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CERK (NM_022766) Human Untagged Clone
CAT＃: SC127404
CERK (untagged)-Human ceramide kinase (CERK)
CNY 6,008.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dA59H18.2; dA59H18.3; hCERK; LK4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC127404 representing NM_022766.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGCGACGGGGGCGGCGGAGCCGCTGCAATCCGTGCTGTGGGTGAAGCAGCAGCGCTGCGCCGTG AGCCTGGAGCCCGCGCGGGCTCTGCTGCGCTGGTGGCGGAGCCCGGGGCCCGGAGCCGGCGCCCCCGGC GCGGATGCCTGCTCTGTGCCTGTATCTGAGATCATCGCCGTTGAGGAAACAGACGTTCACGGGAAACAT CAAGGCAGTGGAAAATGGCAGAAAATGGAAAAGCCTTACGCTTTTACAGTTCACTGTGTAAAGAGAGCA CGACGGCACCGCTGGAAGTGGGCGCAGGTGACTTTCTGGTGTCCAGAGGAGCAGCTGTGTCACTTGTGG CTGCAGACCCTGCGGGAGATGCTGGAGAAGCTGACGTCCAGACCAAAGCATTTACTGGTATTTATCAAC CCGTTTGGAGGAAAAGGACAAGGCAAGCGGATATATGAAAGAAAAGTGGCACCACTGTTCACCTTAGCC TCCATCACCACTGACATCATCGTTACTGAACATGCTAATCAGGCCAAGGAGACTCTGTATGAGATTAAC ATAGACAAATACGACGGCATCGTCTGTGTCGGCGGAGATGGTATGTTCAGCGAGGTGCTGCACGGTCTG ATTGGGAGGACGCAGAGGAGCGCCGGGGTCGACCAGAACCACCCCCGGGCTGTGCTGGTCCCCAGTAGC CTCCGGATTGGAATCATTCCCGCAGGGTCAACGGACTGCGTGTGTTACTCCACCGTGGGCACCAGCGAC GCAGAAACCTCGGCGCTGCATATCGTTGTTGGGGACTCGCTGGCCATGGATGTGTCCTCAGTCCACCAC AACAGCACACTCCTTCGCTACTCCGTGTCCCTGCTGGGCTACGGCTTCTACGGGGACATCATCAAGGAC AGTGAGAAGAAACGGTGGTTGGGTCTTGCCAGATACGACTTTTCAGGTTTAAAGACCTTCCTCTCCCAC CACTGCTATGAAGGGACAGTGTCCTTCCTCCCTGCACAACACACGGTGGGATCTCCAAGGGATAGGAAG CCCTGCCGGGCAGGATGCTTTGTTTGCAGGCAAAGCAAGCAGCAGCTGGAGGAGGAGCAGAAGAAAGCA CTGTATGGTTTGGAAGCTGCGGAGGACGTGGAGGAGTGGCAAGTCGTCTGTGGGAAGTTTCTGGCCATC AATGCCACAAACATGTCCTGTGCTTGTCGCCGGAGCCCCAGGGGCCTCTCCCCGGCTGCCCACTTGGGA GACGGGTCTTCTGACCTCATCCTCATCCGGAAATGCTCCAGGTTCAATTTTCTGAGATTTCTCATCAGG CACACCAACCAGCAGGACCAGTTTGACTTCACTTTTGTTGAAGTTTATCGCGTCAAGAAATTCCAGTTT ACGTCGAAGCACATGGAGGATGAGGACAGCGACCTCAAGGAGGGGGGGAAGAAGCGCTTTGGGCACATT TGCAGCAGCCACCCCTCCTGCTGCTGCACCGTCTCCAACAGCTCCTGGAACTGCGACGGGGAGGTCCTG CACAGCCCTGCCATCGAGGTCAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCACGAGGAATTGAAGAG AATCCGAAGCCAGACTCACACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022766 |
| Insert Size | 1614 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022766.4 |
| RefSeq Size | 4445 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 64781 |
| UniProt ID | Q8TCT0 |
| Domains | DAGKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 60 kDa |
| Gene Summary | CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213995 | CERK (Myc-DDK-tagged)-Human ceramide kinase (CERK) |
CNY 6,000.00 |
|
| RC213995L3 | Lenti-ORF clone of CERK (Myc-DDK-tagged)-Human ceramide kinase (CERK) |
CNY 5,890.00 |
|
| RC213995L4 | Lenti-ORF clone of CERK (mGFP-tagged)-Human ceramide kinase (CERK) |
CNY 5,890.00 |
|
| RG213995 | CERK (tGFP-tagged) - Human ceramide kinase (CERK) |
CNY 7,600.00 |
