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B Raf (BRAF) (NM_004333) Human Untagged Clone
CAT＃: SC126575
BRAF (untagged)-Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
CNY 8,424.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B-raf; B-RAF1; BRAF1; NS7; RAFB1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126575 sequence for NM_004333 edited (data generated by NextGen Sequencing)
ATGGCGGCGCTGAGCGGTGGCGGTGGTGGCGGCGCGGAGCCGGGCCAGGCTCTGTTCAAC GGGGACATGGAGCCCGAGGCCGGCGCCGGCGCCGGCGCCGCGGCCTCTTCGGCTGCGGAC CCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAAGTTGACACAGGAACAT ATAGAGGCCCTATTGGACAAATTTGGTGGGGAGCATAATCCACCATCAATATATCTGGAG GCCTATGAAGAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTG GAATCTCTGGGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTT ACATCTTCTTCCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAAT CCCACAGATGTGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTC CTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT CTAAAGAAAGCACTGATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATT CAGGATGGAGAGAAGAAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAA GAATTGCATGTGGAAGTGTTGGAGAATGTTCCACTTACAACACACAACTTTGTACGAAAA ACGTTTTTCACCTTAGCATTTTGTGACTTTTGTCGAAAGCTGCTTTTCCAGGGTTTCCGC TGTCAAACATGTGGTTATAAATTTCACCAGCGTTGTAGTACAGAAGTTCCACTGATGTGT GTTAATTATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAACACCACCCAATA CCACAGGAAGAGGCGTCCTTAGCAGAGACTGCCCTAACATCTGGATCATCCCCTTCCGCA CCCGCCTCGGACTCTATTGGGCCCCAAATTCTCACCAGTCCGTCTCCTTCAAAATCCATT CCAATTCCACAGCCCTTCCGACCAGCAGATGAAGATCATCGAAATCAATTTGGGCAACGA GACCGATCCTCATCAGCTCCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGAT GACTTGATTAGAGACCAAGGATTTCGTGGTGATGGAGGATCAACCACAGGTTTGTCTGCT ACCCCCCCTGCCTCATTACCTGGCTCACTAACTAACGTGAAAGCCTTACAGAAATCTCCA GGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAATCGAATGAAAACA CTTGGTAGACGGGACTCGAGTGATGATTGGGAGATTCCTGATGGGCAGATTACAGTGGGA CAAAGAATTGGATCTGGATCATTTGGAACAGTCTACAAGGGAAAGTGGCATGGTGATGTG GCAGTGAAAATGTTGAATGTGACAGCACCTACACCTCAGCAGTTACAAGCCTTCAAAAAT GAAGTAGGAGTACTCAGGAAAACACGACATGTGAATATCCTACTCTTCATGGGCTATTCC ACAAAGCCACAACTGGCTATTGTTACCCAGTGGTGTGAGGGCTCCAGCTTGTATCACCAT CTCCATATCATTGAGACCAAATTTGAGATGATCAAACTTATAGATATTGCACGACAGACT GCACAGGGCATGGATTACTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAAT AATATATTTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACAGTG AAATCTCGATGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCCATTTTGTGGATG GCACCAGAAGTCATCAGAATGCAAGATAAAAATCCATACAGCTTTCAGTCAGATGTATAT GCATTTGGAATTGTTCTGTATGAATTGATGACTGGACAGTTACCTTATTCAAACATCAAC AACAGGGACCAGATAATTTTTATGGTGGGACGAGGATACCTGTCTCCAGATCTCAGTAAG GTACGGAGTAACTGTCCAAAAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAGAAA AGAGATGAGAGACCACTCTTTCCCCAAATTCTCGCCTCTATTGAGCTGCTGGCCCGCTCA TTGCCAAAAATTCACCGCAGTGCATCAGAACCCTCCTTGAATCGGGCTGGTTTCCAAACA GAGGATTTTAGTCTATATGCTTGTGCTTCTCCAAAAACACCCATCCAGGCAGGGGGATAT GGTGCGTTTCCTGTCCACTGA Clone variation with respect to NM_004333.4 >OriGene 5' read for NM_004333 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCCGGTACCTGAG GTGGCCCAGGCGCCCTCCGCCCGCGGCGCCGCCCGGGCCGCTCCTCCCCGCGCCCCCCGC GCCCCCCGCTCCTCCGCCTCCGCCTCCGCCTCCGCCTCCCCCAGCTCTCCGCCTCCCTTC CCCCTCCCCGCCCGACAGCGGCCGCTCGGGCCCCGGCTCTCGGTTATAAGATGGCGGCGC TGAGCGGTGGCGGTGGTGGCGGCGCGGAGCCGGGCCAGGCTCTGTTCAACGGGGACATGG AGCCCGAGGCCGGCGCCGGCGCCGGCGCCGCGGCCTCTTCGGCTGCGGACCCTGCCATTC CGGAGGAGGTGTGGAATATCAAACAAATGATTAAGTTGACACAGGAACATATAGAGGCCC TATTGGACAAATTTGGTGGGGAGCATAATCCACCATCAATATATCTGGAGGCCTATGAAG AATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGG GGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTT CCTCTTCTAGCCTTTCAGTGCTACCTTCATCNTCTTCAGTTTTTCAAAATCCCACAGATG TGGCACGGAGCACCCCCCAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCACAA ACAGAGGACAGTGGTACCTGCCAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGC CTGATGATGAGAAGTCTAATCCAGAGTGCTGTGCTGTTACAGAATCCAGATGAGGAGAGA ACCAATTGTTGGGACACTGAATTCCTGGCTACTGGAAAAAATGCCGGGGAAGGGTGGAGA TGCCCCTTACCC >OriGene 3' read for NM_004333 unedited
GTAACGCGGCCCGCAATCTAGTAGTCGAGTTTTTTTTTTTTTTTTTTTAACATATAAGCA AACATATGTTCATTTATTTTCCTTTCGTTGCTACTCTCCTGAACTCTCTCACTCATTTGT TTCAGTGGACAGGAAACGCACCATATCCCCCTGCCTGGATGGGTGTTTTTGGAGAAGCAC AAGCATATAGACTAAAATCCTCTGTTTGGAAACCAGCCCGATTCAAGGAGGGTTCTGATG CACTGCGGTGAATTTTTGGCAATGAGCGGGCCAGCAGCTCAATAGAGGCGAGAATTTGGG GAAAGAGTGGTCTCTCATCTCTTTTCTTTTTGAGGCACTCTGCCATTAATCTCTTCATGG CTTTTGGACAGTTACTCCGTACCTTACTGAGATCTGGAGACAGGTATCCTCGTCCCACCA TAAAAATTATCTGGTCCCTGTTGTTGATGTTTGAATAAGGTAACTGTCCAGTCATCAATT CATACAGAACAATTCCAAATGCATATACATGCTGACTGAAAGCTGTATGGATTTTTATCT TGCATTCTGATGACTTCTGGTGCCATTCACAAAATGGATCCAGACAACTGTTCAAACTGA TGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGGG AGGTCTTCATGAAGAATATATTATTACTCTTGAGGTCTCTTGAGGATGATTGACTTGGCG TGTAGTAATCCATGCCCCTGGCAGTCTGCCCCGCCATAATCATAAAGTTGATCTCTCAAA TTTGGCCCAAAGATTTGGAGATGGTGATACAAGCCGGAGCCCTCCACCACTGGGTACAAT AGCCAGTTGTGGCTTTGTGGATAACCCCATGAAATAGGATTTTCACTGGGGGGGTTTACC TAAACCCCTATTCCTTTTTGAAGCTCGAACTGCCAAGGGTAGGGCCGTCAATTAACTTTT ACCGGCCATACCATTCATTTCCCTTGTAACAGTACACAAACCAAACCATCTTGGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004333 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004333.2, NP_004324.2 |
| RefSeq Size | 2513 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 673 |
| UniProt ID | P15056 |
| Domains | pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction |
| Gene Summary | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211013 | BRAF (Myc-DDK-tagged)-Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) |
CNY 8,416.00 |
|
| RC211013L1 | Lenti ORF clone of Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), Myc-DDK-tagged |
CNY 10,816.00 |
|
| RC211013L2 | Lenti ORF clone of Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), mGFP tagged |
CNY 7,700.00 |
|
| RC211013L3 | Lenti ORF clone of Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), Myc-DDK-tagged |
CNY 10,816.00 |
|
| RC211013L4 | Lenti ORF clone of Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), mGFP tagged |
CNY 10,816.00 |
|
| RG211013 | BRAF (tGFP-tagged) - Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) |
CNY 10,016.00 |
