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SMN1 (NM_000344) Human Untagged Clone
CAT＃: SC128237
SMN1 (untagged)-Human survival of motor neuron 1, telomeric (SMN1), transcript variant d
CNY 2,400.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCD541; GEMIN1; SMA; SMA1; SMA2; SMA3; SMA4; SMA@; SMN; SMNT; T-BCD541; TDRD16A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000344 edited
GGCCCCACGCTGCGCACCCGCGGGTTTGCTATGGCGATGAGCAGCGGCGGCAGTGGTGGC GGCGTCCCGGAGCAGGAGGATTCCGTGCTGTTCCGGCGCGGCACAGGCCAGAGCGATGAT TCTGACATTTGGGATGATACAGCACTGATAAAAGCATATGATAAAGCTGTGGCTTCATTT AAGCATGCTCTAAAGAATGGTGACATTTGTGAAACTTCGGGTAAACCAAAAACCACACCT AAAAGAAAACCTGCTAAGAAGAATAAAAGCCAAAAGAAGAATACTGCAGCTTCCTTACAA CAGTGGAAAGTTGGGGACAAATGTTCTGCCATTTGGTCAGAAGACGGTTGCATTTACCCA GCTACCATTGCTTCAATTGATTTTAAGAGAGAAACCTGTGTTGTGGTTTACACTGGATAT GGAAATAGAGAGGAGCAAAATCTGTCCGATCTACTTTCCCCAATCTGTGAAGTAGCTAAT AATATAGAACAGAATGCTCAAGAGAATGAAAATGAAAGCCAAGTTTCAACAGATGAAAGT GAGAACTCCAGGTCTCCTGGAAATAAATCAGATAACATCAAGCCCAAATCTGCTCCATGG AACTCTTTTCTCCCTCCACCACCCCCCATGCCAGGGCCAAGACTGGGACCAGGAAAGCCA GGTCTAAAATTCAATGGCCCACCACCGCCACCGCCACCACCACCACCCCACTTACTATCA TGCTGGCTGCCTCCATTTCCTTCTGGACCACCAATAATTCCCCCACCACCTCCCATATGT CCAGATTCTCTTGATGATGCTGATGCTTTGGGAAGTATGTTAATTTCATGGTACATGAGT GGCTATCATACTGGCTATTATATGGGTTTTAGACAAAATCAAAAAGAAGGAAGGTGCTCA CATTCCTTAAATTAAGGAGAAATGCTGGCATAGAGCAGCACTAAATGACACCACTAAAGA AACGATCAGACAGATCTGGAATGTGAAGCGTTATAGAAGATAACTGGCCTCATTTCTTCA AAATATCAAGTGTTGGGAAAGAAAAAAGGAAGTGGAATGGGTAACTCTTCTTGATTAAAA GTTATGTAATAACCAAATGCAATGTGAAATATTTTACTGGACTCTATTTTGAAAAACCAT CTGTAAAAGACTGAGGTGGGGGTGGGAGGCCAGCACGGTGGTGAGGCAGTTGAGAAAATT TGAATGTGGATTAGATTTTGAATGATATTGGATAATTATTGGTAATTTTATGAGCTGTGA GAAGGGTGTTGTAGTTTATAAAAGACTGTCTTAATTTGCATACTTAAGCATTTAGGAATG AAGTGTTAGAGTGTCTTAAAATGTTTCAAATGGTTTAACAAAATGTATGTGAGGCGTATG TGGCAAAATGTTACAGAATCTAACTGGTGGACATGGCTGTTCATTGTACTGTTTTTTTCT ATCTTCTATATGTTTAAAAGTATATAATAAAAATATTTAATTTTTTTTTAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_000344 unedited
GTTTTCATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGGTCCGG AATTCCCGGGATATCGTCGACCCACGCGTCCGGGCCCCACGCTGCGCACCCGCGGGTTTG CTATGGCGATGAGCAGCGGCGGCAGTGGTGGCGGCGTCCCGGAGCAGGAGGATTCCGTGC TGTTCCGGCGCGGCACAGGCCAGAGCGATGATTCTGACATTTGGGATGATACAGCACTGA TAAAAGCATATGATAAAGCTGTGGCTTCATTTAAGCATGCTCTAAAGAATGGTGACATTT GTGAAACTTCGGGTAAACCAAAAACCACACCTAAAAGAAAACCTGCTAAGAAGAATAAAA GCCAAAAGAAGAATACTGCAGCTTCCTTACAACAGTGGAAAGTTGGGGACAAATGTTCTG CCATTTGGTCAGAAGACGGTTGCATTTACCCAGCTACCATTGCTTCAATTGATTTTAAGA GAGAAACCTGTGTTGTGGTTTACACTGGATATGGAAATAGAGAGGAGCAAAATCTGTCCG ATCTACTTTCCCCAATCTGTGAAGTAGCTAATAATATAGAACAGAATGCTCAAGAGAATG AAAATGAAAGCCAAGTTTCAACAGATGGAAAGTGAGAAACTCCAGGTCCTCCTGGAAAAT AAATCCAGATTAACATCAAGGCCCAAAATCTTGCTCCATGGGAACTCTTTTTTTTCCCGC CACCACCCCCCATGCCAAGGGCCAAGACTGGGGACCCAGGAAAGCCAGGTTTAATATTTA ATGGGCCCACCACCGGTCTCCGTCACCCACCACCCCCCCCATTTACCATTAAAGCCTGGC TGGCCTCCCATTTCCTTTGGGGACCCCCCACATAATTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_000344 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000344.2, NP_000335.1 |
| RefSeq Size | 1621 bp |
| RefSeq ORF | 885 bp |
| Locus ID | 6606 |
| UniProt ID | Q16637 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. However, mutations in this gene, the telomeric copy, are associated with spinal muscular atrophy; mutations in the centromeric copy do not lead to disease. The centromeric copy may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7, which is thought to be an exon splice enhancer. Note that the nine exons of both the telomeric and centromeric copies are designated historically as exon 1, 2a, 2b, and 3-8. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (d) represents the longest transcript and encodes the longest isoform (d). This variant is thought to be the predominant transcript produced by this copy of the gene. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene
,Rashnonejad, A;Chermahini, GA;Li, S;Ozkinay, F;Gao, G;,
Mol. Biotechnol.
,PubMed ID 26607476
[SMN1]
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|
Statins Block Calcific Nodule Formation of Valvular Interstitial Cells by Inhibiting {alpha}-Smooth Muscle Actin Expression
,Julie A. Benton, Hanna B. Kern, Leslie A. Leinwand, Peter D. Mariner, and Kristi S. Anseth,
Arterioscler. Thromb. Vasc. Biol., Aug 2009; 10.1161/ATVBAHA.109.195271
[SMN1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221367 | SMN1 (Myc-DDK-tagged)-Human survival of motor neuron 1, telomeric (SMN1), transcript variant d |
CNY 2,400.00 |
|
| RC221367L1 | Lenti ORF clone of Human survival of motor neuron 1, telomeric (SMN1), transcript variant d, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221367L2 | Lenti ORF clone of Human survival of motor neuron 1, telomeric (SMN1), transcript variant d, mGFP tagged |
CNY 5,890.00 |
|
| RC221367L3 | Lenti ORF clone of Human survival of motor neuron 1, telomeric (SMN1), transcript variant d, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221367L4 | Lenti ORF clone of Human survival of motor neuron 1, telomeric (SMN1), transcript variant d, mGFP tagged |
CNY 4,800.00 |
|
| RG221367 | SMN1 (tGFP-tagged) - Human survival of motor neuron 1, telomeric (SMN1), transcript variant d |
CNY 4,000.00 |
