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CDw75 (ST6GAL1) (NM_173216) Human Untagged Clone
CAT＃: SC127214
ST6GAL1 (untagged)-Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SIAT1; ST6GalI; ST6N |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127214 sequence for NM_173216 edited (data generated by NextGen Sequencing)
ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTT GCAGTCATCTGTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTAAATTG CAAACCAAGGAATTCCAGGTGTTAAAGAGTCTGGGGAAATTGGCCATGGGGTCTGATTCC CAGTCTGTATCCTCAAGCAGCACCCAGGACCCCCACAGGGGCCGCCAGACCCTCGGCAGT CTCAGAGGCCTAGCCAAGGCCAAACCAGAGGCCTCCTTCCAGGTGTGGAACAAGGACAGC TCTTCCAAAAACCTTATCCCTAGGCTGCAAAAGATCTGGAAGAATTACCTAAGCATGAAC AAGTACAAAGTGTCCTACAAGGGGCCAGGACCAGGCATCAAGTTCAGTGCAGAGGCCCTG CGCTGCCACCTCCGGGACCATGTGAATGTATCCATGGTAGAGGTCACAGATTTTCCCTTC AATACCTCTGAATGGGAGGGTTATCTGCCCAAGGAGAGCATTAGGACCAAGGCTGGGCCT TGGGGCAGGTGTGCTGTTGTGTCGTCAGCGGGATCTCTGAAGTCCTCCCAACTAGGCAGA GAAATCGATGATCATGACGCAGTCCTGAGGTTTAATGGGGCACCCACAGCCAACTTCCAA CAAGATGTGGGCACAAAAACTACCATTCGCCTGATGAACTCTCAGTTGGTTACCACAGAG AAGCGCTTCCTCAAAGACAGTTTGTACAATGAAGGAATCCTAATTGTATGGGACCCATCT GTATACCACTCAGATATCCCAAAGTGGTACCAGAATCCGGATTATAATTTCTTTAACAAC TACAAGACTTATCGTAAGCTGCACCCCAATCAGCCCTTTTACATCCTCAAGCCCCAGATG CCTTGGGAGCTATGGGACATTCTTCAAGAAATCTCCCCAGAAGAGATTCAGCCAAACCCC CCATCCTCTGGGATGCTTGGTATCATCATCATGATGACGCTGTGTGACCAGGTGGATATT TATGAGTTCCTCCCATCCAAGCGCAAGACTGACGTGTGCTACTACTACCAGAAGTTCTTC GATAGTGCCTGCACGATGGGTGCCTACCACCCGCTGCTCTATGAGAAGAATTTGGTGAAG CATCTCAACCAGGGCACAGATGAGGACATCTACCTGCTTGGAAAAGCCACACTGCCTGGC TTCCGGACCATTCACTGCTAA Clone variation with respect to NM_173216.2 >OriGene 5' read for NM_173216 unedited
TAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGACCGACCGGCGTGGGCGCGGAGCGGGCGGCCGCCACCGAGCGTGCT GAGCAACCGCAGCCTCCGCGGCCGAGAGTGCAGCGAGCAAGGGGAGAGCCAGTTGCGCAG AGCCCTGCAACCAGCAGTCCAGGGAGAAGTGGTGAATGTCATGGAGCCCAGCTGAAATGG ACTGGCCCCCTTGAGCCTGTCCCAAGCCCTGGTGCCAGGTGTCCATCCCCGTGCTGAGAT GAGTTTTGATCATCCTGAGAAAAATGGGCCTTGGCCTGCAGACCCAATAAACCTTCCCTC CCATGGATAATAGTGCTAATTCCTGAGGACCTGAAGGGCCTGCCGCCCCTGNGGGATTAG CCAGAAGCAGGCTTGTTTTCCTGCTCAGAACAAAGTGACTTCCCTGAACACATCTTCATT ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTT GCAGTCATCTGTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTACATTG CAAACCAAGGAATTCCAGGTGTTAAAGAGTCTGGNGACATTGGCCATGGNGTCTGATTCC CAGTCTGTATNCTCAAGCAGCACCCAGAACCCCCACAGGGGCCGNCAGACCCTTCGCAGT CTCAGAGGCCTAGCCAAGCCAAACCAGAGGCCCTCTTCCAGGNTGTGGAACAGGNACAGC TCTTCCAAAACCTTATCCTAGCTGCAAAGATACTGGAGAATACCTAGCATGACCAGTCCA AGTGTCTACAAGGGCCAGACAGGCTCNAGTCAGTGCANAGCCCTGGCTGCACTCGACAAT GN >OriGene 3' read for NM_173216 unedited
CATCCAGTTGACCTATTGGGNGATGGTCAACTTCCCAGGNCCAGNAAAGCACTGGAGGAG GGTCACAGGGATGCCACCCGGGTTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATC TAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAAACACACACACT TTTATTTTAAAATTTGACATAATCCTTGGGTAATTATTAGCATTTTTCAAAAGTTTCCGA GACTGATATCTCAACTCCAAACCAATAGCAATAGCTTATTTGATTGGTGGAAGGATTAGA GAGGGTCCCATGATCCAGATGAATAGATGGCTATCAAAGAAGACAGAACCCCATAGACAC AGGCAGCATTAACTCATAATTTTTAAATTAACTGTCACAAATATGAAACATCTCACCAGA CCAATGATAACGTCCATATCATAATTTGCATTTTACCAAAGTGTCCACATAGACAGCACA GAACTAAAAGTTCTGTAGACTTCAGAATCATATTTAAATTAATTTGATCCTTTATATTAA TAATAATTAAAAACTAGATTCTACCCAGTGTCGTCCCAGTCTTCACACCAACATGTATGT CCTGCTGTCGGAATGCCACCTCCTCTGCACTCAGTGTCATGATTCGAAGATTAAAATCAG TGTGTCAAATCAGAACATAAACCAAGCCAGGAATTCATGGGCCCAGAGAGGGGTAAGGGG CTTGTCCTATACAGCAGTTAAGCAGCCCACGTGCTTCGCCCATTTTCCAAGCAAAATCAN ATTGGAGCTGCAGGCAGCGAGGAAGAAGGCGTGTTCTGTGTTGNCCACTGCTTTGCATGG TTTCTTGGGTGTTCCCAAGGAGACAGTTTGTGTCTCCCAATAGACGTTAATTCCCTGAGG ACGGACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_173216 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173216.1, NP_775323.1 |
| RefSeq Size | 4403 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 6480 |
| UniProt ID | P15907 |
| Protein Families | Secreted Protein |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2 and 4 encode the same protein (isoform a). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype
,Schultz, MJ;Holdbrooks, AT;Chakraborty, A;Grizzle, WE;Landen, CN;Buchsbaum, DJ;Conner, MB;Arend, RC;Yoon, KJ;Klug, C;Bullard, DC;Kesterson, RA;Oliver, PG;O'Connor, AK;Yoder, BK;Bellis, SL;,
Cancer Res.
,PubMed ID 27216178
[ST6GAL1]
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|
QUANTITY: An Isobaric Tag for Quantitative Glycomics
,Yang, S;Wang, M;Chen, L;Yin, B;Song, G;Turko, IV;Phinney, KW;Betenbaugh, MJ;Zhang, H;Li, S;,
Sci Rep
,PubMed ID 26616285
[ST6GAL1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203776 | ST6GAL1 (Myc-DDK-tagged)-Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1 |
CNY 5,488.00 |
|
| RC203776L1 | Lenti ORF clone of Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203776L2 | Lenti ORF clone of Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC203776L3 | Lenti ORF clone of Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203776L4 | Lenti ORF clone of Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG203776 | ST6GAL1 (tGFP-tagged) - Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1 |
CNY 7,088.00 |
