BCDIN3D (NM_181708) Human Untagged Clone
CAT#: SC126373
BCDIN3D (untagged)-Human BCDIN3 domain containing (BCDIN3D)
CNY 6,270.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_181708 edited
GGCTAATGGCGGTGCCCACGGAACTGGATGGAGGGAGTGTTAAGGAGACCGCAGCGGAAG AGGAATCGCGAGTTCTGGCACCTGGCGCCGCCCCGTTCGGAAATTTTCCTCATTATTCTC GCTTCCACCCTCCGGAGCAACGGCTCCGCCTCCTGCCCCCGGAGCTGCTTCGACAGCTCT TTCCTGAGAGTCCCGAGAACGGGCCGATTCTGGGGCTCGACGTGGGGTGTAACTCCGGGG ATCTGAGTGTGGCTCTATACAAACACTTCCTCTCCCTACCTGACGGGGAAACCTGCTCAG ATGCCTCAAGAGAATTCCGTCTCCTCTGCTGCGACATAGATCCAGTCCTGGTGAAGCGAG CCGAAAAAGAATGTCCTTTTCCTGATGCCTTGACTTTTATCACCCTGGACTTCATGAATC AAAGGACCCGGAAGGTTCTCTTGAGCTCTTTCTTAAGCCAATTTGGACGTTCAGTTTTTG ACATTGGCTTCTGCATGTCAATAACCATGTGGATTCATCTGAATCATGGAGACCATGGCC TATGGGAGTTCCTGGCCCATCTTTCCTCCCTCTGCCACTACCTCCTTGTGGAGCCCCAAC CCTGGAAGTGTTACCGGGCAGCTGCAAGGCGTCTCCGAAAGCTGGGACTCCATGATTTTG ACCACTTCCACTCCCTTGCCATCCGAGGTGACATGCCCAATCAGATTGTGCAGATCTTGA CCCAGGATCATGGCATGGAATTAATATGTTGCTTTGGCAACACCAGTTGGGACAGAAGCC TTCTGCTCTTCAGGGCAAAACAAACCATAGAGACTCATCCAATCCCTGAATCACTGATAG AAAAAGGGAAAGAAAAGAACAGATTAAGTTTCCAGAAGCAGTGAGATGGGAGGGCAAGAA GACCAAGAAAGATATTGAAAGGGTTTTATATTGAGAATTATGTTCATTCTCCTTAATTCC TTAACAATCAGGCAGCCGCAAAACCTGGCAGGAGCTTTTGGTAAAATGTCCAAGGCACAT AATTGAAAGAATCAGTATCTGTTCCCCATTGTTTGGAACAAATCTTGAGGAGAATGAATC TGTGGAGTGATGAGCTAGGATATCTGGATTGAGATGGCCTAGGAAAAGTGGTCCTGGTTA TGGGTTATGGAGAGGGCTGCTTTTGGGGATTATTTATAAAGATCAGACTAGGCTAGTCTT AAAATATGTCAGTTCCTGGGCTCTCTGAATCCTACCTGGTTTCTTCAGGCTTCTGGACTT GCTAGGCCTGCCTTTGGGCAAAACCTTAGTAATTCAGGTCTGGAATGGGGCTTAGGAATC TATTTTCTGTTTTTTTGTTTGTTTGTTTTTGCATTCTTCTATTGTAATATATACATAAAA TTTACCATTTTAACCGTTTTTAAGTACAGTTTTTCAGAACGTGGAAATCCCATCAAAAAT TGGATTAAAAAATTAAAAGATAAATTGATAATTTTTTTAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_181708 unedited
GTTTTGATTTGTATACGACTTACTATAGGCGGCCGCGATTCAGATCTGGTACCGGTCCGG AATTCCCGGGATATCGTCGACCCACGCGTCCGGGCTAATGGCGGTGCCCACGGAACTGGA TGGAGGGAGTGTTAAGGAGACCGCAGCGGAAGAGGAATCGCGAGTTCTGGCACCTGGCGC CGCCCCGTTCGGAAATTTTCCTCATTATTCTCGCTTCCACCCTCCGGAGCAACGGCTCCG CCTCCTGCCCCCGGAGCTGCTTCGACAGCTCTTTCCTGAGAGTCCCGAGAACGGGCCGAT TCTGGGGCTCGACGTGGGGTGTAACTCCGGGGATCTGAGTGTGGCTCTATACAAACACTT CCTCTCCCTACCTGACGGGGAAACCTGCTCAGATGCCTCAAGAGAATTCCGTCTCCTCTG CTGCGACATAGATCCAGTCCTGGTGAAGCGAGCCGAAAAAGAATGTCCTTTTCCTGATGC CTTGACTTTTATCACCCTGGACTTCATGAATCAAAGGACCCGGAAGGTTCTCTTGAGCTC TTTCTTAAGCCAATTTGGACGTTCAGTTTTTGACATTGGCTTCTGCATGTCAATAACCAT GTGGATTCATCTGAATCATGGAGACCATGGCCTATGGGAGTTCCTGGCCCATCTTTCCTC CCTCTGCCACTACCTNCTTGTGGAGCCCCAACCCTGGNAAGTGTACCGGGCAGCTGCAAG GCGTCTCCGANAGCTGGGACTCCATGATTTTGACCACTTNCACTNCCTTGCCATNNCGAG TGACATGCCCAATCAGATTGTGCAGATCTTGACCCCAGATCATGGCATGGAATTNATATG NNTGCTTNNGCACACCAGTGGGACAGAAGCCTTCTGCTCTNCAGGGCAAACAAACATAGA GACTCTTCCATCCCCTGATACC |
Restriction Sites | NotI-NotI |
ACCN | NM_181708 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_181708.1, NP_859059.1 |
RefSeq Size | 1495 bp |
RefSeq ORF | 879 bp |
Locus ID | 144233 |
UniProt ID | Q7Z5W3 |
Gene Summary | This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. [provided by RefSeq, Apr 2017] |
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