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gamma Adducin (ADD3) (NM_016824) Human Untagged Clone
CAT＃: SC126647
ADD3 (untagged)-Human adducin 3 (gamma) (ADD3), transcript variant 1
CNY 5,176.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADDL; CPSQ3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_016824, the custom clone sequence may differ by one or more nucleotides
ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGAT ATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACA AGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGAC TTGGAATGCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGA TTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCAC ACCTATCAATGACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTATATCTCAGTAA GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAAGCTACAGCCTC CAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGACCAGGGAAGTACCAATTTGAAAATTGACCAT ACAGGATTCAGTCCCCATGCTGCAATCTATTCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATA CCCTTGCAACAGCAGCTGTATCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCT GGGAGATGTTGCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAG AGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTGCAGGCCCTAGCAGGTGC AGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAGTATAAAGCTTTCACTTACACTGTAGCAGCG TCTGGTGGAGGAGGTGTGAATATGGGTTCCCATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGC TTATGAGGACTCTGGACAACTTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAA GCCTAGGCACAAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTGAACTCACCAA ATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACCAGTCCCCGAACCAAAATCAC GTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGAACACCTATCAAAATTGAAGATCCAAATCAG TTTGTTCCTTTAAACACAAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGAT ATGACTTGAAAACAGCAGGACCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTAC TATGCAATTTGAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGATGCTGAGCAGGAATTAC TCTCAGATGACGCTTCATCTGTTTCACAAATTCAGTCTCAAACTCAGTCACCGCAAAATGTCCCTGAAAA ATTAGAAGAAAACCATGAGCTGTTTTCCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAAT GGCAAGGATGATATGCATGATGTTGAAGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAA CCATAGAAAACATCGAGATTACTATTAAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTC CCCTTCAAAATCGCCATCCAAGAAAAAGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAA AAGGAGAAAGTTGAGGCCTAA >OriGene 5' read for NM_016824 unedited
TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCGTAACG GTCGCCCAGTGTGAGGGGGCGGGNNAGGGAAAGAAGAGGGGTTTAAATTAGATTTTTTAA AACACAGAGCAAGCGCCAGAGGCGTCGGCATCCCAGGTGTCGCCGCTTCCTGCTGCACAG GGCTCGGCGTACAGGTCCCTCCCTCCTCAAGCCCCCTCCCCTTCTCCCGCCCTACCCTCT GGGGCTCTGCGGCGCTTAAGAGGCGGCCGCAGCGGCGGATCCGGCGGCTGCTGCAGCCCG GGCGGCTGCCGAGAAGGAGGGAGGGGAAACACAAAGCCGGCTACGCGCTGCGAGATAACA AGAGTAATCCACAGACTTAAAACATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTC CTCCTCCTCCCAGCATGCCTCACAAAGAGAGATATTTTGACCGCATCAATGAAAATGACC CAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACAAGACTTCAACATGATGG AGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGACTTGGAAT GCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGC AGATTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTA AGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAA GCTACAGCCTNCAATTTGGNTGAAAGTCAATATATAGGGAAAAGTGGTTGACCCGGGAAG TACCCAATTGAAAAATGACCATACAGGATTCAGTCCCCATGCTGCAATCTATTCACACGT NCTGATGTTAAAGTGTGTGATACACATCCATC >OriGene 3' read for NM_016824 unedited
NNTTTTAGCTTGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCCAG AGTTAAACTAAATAACCCATGTTATGTGTACAAAATCCCCTTTGTTGAAAAATAAGGGGC TTTCTAAACTAATAAAAAAGGAAGTTTTCAAAAATTATAGTTTATTAAAACAACTTTTTT GGCAAACAAAGTTACTTCAGGTGAGGAAATTTTATACTGTGAATAAAATTCCAAATGAAT CTTTTCTTAAAACTTTTTAAAAAATTATGTGCCAGTGTATACTAATGCTATAGATTCTTG TCTTAGAAGTTTTTAAAGCATTCTGTTAATGCCCACTGAAACAATGGGACTCCAAAAATA TAGTCAATAATCATGATAAAAAATTATAATATGATTATCAAGTGAAGCAGGTATTGAGAA ATAAAAATTCTCACTTGCTCACTGGCAATTTCTTTCTAACAGATATTATGGAGAAGGCCT GAAGTAATTCAGACAGATAGCTGTTTATGGTGAATTATAATAATACTTCATGAGGGCAGA GCTAATTAAAACTAGTAATTGCTTAAAATTCAAAGCCATTTCTGGACATATAAAATGAGA GATGAATCTGAAAGTTTTTCTTTTGTAAAACCTCTTTCCAGTTCTTAAAGTCCAGTTTGC TACATCCCCCAATCTGATCTACCATTGCATATTAATGATCAACTTAAATGTGGTATTTAG ATGTGCAATGTCACATTGTTATAATAATAATTATAAAAAGACTCTATTTAGGCCTCAACT TTCTCCTTTTTTTTGGTCCTTTTTCAGAAAAGAAGGAGTGCGGAATTTCTTCTTTTTCTT GGATGCCGATTTTGAAGGGNAGCCTTCACGTGACAGGACTCCTTCGATTNTCTCTGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016824 |
| Insert Size | 2770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016824.3, NP_058432.1 |
| RefSeq Size | 4454 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 120 |
| UniProt ID | Q9UEY8 |
| Domains | Aldolase_II |
| Gene Summary | Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 4-6 encode the same isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209129 | ADD3 (Myc-DDK-tagged)-Human adducin 3 (gamma) (ADD3), transcript variant 1 |
CNY 5,168.00 |
|
| RC209129L1 | Lenti ORF clone of Human adducin 3 (gamma) (ADD3), transcript variant 1, Myc-DDK-tagged |
CNY 7,568.00 |
|
| RC209129L2 | Lenti ORF clone of Human adducin 3 (gamma) (ADD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209129L3 | Lenti ORF clone of Human adducin 3 (gamma) (ADD3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209129L4 | Lenti ORF clone of Human adducin 3 (gamma) (ADD3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209129 | ADD3 (tGFP-tagged) - Human adducin 3 (gamma) (ADD3), transcript variant 1 |
CNY 6,768.00 |
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