Methionyl tRNA synthetase (MARS) (AK092986) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMT2U; ILFS2; ILLD; METRS; MRS; MTRNS; SPG70
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092986, the custom clone sequence may differ by one or more nucleotides ATGACCTCACTGACTCACATTGACCACAGCTTGAGTCGTCAGAACTGTCCTTTCCTGGCTGGGGAGACAG AATCTCTAGCCGACATTGTTTTGTGGGGAGCCCTATACCCATTACTGCAAGATCCCGCCTACCTCCCTGA GGAGCTGAGTGCCCTGCACAGCTGGTTCCAGACACTGAGTACCCAGGAACCATGTCAGCGAGCTGCAGAG ACTGTACTGAAACAGCAAGGTGTCCTGGCTCTCCGGCCTCACCTCCAAAAGCAGCCCCAGCCCAGCCCCG CTGAGGGAAGGGCTGTCACCAATGAGCCTGAGGAGGAGGAGCTGGCTACCCTATCTGAGGAGGAGATTGC TATCTGCTGTACATTCGGCCTGAGGGCCAGGACAGTGCTTTCTCCTGGACGGACCTGCTGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092986 unedited AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGAAATGA GACTGTTCGTGAGTGATGGCGTCCCGGGTTGCTTGCCGGTGCTGGCCGCCGCCGGGAGAG CCCGGGGCAGAGCAGAGGTGCTCATCAGCACTGTAGGCCCGGAAGATTGTGTGGTCCCGT TCCTGACCCGGCCTAAGGTCCCTGTCTTGCAGCTGGATAGCGGCAACTACCTCTTCTCCA CTAGTGCAATCTGCCGATATTTTTTTTTGTTATCTGGCTGGGAGCAAGATGACCTCACTA ACCAGTGGCTGGAATGGGAAGCGACAGAGCTGCAGCCAGCTTTGTCTGCTGCCCTGTACT ATTTAGTGGTCCAAGGCAAGAAGGGGGAAGATGTTCTTGGTTCAGTGCGGAGAGCCCTGA CTCACATTGACCACAGCTTGAGTCGTCAGAACTGTCCTTTCCTGGCTGGGGAGACAGAAT CTCTAGCCGACATTGTTTTGTGGGGAGCCCTATACCCATTACTGCAAGATCCCGCCTACC TCCCTGAGGAGCTGAGTGCCCTGCACAGCTGGTTCCAGACACTGAGTACCCAGGAACCAT GTCAGCGAGCTGCAGAGACTGTACTGAAACAGCAAGGTGTCCTGGCTCTCCGGCCTTACC TCCAAAAGCAGCCCCAGCCCAGCCCCGCTGAGGGAAGGGCTGTCACCAATGAGCCTGAGG AGGAAGAGCTGGCTACCCTATCTGAGGAGGAGATTGCTATGGCCTGTACTGCTTGGGAGA AGGCCTANAAGTTTGCCCCGCTGCGGCCCCCACAAATCAGTGTTGCCTGTGGCTGGAGAA AGGAAGTGCTCATCACCAGGGCCCTCCCTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092986 unedited CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATTGTACATTTATTTATTACTG TCCCTATCTATTAAAGTGACTTTCTATGAGCCAAGGTCTTTTACTTTTTCTTCTTGCCTT TAAGGGCTTCAAGGGGTTTCCCCTCAGCTACAGCCAACTGTTTCTTTAGATCCAAGAGTT TCGCCACCTCCGCAGCAACCTCGTTCTTGTCTGCCTTTTGTGCTTTCAGTTCTCGGACAA TGTTTCCTTGTTTTGTCACTTCATCCATCAGCGCTTGTATCTGCTGTGGCTTGGCTGTTG TAACAGTCTCTACAACTGCTGGCTTCGGGGACGTTTTTGCCTGGCCCCCTCCAAAGCGCT GCCTTAAACTTTCAATCTGGTCATTTTCCAATTTTTGGAACAAGGGACTGACTGTGCCAA TCTGGTGTCCTGCTGGTAAAGTACACAGGAAGTTTGTCAGCAGGATACTGCACGCTGGAA GTGGGAGCTGCAGCTGGGCCTGGATTGTGGCACTAACCGTGGGCATGTAAGGCTGAAACA TGACAGAGAGCCAGGCCACCTTTTTCTCTGGCAAGCCATTCACTTGTCCTGCCCGTTGCC TTGTAGCCCCACTTGCCTTATTCGCTTCAAGGCCTATTCACCCGAATAATGGGTGCCTGG CCAGATTTGTAAGATACCGCCCAGCATCCCGGTTCCACCCTTTTAATTCTGGGGATTAGT TGGACCCCTGGTGCATGGCCAGCGGCCTTTTCTTAAGGGCGACTCCTCTCAGCCCTACCC CCAAAACTTTTACAACTTCCACTTCCTTACAATTTCCCACTTTATACCACTAAATTTTCT TTTACTGCGCGCCCCAATAATCCGTCCTGACCCTTGCCAACTCCATTATTAACCTCCATG TTTTGCTCCCGTCCTGACTATCCCAACTCCTCTCTTCCCTAAAATTCCTCCACTTTAN
Restriction Sites	NotI-NotI
ACCN	AK092986
Insert Size	2870 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092986.1, BAC04011.1
RefSeq Size	2695 bp
RefSeq ORF	414 bp
Locus ID	4141
Protein Families	Druggable Genome
Protein Pathways	Aminoacyl-tRNA biosynthesis, Selenoamino acid metabolism
Gene Summary	This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]

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