United States
Germany
Japan
United Kingdom
China
AF9 (MLLT3) (NM_004529) Human Untagged Clone
CAT＃: SC127943
MLLT3 (untagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3)
CNY 4,240.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AF9; YEATS3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127943 sequence for NM_004529 edited (data generated by NextGen Sequencing)
ATGGCTAGCTCGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGG AAAAAACCCACCGTGGAGGGCTTCACCCACGACTGGATGGTGTTCGTACGCGGTCCGGAG CACAGTAACATACAGCACTTTGTGGAGAAAGTCGTCTTCCACTTGCACGAAAGCTTTCCT AGGCCAAAAAGAGTGTGCAAAGATCCACCTTACAAAGTAGAAGAATCTGGGTATGCTGGT TTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGAAGAACCTAGGAAAGTCCGCTTT GATTATGACTTATTCCTGCATCTTGAAGGCCATCCACCAGTGAATCACCTCCGCTGTGAA AAGCTAACTTTCAACAACCCCACAGAGGACTTTAGGAGAAAGTTGCTGAAGGCAGGAGGG GACCCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGC AGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGC AGCAGTAGCAGCAGCAGCAGCAGCAGTAGTACCAGTTTTTCAAAGCCTCACAAATTAATG AAGGAGCACAAGGAAAAACCTTCTAAAGACTCCAGAGAACATAAAAGTGCCTTCAAAGAA CCTTCCAGGGATCACAACAAATCTTCCAAAGAATCCTCTAAGAAACCCAAAGAAAATAAA CCACTGAAAGAAGAGAAAATAGTTCCTAAGATGGCCTTCAAGGAACCTAAACCCATGTCA AAAGAGCCAAAACCAGATAGTAACTTACTCACCATCACCAGTGGACAAGATAAGAAGGCT CCTAGTAAAAGGCCGCCCATTTCAGATTCTGAAGAACTCTCAGCCAAAAAAAGGAAAAAG AGTAGCTCAGAGGCTTTATTTAAAAGTTTTTCTAGCGCACCACCACTGATACTCACTTGT TCTGCTGACAAAAAACAGATAAAAGATAAATCTCATGTCAAGATGGGAAAGGTCAAAATT GAAAGTGAGACATCAGAGAAGAAGAAATCAACGTTACCGCCATTTGATGATATTGTGGAT CCCAATGATTCAGATGTGGAGGAGAATATATCCTCTAAATCTGATTCTGAACAACCCAGT CCTGCCAGCTCCAGCTCCAGCTCCAGCTCCAGCTTCACACCATCCCAGACCAGGCAACAA GGTCCTTTGAGGTCTATAATGAAAGATCTGCATTCTGATGACAATGAGGAGGAATCAGAT GAAGTGGAGGATAACGACAATGACTCTGAAATGGAGAGGCCTGTAAATAGAGGAGGCAGC CGAAGTCGCAGAGTTAGCTTAAGTGATGGCAGCGATAGTGAAAGCAGTTCTGCTTCTTCA CCCCTACATCACGAACCTCCACCACCCTTACTAAAAACCAACAACAACCAGATTCTTGAA GTGAAAAGTCCAATAAAGCAAAGCAAATCAGATAAGCAAATAAAGAATGGTGAATGTGAC AAGGCATACCTAGATGAACTGGTAGAGCTTCACAGAAGGTTAATGACATTGAGAGAAAGA CACATTCTGCAGCAGATCGTGAACCTTATAGAAGAAACTGGACACTTTCATATCACAAAC ACAACATTTGATTTTGATCTTTGCTCGCTGGACAAAACCACAGTCCGTAAACTACAGAGT TACCTGGAAACATCTGGAACATCCTGA Clone variation with respect to NM_004529.2 >OriGene 5' read for NM_004529 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCATGCTCCGCAAT CATCTTCTTTACCCTGGAGCTGCTGCTGCTGCTGCTGCTTTTGCTTTTGGGGCTGAGTTT AATAAGCGAGCGAGCGAGCAAGCGAGCGCGGGGGGAAAAAGGCAGAGAATGTCCGCCATC TACCCTCCGCTCCTGGGCGCGCTCTCATTCATAGCAGCCTCTTCATGAATTACAGCTGAG GGGGGGCGGAGGAGGGGGGGGTACCACACAACACCCCAGCAAACCTCCGGGCCCCCAGGC ATGGCTAGCTCGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGG AAAAAACCCACCGTGGAGGGCTTCACCCACGACTGGATGGTGTTCGTACGCGGTCCGGAG CACAGTAACATACAGCACTTTGTGGAGAAAGTCGTCTTCCACTTGCACGAAGCTTCCTAG CCAAAGAGGGCAAGATCACCTTACAAGAAAAATTGGGATGCGGTATTTGCATGAATTATT TAAAACAGAAAACCTAGAACCGTTGAATGACTTATCGCTCTGAGCCTCACCGGAATACCT CGTGGAAAGTACTTCACACCCACGAGACTTAGGAGAAGTGCTGAGGCANGAGGGGGACCC TATAAGAGTATNCATACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGC AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGT AGCAGCAGCAGCAGCAGCAGTAGTACCAGTTTTTTCAAGGCCTACAATNAATGAAAGGAG CCA >OriGene 3' read for NM_004529 unedited
TTCAAAACAGGGTACAACAAGAAACAAAAAAATCCCCCCATCCCCAACCGTCTTTCACCA ATACAAGAAATCCTTCTGGAAATATTAGCAAGCAACTAAACAGGTATTGAACCACTGAAT CTACAAGTTAATACATAATCTGTTATGGCAGAATAACCAAGAATTTTTCCCCAAAGAAAG ATTTTAATTTTTTCAAATATAAAAGTGTCTAAGAGAATCATATGTGAACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004529 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004529.1, NP_004520.1 |
| RefSeq Size | 3376 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 4300 |
| UniProt ID | P42568 |
| Domains | YEATS |
| Protein Families | Transcription Factors |
| Gene Summary | Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948, PubMed:25417107, PubMed:27105114, PubMed:27545619). Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated (PubMed:25417107, PubMed:27105114, PubMed:27545619). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25417107, PubMed:27105114, PubMed:27545619). Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3 (PubMed:25417107, PubMed:27105114). In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones (PubMed:27105114, PubMed:27545619).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206283 | MLLT3 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3) |
CNY 4,232.00 |
|
| RC206283L1 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), Myc-DDK-tagged |
CNY 6,632.00 |
|
| RC206283L2 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), mGFP tagged |
CNY 5,890.00 |
|
| RC206283L3 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206283L4 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), mGFP tagged |
CNY 6,632.00 |
|
| RG206283 | MLLT3 (tGFP-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3) |
CNY 5,832.00 |
