RPL29 (AF116627) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF116627, the custom clone sequence may differ by one or more nucleotides ATGCCCCGGAAGATCACCGAGGTGCTAGTACGAAAGGTCCCTGACAACCAACAGTTCCTAGACCTCCGTG TGGCCGTCCTGGGGAATGTGGACTCTGGGAAGTCAACTCTGCTTGGAGTCCTGACCCAGGGAGAGCTGGA CAATGGGCGGGGCCGGGCTCGGCTCAACCTTTTCCGCCACCTGCATGAGATTCAGTCTGGCCGAACCTCC AGCATCAGCTTCGAGATCCTGGGCTTTAACAGCAAGGGAGAGGTGCATGGGATCAATGGGACCCAATGGG GCCAGACTCTGAGGATGGGATGGTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF116627 unedited GCACGAGGGCGCTTCGGGAGCCGCGGCTTATGGTGCAGACATGGCCAAGTCCAAGAACCA CACCACACACAACCAGTCCCGAAAATGGCACAGAAATGGTATCAAGAAACCCCGATCACA AAGATACGAATCTCTTAAGGGGGTGGACCCCAAGTTCCTGAGGAACATGCGCTTTGCCAA GAAGCACAACAAAAAGGGCCTAAAGAAGATGCAGGCCAACAATGCCAAGGCCATGAGTGC ACGTGCCGAGGCTATCAAGGCCCTCGTAAAGCCCAAGGAGGTTAAGCCCAAGATCCCAAA GGGTGTCAGCCGCAAGCTCGATCGACTTGCCTACATTGCCCACCCCAAGCTTGGGAAGCG TGCTCGTGCCCGTATTGCCAAGGGGCTCAGGCTGTGCCGGCCAAAGGCCAAGGCCAAGGC CAAGGCCAAGGATCAAACCAAGGCCCAGGCTGCAGCCCCAGCTTCAGTTCCAGCTCAGGC TCCCAAACGTACCCAGGCCCCTACAAAGGCTTCAGAGTAGATATCTCTGCCAACATGAGG ACAGAAGGACTGGTGCGACCCCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF116627 unedited CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTCCCGGCCTAAGGTTTATTTGTACAAATAGCACAGGA GGACCCCACCCCCATGCAAATGGTACCCCAGGGGCGGGGGGGGGGGGTCAAACCAATCCT TTTGTCTTAATGTTGGAAAAAATATTTACTTTAAACCCTTTGAAGGGCCCTGGGTACTTT TGGAACCCTAACCTGAAACTAAACCTGGGGCTGAACCCTGGCCCTTGGTTAAATCCTTGG CCTTGGCCTTGGCCTTGGCCTTTGGCCGGAACAACCTGACCCCTTTGGAAAAACGGGCAA AAACACCCTTCCAAAGTTTGGGGGGGGCAATGTAGGCAAGTCAATCAACTTTGGGGTTAA CACCCTTTGGAATCTTGGGCTAAACCTCCTTGGGCTTAACAAGGGCCTTAATAACTTTGG CACGGGCACTCATGGCCTTGGAATTGTGGGCCCCGTATTTTTTTAGGCCCTTTTTGTTGC CCTTCTTGGAAAGACCCATTGTCTCTAAAGAACTTGGGGCCCCCCCCCTTAAGAAAATCC ATTTTTTGGAACCAGGGTTTTTTGAAACCATTTTTGGGCCATTTTAGGGACTGGTTGGGT GCGGGGGGGTCTTTGAACTTGGCCACTTTTCACAATAAAACCCGGGTTCCCAAAACGCCT TTTGCCAAATTCGGGGCCCCCTAAATGAATTCCTATAAAAAATTTGAAAGGTCAAAAAAA AAAATTTTTTATTATAAACATTCAATGTAAACCCTTTATGCCATTTTGGTAAACGGGGGC GGGTATTAAAAACATTGGGAAAACTCCCTTATTTTGGCCCAAAAAAAATCCTCATTGTTA AAGTGGTGGAAAACTTGAAACCCGGTGAACAAACCTTTCCCCCCTTTTGGTC
Restriction Sites	NotI-NotI
ACCN	AF116627
Insert Size	750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF116627.1, AAF71050.1
RefSeq Size	1487 bp
RefSeq ORF	1487 bp
Locus ID	6159
Domains	Ribosomal_L29e
Protein Pathways	Ribosome
Gene Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

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