SLC35B4 (AK056475) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	YEA; YEA4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056475, the custom clone sequence may differ by one or more nucleotides ATCTTGAATAGGAGGAACGCTGTTGCTGTAATATGCGTAGCTTGTGTGTGCTCTTGTGTAGTCTGTGTGT GATCAAAGGGCACGGAAGTGGCTGCACTTTCACATCCCTTTTCTTTACTTAGCTGGAACCTTACATACCA GTACATGCTCCTCTGTAAGCAGCTGAACTCACCGGCCACTTCTCTGTTTGCCTATAGACAAAGACTGTGT GTGTGTGTGTGTGTGTGTATGTATGTGTGTGTGTGGTGCACACATTGTGTTCTTAAACTGAGACGTGGCT CTGCAGGTCTCCTGGGCTCATTCCATGGTGTGGTATGTTTATTCCACTGTCCAGAGCTATTCTCTGATGG ATTTGAGCAACAGCAGTGGAGATAAATGTCCTAGAGTCACCAGGTCGCTTGGAGAAGTCATTTAAGCTGC CTCGGGTTTTTGTTACTTAAAATGTGGATATTATTTCTCCACCTAAATCACTGAGTTTACAGAGTAATAA TGTGTTGCTCTGGACATTGACAGCTTTCTAGAGCCAGTAATGGGCTCTTCTGAAGGATGCTGAATTAGAA GTGAACCTATTCATAGGATCAAAAGCCACTTGCTTTGAAATATGTAGAGTTCCTCAGAATTGACGGTGCT AGAAATATCCAAGTGTTAAATAACCCTTTAAAAGCAACAAAAGCTACTTTTTTCTTACCACTTAATAGAA GAACCTGTCCCTAGAGGCGACTTCATTGCTATGGATCTGGAGTCTCTGAACTCTTAATAGGATGCAGCCT CACATACATAATGTCACCCATTTTATGTTGATGAAAACATTACAAGTTTTCATCATTGGGTATGTGTTGA TGTTCACAGACAGTACTTGGGCCCATTAGGTTTTCGCGTCTGGTCTTAGAGCATGTGTATTCATCTCAGC GTGAATACCTCAGCCAGTCTTATGAATAGGAGGCTATGTATGGGCAGGGAATAGATTAGATTAGAACACT CGCACAAGATGACCACATATAGAAGCAGAAAGCCCCAAATTCCCTGGGAACTGTTTTTTCTCTAGTAGCT GAATATAAGGAAATATGTCTAATGGACACCAGTTAATACTTTTTAAAACTACTCTTTAAAAAAAAAATAC GTTCCCCTTGGTTAACTGATTTTTTAATCCAGGGTGGACATTTTTTCAACCTTTATTAAAAAGACAAATA AACTATTTTGTAGAAGATCAGACTCCTACTTAACTGGAAGAGAAATGTCTATTAAATGTCTCTCCTCTTT CTCTGGGTCAAGACCATGTAATTTTATGCTTCAGAGATGAAGATACTGTTTGTTTACAAAGAGTTTAGTT TTTAAGACATCCAAAACTCTATGCTAGAGCAAAAATCAAATAGCAAAGGACACTAGCCAGAAAATACAGT GTGTGTGTGTGCACCTGTGTGCCTGCTGAACAACTTGACAGTGTAACAGATAAGGTAACTGAAGATGGTG GATATTTGAATTGTATTAGCTTAATGTCTACATATCTTTGGCCAAAACTCTATTGTCATATTAGAAACAT GTTATCTTTTTCATGTTTATTAGTAATTTATTTTTGATTCTTTGTTTTCTTTTTCGTCCAACTAAAACAA CTGTAATGTACTTGATACATTTATATCAAGTTCTAAAGTATTTAGACAAATCCAAATACTTTGTTTTTAG TTTTTTCCTCCTTTCCATCCTGTTAACCACAGTGAAACGCTGCAGTATTTTGATTTGGTCAGTGCTACGG AGGAAGACCATGAAAGCTGAATTGGTCTGTGCCACCCAGAGTAAACCTCTTCTCTTCTTCTGGAAAGATG GCGTGATGTTTTTCAAGGATTCTAATAAATATCCCGCAGTCATCTCCTGAGCAGCTTTCCTCTGTTTCAC TTCGGAACTCCTTCCCCCACTTTCTTCACTCCAGTTTCTTCACTTGCAGCGTTTTCTGCTAAGAAAGACT TATCATCTTGTATCTGTTTATGCCTCTCTTCTTTGGGGCCCCTGGATGTTTTTGTGGATTTTTACAGAGT ATTTATATCTGCACAGCGTGTATTTTGTTATACATTCTTACAAGCATTGATATTGGGGGGTACATTTATA ATAGTGAGAATATCTGGCAGAATGTGTGAATGCCATATCTGTAGATGACGATGAATGTGAAGGGTTAATG TAGAAATGTTTGTTTATTCTGTTACAGCTCAAAATGGAGGCTGAGAATCTCTACCACCATTCCCTTCTCT ATTTGAGAATCTCTACCACCATTCCCTTCTCTATTTGGGAAACAATACTCTCTTATTTTGCTATTTTTAC CTTCCCATTAACTGGCTTCACTCAACAGTGCCAGATACCTATGGAGAGGGGAAGTAAAACACCTTAGGTC AGGAATAATAAACCAATGAGTTAGCTCAGGGCCGGTGGACATGTTCACTGGTGGACCAGTTGGCATTGAC CTAAACGGACAGAGAAATGCACATGGAGGAGAACTACGTTGAAATCAAGATCCTATGTCTGTTGTATTTT CTCTTTGCCTGGAGTAAATAGCATTATAAGTCATTCCACCCTTATTAGATAACCAAAGCATATTGGTCTC AATAGCTTTCTCCTGAAAAATGTGATTGAATCTTACCTGTAGCAGTTCACTCTTAAGGTTGAAAGAACTG TCAATATAGATAAAATCCATTCCTTATTATTATTGATTGGTGTGACATTTGAAAATGTGTCTAAACAATA TTTGTAAGTAAAAGAACCAGTCATAAGCCTCAAATGTGTTCAGTGTGTACCAAGCTGGAATGGGAGACTG GCCCCTCTTGGCTTTAAAGTAGCATGTTGTCTTTTTCTAGCAGCTTCTTCCTAGTAGACATTCCAGGGAT GGGAGGGGCCAGGGATCATGTCTTACTCATTTCTGTATCGCCAGCCCCTGGCATAGTGTCTGGCTTATGT TACGTGAATAAATGAATGCTTGCC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056475 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGAGCAAAAATCAAAT AGCAAAGGACACTAGCCAGAAAATACAGTGTGTGTGTGTGCACCTGTGTGCCTGCTGAAC AACTTGACAGTGTAACAGATAAGGTAACTGAAGATGGTGGATATTTGAATTGTATTAGCT TAATGTCTACATATCTTTGGCCAAAACTCTATTGTCATATTAGAAACATGTTATCTTTTT CATGTTTATTAGTAATTTATTTTTGATTCTTTGTTTTCTTTTTCGTCCAACTAAAACAAC TGTAATGTACTTGATACATTTATATCAAGTTCTAAAGTATTTAGACAAATCCAAATACTT TGTTTTTAGTTTTTTCCTCCTTTCCATCCTGTTAACCACAGTGAAACGCTGCAGTATTTT GATTTGGTCAGTGCTACGGAGGAAGACCATGAAAGCTGAATTGGTCTGGTGCCACCCAGA GTAAACCTCTTCTCTTCTTCTGGAAAGATGGCGTGATGTTTTTCAAGGATTCTAATAAAT ATCCCGCAGTCATCTCCTGAGCAGCTTTCCTCTGTTTCACTTCGGAACTCCTTCCCCCAC TTTCTTCACTCCAGTTTCTTCACTTGCAGCGTTNTCTGCTAAGAAAGACTTATCATCTTG TATCTGTTTATGCCTCTCTTCTTTTGGGCCCCTGGATGNNTTTGTGGATTTTTACAGAGT ATTTATATCTGCACAGCGTGTATTTTGTTATACATTCNTACCAGCATTGATATTGGGGGG GTACATTCATTATAGTGAGAATATCTGGCAGAATGTGTGAATGCCATATCTGTAAATGAC GATGAAATGTGAAGGGTTATGTANAAATTGTTTGTTTATTCTGTTACAGCTCAAAATGGA GCT
Restriction Sites	NotI-NotI
ACCN	AK056475
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056475.1
RefSeq Size	2964 bp
RefSeq ORF	2964 bp
Locus ID	84912
Protein Families	Transmembrane
Gene Summary	Glycosyltransferases, such as SLC35B4, transport nucleotide sugars from the cytoplasm where they are synthesized, to the Golgi apparatus where they are utilized in the synthesis of glycoproteins, glycolipids, and proteoglycans (Ashikov et al., 2005 [PubMed 15911612]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.