SEMA4A (NM_022367) Human Untagged Clone

CAT#: SC128157

SEMA4A (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A), transcript variant 1



  "NM_022367" in other vectors (7)

CNY 5,584.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
SEMA4A Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CORD10; RP35; SEMAB; SEMB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC128157 sequence for NM_022367 edited (data generated by NextGen Sequencing)
ATGGCCCTCCCAGCCCTGGGCCTGGACCCCTGGAGCCTCCTGGGCCTTTTCCTCTTCCAA
CTGCTTCAGCTGCTGCTGCCGACGACGACCGCGGGGGGAGGCGGGCAGGGGCCCATGCCC
AGGGTCAGATACTATGCAGGGGATGAACGTAGGGCACTTAGCTTCTTCCACCAGAAGGGC
CTCCAGGATTTTGACACTCTGCTCCTGAGTGGTGATGGAAATACTCTCTACGTGGGGGCT
CGAGAAGCCATTCTGGCCTTGGATATCCAGGATCCAGGGGTCCCCAGGCTAAAGAACATG
ATACCGTGGCCAGCCAGTGACAGAAAAAAGAGTGAATGTGCCTTTAAGAAGAAGAGCAAT
GAGACACAGTGTTTCAACTTCATCCGTGTCCTGGTTTCTTACAATGTCACCCATCTCTAC
ACCTGCGGCACCTTCGCCTTCAGCCCTGCTTGTACCTTCATTGAACTTCAAGATTCCTAC
CTGTTGCCCATCTCGGAGGACAAGGTCATGGAGGGAAAAGGCCAAAGCCCCTTTGACCCC
GCTCACAAGCATACGGCTGTCTTGGTGGATGGGATGCTCTATTCTGGTACTATGAACAAC
TTCCTGGGCAGTGAGCCCATCCTGATGCGCACACTGGGATCCCAGCCTGTCCTCAAGACC
GACAACTTCCTCCGCTGGCTGCATCATGACGCCTCCTTTGTGGCAGCCATCCCTTCGACC
CAGGTCGTCTACTTCTTCTTCGAGGAGACAGCCAGCGAGTTTGACTTCTTTGAGAGGCTC
CACACATCGCGGGTGGCTAGAGTCTGCAAGAATGACGTGGGCGGCGAAAAGCTGCTGCAG
AAGAAGTGGACCACCTTCCTGAAGGCCCAGCTGCTCTGCACCCAGCCGGGGCAGCTGCCC
TTCAACGTCATCCGCCACGCGGTCCTGCTCCCCGCCGATTCTCCCACAGCTCCCCACATC
TACGCAGTCTTCACCTCCCAGTGGCAGGTTGGCGGGACCAGGAGCTCTGCGGTTTGTGCC
TTCTCTCTCTTGGACATTGAACGTGTCTTTAAGGGGAAATACAAAGAGTTGAACAAAGAA
ACTTCACGCTGGACTACTTATAGGGGCCCTGAGACCAACCCCCGGCCAGGCAGTTGCTCA
GTGGGCCCCTCCTCTGATAAGGCCCTGACCTTCATGAAGGACCATTTCCTGATGGATGAG
CAAGTGGTGGGGACGCCCCTGCTGGTGAAATCTGGCGTGGAGTATACACGGCTTGCAGTG
GAGACAGCCCAGGGCCTTGATGGGCACAGCCATCTTGTCATGTACCTGGGAACCACCACA
GGGTCGCTCCACAAGGCTGTGGTAAGTGGGGACAGCAGTGCTCATCTGGTGGAAGAGATT
CAGCTGTTCCCTGACCCTGAACCTGTTCGCAACCTGCAGCTGGCCCCCACCCAGGGTGCA
GTGTTTGTAGGCTTCTCAGGAGGTGTCTGGAGGGTGCCCCGAGCCAACTGTAGTGTCTAT
GAGAGCTGTGTGGACTGTGTCCTTGCCCGGGACCCCCACTGTGCCTGGGACCCTGAGTCC
CGAACCTGTTGCCTCCTGTCTGCCCCCAACCTGAACTCCTGGAAGCAGGACATGGAGCGG
GGGAACCCAGAGTGGGCATGTGCCAGTGGCCCCATGAGCAGGAGCCTTCGGCCTCAGAGC
CGCCCGCAAATCATTAAAGAAGTCCTGGCTGTCCCTAACTCCATCCTGGAGCTCCCCTGC
CCCCACCTGTCAGCCTTGGCCTCTTATTATTGGAGTCATGGCCCAGCAGCAGTCCCAGAA
GCCTCTTCCACTGTCTACAATGGCTCCCTCTTGCTGATAGTGCAGGATGGAGTTGGGGGT
CTCTACCAGTGCTGGGCAACTGAGAATGGCTTTTCATACCCTGTGATCTCCTACTGGGTG
GACAGCCAGGACCAGACCCTGGCCCTGGATCCTGAACTGGCAGGCATCCCCCGGGAGCAT
GTGAAGGTCCCGTTGACCAGGGTCAGTGGTGGGGCCGCCCTGGCTGCCCAGCAGTCCTAC
TGGCCCCACTTTGTCACTGTCACTGTCCTCTTTGCCTTAGTGCTTTCAGGAGCCCTCATC
ATCCTCGTGGCCTCCCCATTGAGAGCACTCCGGGCTCGGGGCAAGGTTCAGGGCTGTGAG
ACCCTGCGCCCTGGGGAGAAGGCCCCGTTAAGCAGAGAGCAACACCTCCAGTCTCCCAAG
GAATGCAGGACCTCTGCCAGTGATGTGGACGCTGACAACAACTGCCTAGGCACTGAGGTA
GCTTAA

Clone variation with respect to NM_022367.3
1716 c=>t
>OriGene 5' read for NM_022367 unedited
TGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCTGGCTTTGGCATGATGGG
CACCTGGAGGGCCGCACTCCCGTTCCAGCCAGGCTGAGCCTTCTGTCCCCTGCCTCTGGG
GCCTGGGAACCCCCCTTCTTCTTTCTCCTGAATGGCACCCCCGCCCTAGAATCCAGACAC
CGAGTTTCCCACTGTGGCTGGTTCAAGGAGCTCCCTGGTGACAGTCTGTGGCTGAGCATG
GCCCTCCCAGCCCTGGGCCTGGACCCCTGGAGCCTTCTGGGCCTTTTCCTCTCCCAACTG
CTTCAGCTGCTGCTGCCGACGACCACCGCGGGGGGAGGCGGGCAGGGGCCCATGCCCAGG
GCCATATACTATGCAGGGGATGAACGTACGGCACTTAGCTTCTTCCACCAGAAGGGCCTC
CAGGATTTTGACACTCTGCTCCTGAGTGGNGACGGAAATACTCTCTACGTGGGGGCTCGA
GAAGCCATTCTGGCCTTGGATATCCCCCATCCCAGGGGTCCCCAGGCTAAAGAACATGAT
ACCGTGGCCAGCCCGTGACCGAAAAAAGAGCGAATGCGCCTTTAACACGAAAGCAATGAG
AACACAGGTTTCACTCCATCCGCGTCCTGGCTTTCTTACAATGTCACCCATCTCTACACC
CTGCGGAACCTTCCCCCTTAACCCTGCTTGCCCCCTTCTTGAAATTCCCGACTCCTACCT
GGTGCCCACCTTGCAGACAATGTCACTGCGGCGAAAAGCCCAAACCCCTTTGCCCCGTTC
CCAACCTACGGCTTGTCTCGTGGAGTGCGAGGCCCTTTTTGGGATACGAAAAATTCCTTG
GCAGTGCGCCCTTCTGATGCCACACTGGGACCCACTCTGTCCTAAACCGCCACCTTCTCC
CTGGCTCGATATGAACCCCCTTGGGGCAGACTCCCT
>OriGene 3' read for NM_022367 unedited
TANGATCCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCTTATATTTTT
AATAAAAAATAAACAGTCTCTGACAAGCAGTTTTCTGAATCCCAAAACAAAGGAAAAGGG
AGGGGGAGAGGTGAAGGGGTCAGCTAGGGTAAAGGAGTGAAGAAGGCTCAGATTACCCCT
GCCATTCTGCCAGGGCAGAAGGGATCAGAGTCTGCCCCAACTGAAGCAAGAAGAAAGGTG
GTCAGACTTCAGGGAAGACTTCCTGGGAGTCAGCGGTGCACGACTGGTAAGGGAAGCAGG
AGGGAGCAGATCCCTGCATGACCCTGGGAGAAGGGAGTGTTGGTGTCCAAAGCGGCAGCT
TCAGAGTGGAGTTTCCAGGAGTGGCATGTTAGCATATGATTGTTTAGATGTTTGGTGTTC
ATTACCATAGGGTCCTGGGACAGGCAGGTTTTTTAGGGTCTCTTGAAACACTGTGTTTCT
GGAGGGTCCCTGGAATGGCCAGAACTGAAGGATCCTCTCCAGGTTCTTCAATATCAGTGT
AGGAGCAGGTCTGGGGGTAGCCCCCACCCTGTTAGAGAGCTCATGTGCTTGGTAGAAGGG
AGTCCCATAGGGGAGGCAGACTGCTGTGCATCACCCTGCTGAGTGTCATCAGTGATGCAG
AGTAGCAGAAGCTCCTCTCAGGGGAGAAAGGTGGTCTTTTGTGCTGCTGTCATCCTAGTC
AGGGCTGGGCTTGGCCCGCCCAGCCAGCATGGCCAGGTGCCTGGACCGGAACCCCGGCCT
GGGCCTAGAAGTTTAGCTACCCCAGTGCCTAAGCCGTTGGTGTCAGCGTCCACATCACTG
GCAGAAGTCCTGGCATCTTTGGAGACTGGG
Restriction Sites ECoRI-NOT     
ACCN NM_022367
Insert Size 3900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022367.2, NP_071762.2
RefSeq Size 3257 bp
RefSeq ORF 2286 bp
Locus ID 64218
UniProt ID Q9H3S1
Domains Sema, PSI, PSI
Protein Families Transmembrane
Protein Pathways Axon guidance
Gene Summary This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]
Transcript Variant: This variant (1) is the longest transcript. Variants 1-3 encode the same isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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