Nesprin3 (SYNE3) (BC061899) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	chromosome 14 open reading frame 49; FLJ25605; MGC75267; nesprin-3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC061899 edited CTAGAAGACATCAAAGTGCTGTTTTACAGCAAGGGTTGGCAGACTGTTTCTGTGAAGGGC CAGACAGTGAAGGTTTTAGAGTCTGCAGGGCAGGCAGTCGCTGTCGCAGCTCCTCAGCGC CACCACTGCAGCTATAAATGTATGGGCATGGCTGGGTTCCTATAAAACTTTATTTACAAA AACAGGCCTCTGGTGGGATTTGGTCGGTGGGTGGAGGGGTGTAGCTTGCCCCAGTTCTGG AGGGATGTGGCCAAAGTGTTCTGTGAGGACGCTGTAGTGACCCTAAAGAGAGGCACCCAA CTCCATCAGGGCTCAGGGAAGGCTTCCTGGAGGGGGTGGCGCCTGAATGAGCATGAGCTG GCCGAGACGAAGAGCAGCCTGGCTGAACGGGTCGGGTCTCACAACACGGTGCTCATGAGG AACGCTCTTAATGTGCACACTGTCCTGTGTCTGCAGAGCCTGCTCGCTCAGCACAAAGAC TTTGGAGCAGCTTTTGAGCCCCTGCAGAGGAAGCTCTTGGACCTCCAGGTCAGGGTCCAA GCCGAGAAGGGGCTTCAGCGGGACCTTCCTGGAAAACAGGCCCAGCTCTCAAGGTTGCAG GTGAGGGGTCTGTGAGGCCTGAATCACTCCTGCCCAGGTGCTCAGCTGCACCTGGAAGCA GCGTCTCCCTCTAGCAGTAACCAGCAAAGCAACAGCTGCCGTGAGCCAGAAATTTAGACC CTTAGTTGAGATCTGACCTTTCACCTCAACAGAACCTCTGCTTATTTATCTGATGGTTCC TGACCTCAAACAACTGAGTCTTGAGCTCAGCAAAATGACAAACTGTCTTTCTGTTTACTC CTCAGTTCCAAAGTCTAACATTGGTATTTGTTTTTAGAATTGCTTTGCTAAGTATTCTTA ATGAGTCCAGAGGTGTTCAGTTAAACCAACTTTGGGATTAAGAATAAATGTTCTGAAACG TGGACTGTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC061899 unedited NGGGCGGTTCAGATTTTGTAATACGACTTACTATAGGCGGACCGCGATTCAGATCTGGTA CCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCTAGAAGACATCAAAGTGC TGTTTTACAGCAAGGGTTGGCAGACTGTTTCTGTGAAGGGCCAGACAGTGAAGGTTTTAG AGTCTGCAGGGCAGGCAGTCGCTGTCGCAGCTCCTCAGCGCCACCACTGCAGCTATAAAT GTATGGGCATGGCTGGGTTCCTATAAAACTTTATTTACAAAAACAGGCCTCTGGTGGGAT TTGGTCGGTGGGTGGAGGGGTGTAGCTTGCCCCAGTTCTGGAGGGATGTGGCCAAAGTGT TCTGTGAGGACGCTGTAGTGACCCTAAAGAGAGGCACCCAACTCCATCAGGGCTCAGGGA AGGCTTCCTGGAGGGGGTGGCGCCTGAATGAGCATGAGCTGGCCGAGACGAAGAGCAGCC TGGCTGAACGGGTCGGGTCTCACAACACGGTGCTCATGAGGAACGCTCTTAATGTGCACA CTGTCCTGTGTCTGCAGAGCCTGCTCGCTCAGCACAAAGACTTTGGAGCAGCTTTTGAGC CCCTGCAGAGGAAGCTCTTGGACCTCCAGGTCAGGGTCCAAGCCGAGAAGGGGCTTCAGC GGGACCTTCCTGGANAACAGGCCCAGCTCTCAAGGTTGCAGGTGAGGGGTCTGTGAGGCC TGAATCACTCCTGCCCAGGTGCTCAGCTGCACCTGGAAGCAGCGTCTCCCTCTAGCAGTA ACCAGCAAAGCAACAGCTGCCGTGAGCCAGAAATTTAGACCCTTAGTTGAGATCTGACCT TTTCACCTCACAGAACCTCTGCTTATTTATCTGATGGGTCCTGACCTCAAACAACTG
Restriction Sites	Please inquire
ACCN	BC061899
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC061899.1, AAH61899.1
RefSeq Size	983 bp
RefSeq ORF	201 bp
Locus ID	161176
Protein Families	Transmembrane
Gene Summary	As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.[UniProtKB/Swiss-Prot Function]

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