G protein beta subunit like (MLST8) (NM_022372) Human Untagged Clone

CAT#: SC127163

MLST8 (untagged)-Human MTOR associated protein, LST8 homolog (S. cerevisiae) (MLST8), transcript variant 1



  "NM_022372" in other vectors (6)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
MLST8 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GbetaL; GBL; LST8; POP3; WAT1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_022372, the custom clone sequence may differ by one or more nucleotides


ATGAACACCTCCCCAGGCACGGTGGGCAGTGACCCGGTCATCCTGGCCACTGCAGGCTACGACCACACCG
TGCGCTTCTGGCAGGCCCACAGCGGCATCTGCACCCGGACGGTGCAGCACCAGGACTCCCAGGTGAATGC
CTTGGAGGTCACACCGGACCGCAGCATGATTGCTGCTGCAGGTTACCAGCACATCCGCATGTATGATCTC
AACTCCAATAACCCTAACCCCATCATCAGCTACGACGGCGTCAACAAGAACATCGCGTCTGTGGGCTTCC
ACGAAGACGGCCGCTGGATGTACACGGGCGGCGAGGACTGCACAGCCAGGATCTGGGACCTCAGGTCCCG
GAACCTGCAGTGCCAGCGGATCTTCCAGGTGAACGCACCCATTAACTGCGTGTGCCTGCACCCCAACCAG
GCAGAGCTCATCGTGGGTGACCAGAGCGGGGCTATCCACATCTGGGACTTGAAAACAGACCACAACGAGC
AGCTGATCCCTGAGCCCGAGGTCTCCATCACGTCCGCCCACATCGATCCCGACGCCAGCTACATGGCAGC
TGTCAATAGCACCGGAAACTGCTATGTCTGGAATCTGACGGGGGGCATTGGTGACGAGGTGACCCAGCTC
ATCCCCAAGACTAAGATCCCTGCCCACACGCGCTACGCCCTGCAGTGTCGCTTCAGCCCCGACTCCACGC
TCCTCGCCACCTGCTCGGCTGATCAGACGTGCAAGATCTGGAGGACGTCCAACTTCTCCCTGATGACGGA
GCTGAGCATCAAGAGCGGCAACCCCGGGGAGTCCTCCCGCGGCTGGATGTGGGGCTGCGCCTTCTCGGGG
GACTCCCAGTACATCGTCACTGCTTCCTCGGACAACCTGGCCCGGCTCTGGTGTGTGGAGACTGGAGAGA
TCAAGAGAGAGTATGGCGGCCACCAGAAGGCTGTTGTCTGCCTGGCCTTCAATGACAGTGTGCTGGGCTA
G


>OriGene 5' read for NM_022372 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCCGTAAGAT
GCTCTGACCTTTGACCCTGCCGTTCAGCTCTAGGGCCCGTGCAGGCCACACCATGAACAC
CTCCCCAGGCACGGTGGGCAGTGACCCGGTCATCCTGGCCACTGCAGGCTACGACCACAC
CGTGCGCTTCTGGCAGGCCCACAGCGGCATCTGCACCCGGACGGTGCAGCACCAGGACTC
CCAGGTGAATGCCTTGGAGGTCACACCGGACCGCAGCATGATTGCTGCTGCAGGTTACCA
GCACATCCGCATGTATGATCTCAACTCCAATAACCCTAACCCCATCATCAGCTACGACGG
CGTCAACAAGAACATCGCGTCTGTGGGCTTCCACGAAGACGGCCGCTGGATGTACACGGG
CGGCGAGGACTGCACAGCCAGGATCTGGGACCTCAGGTCCCGGAACCTGCAGTGCCAGCG
GATCTTCCAGGTGAACGCACCCATTAACTGCGTGTGCCTGCACCCGAACCAGGCAGAGCT
CATCGTGGGTGACCAGAGCGGNGCTATCCACATCTGGGACTTGAAAACAGACCACAACGA
GCAGCTGATCCCTGAGCCCGAGGTCTCCATCACGTCCGCCCACATCGATCCCGACGCCAG
CTACATGGCAGCTGTCAATAGCACCGGAAACTGCTATGTCTGGAATCTGNACGGGGGCAT
TGGTGACGAGGTGACCCAGCTCATCCCCAAGACTAAGATCCCTGCCCACACGCGCTACGC
CCTGCAGTGTCGTTNCAGCCCCGACTTCACGCTCCTCGCCACCTGCTNGGGTGATCAGAC
GTGCCAGATCTGGNAGACGTCCAACTTCCTCCCTGATGACGGAGCTGAGCATCAAGAGCG
GCACCCCGGG
>OriGene 3' read for NM_022372 unedited
ACGTAAAAAGAGCTGCTTTTCATACTTTCCCAGATGCGTCCCCCGGACCTGGCCCAGCCT
GCCCCGTCCCCTTTCCATGGGCTGGCCAGGCCACCTGCACCAGGGAGGACAGCTGCTGGC
AGGGACTAATAAACCCTTCCACCTGGCCATGGTGGTGGTGTTCTCTATGGACCGAGGCCC
TGAAACGCGGGCAGGGAGGGGCAGAGAACGCACTAGCTTGGGGGTGGGCACCAGCCTCAG
ACCCCTCAGCAGCTTTGGGCCCTCGGCCGACTTTCCCAGGCAGTGCAGGCTAGCCCAGTC
CAGGAGTGTGCAGCCTGGCTTGGGTCGAGCTCTGTCACATCTGGATAAGCAACTGGGGGC
TGAGAGTCCCAGGGCAGCCTGGCCCAGCAGGTCAAGGCGCCACAGGGGGCCATCAGGTCC
AGCTGGCGCATGCCGGCAGGGGAGGGTCTGCTCTGACCTGAGTGCTGCATGGGTCCCTCC
AGCTGCCACCACCTGCACCAGGCAGTCCCGAGGGGTCACAGGCTAGCCCAGCACACTGTC
ATTGAAGGCCAGGCAGACAACAGCCTTCTGGTGGCCGCCATACTCTCTCTTGATCTCTCC
AGTCTCCACACACCAGAGCCGGGCCAGGTTGTCCGAGGAAGCAGTGACGATGTACTGNGA
GTCCCCCGAGAAGCGCAGCCCCACATTCAGCCGCGGGAAGACTTCCCCGGGATTGCCGCT
CTTGATGCTTACCTCCGTCATAAGGGAGAAGTTGGACGTCCTCCAGATCTTGCACGTTTG
ATCACCCCGACCAGGTGGCCAGGCACCGTGGAATCCGGCCTGAACCGACACTGCAGGGCG
TAACGCGTGTGGGCAGGGATTCTATTTTGGCGGATAACCTGGGTCACCTGTTACCATGCC
CCCCTTTAGATTCCAACATAGCAGCTTCCGGGCCTTTTGAAACCGCCT
Restriction Sites NotI-NotI     
ACCN NM_022372
Insert Size 1720 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022372.2, NP_071767.2
RefSeq Size 1931 bp
RefSeq ORF 984 bp
Locus ID 64223
UniProt ID Q9BVC4
Domains WD40
Protein Pathways mTOR signaling pathway
Gene Summary Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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