TXNDC13 (TMX4) (BC021964) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DJ971N18.2; PDIA14; TXNDC13
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC021964, the custom clone sequence may differ by one or more nucleotides GAGCGAGGCTGCGGGCCGGCCGCGTGCCCTTCCCCACACTCCCCGCCGAGAAGCCTCGCTCGGCGCCCAA CATGGCGGGTGGGCGCTGCGGCCCGCAGCTAACGGCGCTCCTGGCCGCCTGGATCGCGGCTGTGGCGGCG ACGGCAGGCCCCGAGGAGGCCGCGCTGCCGCCGGAGCAGAGCCGGGTCCAGCCCATGACCGCCTCCAACT GGACGCTGGTGATGGAGGGCGAGTGGATGCTGAAATTTTACGCCCCATGGTGTCCATCCTGCCAGCAGAC TGATTCAGAATGGGAGGCTTTTGCAAAGAATGGTGAAATACTTCAGATCAGTGTGGGGAAGGTAGATGTC ATTCAAGAAGCCAGGTTTGAGTGGCCGCTTCTTTGTCACCACTCTCCCAGCATTTTTTCATGCAAAGGAT GGGATATTCCGCCGTTATCGTGGCCCAGGAATCTTCGAAGACCTGCAGAATTATATCTTAGAGAAGAAAT GGCAATCAGTCGAGCCTCTGACTGGCTGGAAATCCCCGGCTTCTCTAACGATGTCTGGAATGGCTGGTCT TTTTAGCATCTCTGGCAAGATATGGCATCTTCACAACTATTTCACAGTGACTCTTGGAATTCCTGCTTGG TGTTCTTATGTCTTTTTCGTCATAGCCACCTTGGTTTTTGGCCTTTTTATGGGTCTGGTCTTGGTGGTAA TATCAGAATGTTTCTATGTGCCACTTCCAAGGCATTTATCTGAGCGTTCTGAGCAGAATCGGAGATCAGA GGAGGCTCATAGAGCTGAACAGTTGCAGGATGCGGAGGAGGAAAAAGATGATTCAAATGAAGAAGAAAAC AAAGACAGCCTTGTAGATGATGAAGAAGAGAAAGAAGATCTTGGCGATGAGGATGAAGCAGAGGAAGAAG AGGAGGAGGACAACTTGGCTGCTGGTGTGGATGAGGAGAGAAGTGAGGCCAATGATCAGGGGCCCCCAGG AGAGGACGGTGTGACCCGGGAGGAAGTAGAGCCTGAGGAGGCTGAAGAAGGCATCTCTGAGCAACCCTGC CCAGCTGACACAGAGGTGGTGGAAGACTCCTTGAGGCAGCGTAAAAGTCAGCATGCTGACAAGGGACTGT AGATTTAATGATGCGTTTTCAAGAATACACACCAAAACAATATGTCAGCTTCCCTTTGGCCTGCAGTTTG TACCAAATCCTTAATTTTTCCTGAATGAGCAAGCTTCTCTTAAAAGATGCTCTCTAGTCATTTGGTCTCA TGGCAGTAAGCCTCATGTATACTAAGGAGAGTCTTCCAGGTGTGACAATCAGGATATAGAAAAACAAACG TAGTGTTGGGATCTGTTTGGAGACTGGGATGGGAACAAGTTCATTTACTTAGGGGTCAGAGAGTCTCGAC CAGAGGAGGCCATTCCCAGTCCTAATCAGCACCTTCCAGAGACAAGGCTGCAGGCCCTGTGAAATGAAAG CCAAGCAGGAGCCTTGGCTCCTGAGCATCCCCAAAGTGTAACGTAGAAGCCTTGCATCCTTTTCTTGTGT AAAGTATTTATTTTTGTCAAATTGCAGGAAACATCAGGCACCACAGTGCATGAAAAATCTTTCACAGCTA GAAATTGAAAGGGCCTTGGGTATAGAGAGCAGCTCAGAAGTCATCCCAGCCCTCTGAATCTCCTGTGCTA TGTTTTATTTCTTACCTTTAATTTTTCCAGCATTTCCACCATGGGCATTCAGGCTCTCCACACTCTTCAC TATTATCTCTTGGTCAGAGGACTCCAATAACAGCCAGGTTTACATGAACTGTGTTTGTTCATTCTGACCT AAGGGGTTTAGATAATCAGTAACCATAACCCCTGAAGCTGTGACTGCCAAACATCTCAAATGAAATGTTG TGGCCATCAGAGACTCAAAAGGAAGTAAGGATTTTACAAGACAGATTAAAAAAAAATGGTTTTGTCCAAA ATATAGTTGTTGTTGATTTTTTTTTAAGTTTTCTAAGCAATATTTTTCAAGCCAGAAGTCCTCTAAGTCT TGCCAGTACAAGGTAGTCTTGTGAAGAAAAGTTGAATACTGTTTTGTTTTCATCTCAAGGGGTTCCCTGG GTCTTGAACTACTTTAATAATAACTAAAAAACCACTTCTGATTTTCCTTCAGTGATGTGCTTTTGGTGAA AGAATTAATGAACTCCAGTACCTGAAAGTGAAAGATTTGATTTTGTTTCCATCTTCTGTAATCTTCCAAA GAATTATATCTTTGTAAATCTCTCAATACTCAATCTACTGTAAGTACCCAGGGAGGCTAATTTCCTTAAA AAAAAAAAATCTATCCATCTACTTCTCTCTTACCTGATTTATGTGTTAGAATAAATTCATGAAATTCGAT TCCAAGCATAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021964 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCGCTCGGCGCCC AACATGGCGGGTGGGCGCTGCGGCCCGCAGCTAACGGCGCTCCTGGCCGCCTGGATCGCG GCTGTGGCGGCGACGGCAGGCCCCGAGGAGGCCGCGCTGCCGCCGGAGCAGAGCCGGGTC CAGCCCATGACCGCCTCCAACTGGACGCTGGTGATGGAGGGCGAGTGGATGCTGAAATTT TACGCCCCATGGTGTCCATCCTGCCAGCAGACTGATTCAGAATGGGAGGCTTTTGCAAAG AATGGTGAAATACTTCAGATCAGTGTGGGGAAGGTAGATGTCATTCAAGAACCAGGTTTG AGTGGCCGCTTCTTTGTCACCACTCTCCCAGCATTTTTTCATGCAAAGGATGGGATATTC CGCCGTTATCGTGGCCCAGGAATCTTCGAAGACCTGCAGAATTATATCTTAGAGAAGAAA TGGCAATCAGTCGAGCCTCTGACTGGCTGGAAATCCCCGGCTTCTCTAACGATGTCTGGA ATGGCTGGTCTTTTTAGCATCTCTGGCAAGATATGGCATCTTCACAACTATTTCACAGTG ACTCTTGGAATTCCTGCTTTGGTGTTCTTATGTCTTTTTCGTATAGCCACCTTGGTTTTT GGCTTTTATGGGGTCTGTCTTGGGTGGAATATCAGATGTTTCTATGTGCCACTTCAGGCT TATCTTGGAGCGTTCTGACAAATTCGAATCAAGAGAGCTCATAACTGACAGTGCAGATGC GGGAGAGGAAAAGAGATTCATGGAGAAAACAAGACGCTTGNATGAAGAGAAAAAAANAAT CTGGCGTAGTTTAACAAGGAGAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC021964 unedited GGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGAATTTCATAAACCT GTACTCATTATTCTGTCTGGATCTAAGGAGCATAGAATCAACTTTAGGCATCTTTCGTAT GCTTGGAATCGAATTTCATGAATTTATTCTAACACATAAATCAGGTAAGAGAGAAGTAGA TGGATAGATTTTTTTTTTTTAAGGAAATTAGCCTCCCTGGGTACTTACAGTAGATTGAGT ATTGAGAGATTTACAAAGATATAATTCTTTGGAAGATTACAGAAGATGGAAACAAAATCA AATCTTTCACTTTCAGGTACTGGAGTTCATTAATTCTTTCACCAAAAGCACATCACTGAA GGAAAATCAGAAGTGGTTTTTTAGTTATTATTAAAGTAGTTCAAGACCCAGGGAACCCCT TGAGATGAAAACAAAACAGTATTCAACTTTTCTTCACAAGACTACCTTGTACTGGCAAGA CTTAGAGGACTTCTGGCTTGAAAAATATTGCTTAGAAAACTTAAAAAAAAATCAACAACA ACTATATTTTGGACAAAACAATTTTTTTTTAATCTGTCTTGTAAAATCCTTACTTCCTTT TGAGTCTCTGATGGCCACAACATTTCATTTGAGATGTTTGGCAGTCACAGCTTCAGGGGT TATGGTTACTGATTATCTAAACCCCTTAGGTCAGAATGAACAAACACAGTTCATGTAAAC CTGGCTGTTATTGGGAGTCCTCTGACCAAGAGATATAGTGAAGAGTGTGGAGAGCCCTGA TGCCCATGGTGGAAANTGCTGGAAAATTAAGGGTAGAAATAAACATAGCCAGGAGATTCG AGGGCTGGATGACTCTGAGCTGTTCTATCCCAGGCCTTNCATTCTAGCTGGAGATTTTAT GCCTGNGTGCCTGATGTCCTGCANTGACAAATAATACTACCAGAAAGATGCAGCTCTACN TACTTGGGAGCTAGAGCAGGTCTGCTGTCNATTNCA
Restriction Sites	NotI-NotI
ACCN	BC021964
Insert Size	2330 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021964.1
RefSeq Size	2418 bp
RefSeq ORF	1194 bp
Locus ID	56255
Protein Families	Druggable Genome, Transmembrane
Gene Summary	This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and C-terminal ASP/GLU-rich calcium binding domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The encoded protein has been shown to have reductase activity in vitro. [provided by RefSeq, Jan 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.