCCCACCCCTGCCG
Restriction Sites	NotI-NotI
ACCN	BC000503
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000503.2, AAH00503.1
RefSeq Size	1466 bp
Locus ID	10244
Protein Families	Druggable Genome
Gene Summary	Rab9 effector required for endosome to trans-Golgi network (TGN) transport.[UniProtKB/Swiss-Prot Function]

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