Collagen IV (COL4A3) (NM_000091) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATS2; ATS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC300008 representing NM_000091. Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGAGCGCCCGGACCGCCCCCAGGCCGCAGGTGCTCCTGCTGCCGCTCCTGCTGGTGCTCCTGGCGGCG GCGCCCGCAGCCAGCAAGGGTTGTGTCTGTAAAGACAAAGGCCAGTGCTTCTGTGACGGGGCCAAAGGG GAGAAGGGGGAGAAGGGCTTTCCTGGACCCCCCGGTTCTCCTGGCCAGAAAGGATTCACAGGTCCTGAA GGCTTGCCTGGACCGCAGGGACCCAAGGGCTTTCCAGGACTTCCAGGACTCACGGGTTCCAAAGGTGTA AGGGGAATAAGTGGATTGCCAGGATTTTCTGGTTCTCCTGGACTTCCAGGCACCCCAGGCAATACCGGG CCTTACGGACTTGTCGGTGTACCAGGATGCAGTGGTTCTAAGGGTGAGCAGGGGTTTCCAGGACTCCCA GGGACACTGGGCTACCCAGGGATCCCGGGTGCTGCTGGTTTGAAAGGACAAAAGGGTGCTCCTGCTAAA GAAGAAGATATAGAACTTGATGCAAAAGGCGACCCCGGGTTGCCAGGGGCTCCAGGACCCCAGGGTTTG CCAGGCCCTCCAGGTTTTCCTGGGCCTGTTGGCCCACCTGGTCCTCCGGGATTCTTTGGCTTTCCAGGA GCCATGGGACCTAGAGGACCTAAGGGTCACATGGGTGAAAGAGTGATAGGACATAAAGGAGAGCGGGGT GTGAAAGGGTTAACAGGACCCCCGGGACCACCAGGAACAGTTATTGTGACCCTAACTGGCCCAGATAAC AGAACGGACCTCAAGGGGGAAAAGGGAGACAAGGGAGCAATGGGCGAGCCTGGACCTCCTGGACCCTCA GGACTGCCTGGAGAATCATATGGATCTGAAAAGGGTGCTCCTGGAGACCCTGGCCTGCAGGGAAAACCC GGAAAAGATGGTGTTCCTGGCTTCCCTGGAAGTGAGGGAGTCAAGGGCAACAGGGGTTTCCCTGGGTTA ATGGGTGAAGATGGCATTAAGGGACAGAAAGGGGACATTGGCCCTCCAGGATTTCGTGGTCCAACAGAA TATTATGACACATACCAGGAAAAGGGAGATGAAGGCACTCCAGGCCCACCAGGGCCCAGAGGAGCTCGT GGCCCACAAGGTCCCAGTGGTCCCCCCGGAGTTCCTGGAAGTCCTGGATCATCAAGGCCTGGCCTCAGA GGAGCCCCTGGATGGCCAGGCCTGAAAGGAAGTAAAGGGGAACGAGGCCGCCCAGGAAAGGATGCCATG GGGACTCCTGGGTCCCCAGGTTGTGCTGGTTCACCAGGTCTTCCAGGATCACCGGGACCTCCAGGACCG CCAGGTGACATCGTTTTTCGCAAGGGTCCACCTGGAGATCACGGACTGCCAGGCTATCTAGGGTCTCCA GGAATCCCAGGAGTTGATGGGCCCAAAGGAGAACCAGGCCTCCTGTGTACACAGTGCCCTTATATCCCA GGGCCTCCCGGTCTCCCAGGATTGCCAGGGTTACATGGTGTAAAAGGAATCCCAGGAAGACAAGGCGCA GCTGGCTTGAAAGGAAGCCCAGGGTCCCCAGGAAATACAGGTCTTCCAGGATTTCCAGGTTTCCCAGGT GCCCAGGGTGACCCAGGACTTAAAGGAGAAAAAGGTGAAACACTTCAGCCTGAGGGGCAAGTGGGTGTC CCAGGTGACCCGGGGCTCAGAGGCCAACCTGGGAGAAAGGGCTTGGATGGAATTCCTGGAACTCCGGGA GTGAAAGGATTACCAGGACCTAAAGGCGAACTGGCTCTGAGTGGTGAGAAAGGGGACCAAGGTCCTCCA GGGGATCCTGGCTCCCCTGGGTCCCCAGGACCTGCAGGACCAGCTGGACCACCTGGCTACGGACCCCAA GGAGAACCTGGTCTCCAGGGCACGCAAGGAGTTCCTGGAGCCCCCGGACCACCCGGAGAAGCCGGCCCT AGGGGAGAGCTCAGTGTTTCAACACCAGTTCCAGGCCCACCAGGACCTCCAGGGCCCCCTGGCCATCCT GGCCCCCAAGGTCCACCTGGTATCCCTGGATCCCTGGGGAAATGTGGAGATCCTGGTCTTCCAGGGCCT GATGGTGAACCAGGAATTCCAGGAATTGGATTTCCTGGGCCTCCTGGACCTAAGGGAGACCAAGGTTTT CCAGGTACAAAAGGATCACTGGGTTGTCCTGGAAAAATGGGAGAGCCTGGGTTACCTGGAAAGCCAGGC CTCCCAGGAGCCAAGGGAGAACCAGCAGTAGCCATGCCTGGAGGACCAGGAACACCAGGTTTTCCAGGA GAAAGAGGCAATTCTGGGGAACATGGAGAAATTGGACTCCCTGGACTTCCAGGTCTCCCTGGAACTCCA GGAAATGAAGGGCTTGATGGACCACGAGGAGATCCAGGGCAGCCTGGACCACCTGGAGAACAAGGACCC CCAGGAAGGTGCATAGAGGGTCCCAGGGGAGCCCAAGGACTTCCAGGCTTAAATGGATTGAAAGGGCAA CAAGGCAGAAGAGGTAAAACGGGGCCAAAGGGAGACCCAGGAATTCCAGGCTTGGATAGATCAGGATTT CCTGGAGAAACTGGATCACCAGGAATTCCAGGTCATCAAGGTGAAATGGGACCACTGGGTCAAAGAGGA TATCCAGGAAATCCGGGAATTTTAGGGCCACCAGGTGAAGATGGAGTGATTGGGATGATGGGCTTTCCT GGAGCCATTGGCCCTCCAGGGCCCCCTGGGAACCCAGGCACACCAGGGCAGAGGGGGAGCCCTGGAATT CCAGGAGTAAAGGGCCAGAGAGGAACCCCAGGAGCCAAGGGGGAACAAGGAGATAAAGGAAATCCCGGG CCTTCAGAGATATCCCACGTAATAGGGGACAAAGGAGAACCAGGTCTCAAAGGATTCGCAGGAAATCCA GGTGAGAAAGGAAACAGAGGCGTTCCAGGGATGCCAGGTTTAAAGGGCCTCAAAGGACTACCCGGACCA GCAGGACCACCAGGCCCCAGAGGAGATTTGGGCAGCACTGGGAATCCTGGAGAACCAGGACTGCGTGGT ATACCAGGAAGCATGGGGAACATGGGCATGCCAGGTTCTAAAGGAAAAAGGGGAACTTTGGGATTCCCA GGTCGAGCAGGAAGACCAGGCCTCCCAGGTATTCATGGTCTCCAGGGAGATAAGGGAGAGCCAGGTTAT TCAGAAGGTACAAGGCCAGGACCACCGGGACCAACGGGGGATCCAGGACTGCCGGGTGATATGGGAAAG AAAGGAGAAATGGGGCAACCTGGCCCACCTGGACATTTGGGGCCTGCTGGACCTGAGGGAGCCCCTGGA AGTCCTGGAAGTCCTGGCCTCCCAGGAAAGCCAGGTCCTCATGGTGATTTGGGTTTTAAAGGAATCAAA GGCCTCCTGGGCCCTCCAGGAATCAGAGGCCCTCCAGGTCTTCCAGGATTTCCAGGATCTCCTGGACCA ATGGGTATAAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCAGCCGGAGAAAAGGGAGAAACGGGT TTATTGAGGGCCCCTCCAGGCCCAAGAGGGAACCCTGGTGCTCAAGGAGCCAAAGGAGACAGGGGAGCC CCAGGTTTTCCTGGCCTCCCGGGCAGAAAAGGGGCCATGGGAGATGCTGGACCTCGAGGACCCACAGGC ATAGAAGGATTCCCAGGGCCACCAGGTCTGCCCGGTGCAATTATCCCTGGCCAGACAGGAAATCGTGGT CCACCAGGCTCAAGAGGAAGCCCAGGTGCGCCTGGTCCCCCTGGACCTCCAGGGAGTCATGTAATAGGC ATAAAAGGAGACAAAGGGTCTATGGGCCACCCTGGCCCAAAAGGTCCACCTGGAACTGCAGGAGACATG GGACCACCAGGTCGTCTGGGAGCACCAGGTACTCCAGGTCTTCCAGGACCCAGAGGTGATCCTGGATTC CAGGGGTTTCCAGGCGTGAAAGGAGAAAAGGGTAATCCTGGATTTCTAGGATCCATTGGACCTCCAGGA CCAATTGGGCCAAAAGGACCACCTGGTGTACGTGGAGACCCTGGCACACTTAAGATTATCTCCCTTCCA GGAAGCCCAGGGCCACCTGGCACACCTGGAGAACCAGGGATGCAGGGAGAACCTGGGCCACCAGGGCCA CCTGGAAACCTAGGACCCTGTGGGCCAAGAGGTAAGCCAGGCAAGGATGGAAAACCAGGAACTCCTGGA CCAGCTGGAGAAAAAGGCAACAAAGGTTCTAAAGGAGAGCCAGGACCAGCTGGATCAGATGGATTGCCA GGTTTGAAAGGAAAACGTGGAGACAGTGGATCACCTGCAACCTGGACAACGAGAGGCTTTGTCTTCACC CGACACAGTCAAACCACAGCAATTCCTTCATGTCCAGAGGGGACAGTGCCACTCTACAGTGGGTTTTCT TTTCTTTTTGTACAAGGAAATCAACGAGCCCACGGACAAGACCTTGGAACTCTTGGCAGCTGCCTGCAG CGATTTACCACAATGCCATTCTTATTCTGCAATGTCAATGATGTATGTAATTTTGCATCTCGAAATGAT TATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGAACATGGCTCCCATTACTGGCAGAGCCCTT GAGCCTTATATAAGCAGATGCACTGTTTGTGAAGGTCCTGCGATCGCCATAGCCGTTCACAGCCAAACC ACTGACATTCCTCCATGTCCTCACGGCTGGATTTCTCTCTGGAAAGGATTTTCATTCATCATGTTCACA AGTGCAGGTTCTGAGGGCACCGGGCAAGCACTGGCCTCCCCTGGCTCCTGCCTGGAAGAATTCCGAGCC AGCCCATTTCTAGAATGTCATGGAAGAGGAACGTGCAACTACTATTCAAATTCCTACAGTTTCTGGCTG GCTTCATTAAACCCAGAAAGAATGTTCAGAAAGCCTATTCCATCAACTGTGAAAGCTGGGGAATTAGAA AAAATAATAAGTCGCTGTCAGGTGTGCATGAAGAAAAGACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	AscI-MluI
ACCN	NM_000091
Insert Size	5013 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000091.4
RefSeq Size	8114 bp
RefSeq ORF	5013 bp
Locus ID	1285
UniProt ID	Q01955
Protein Families	Druggable Genome
MW	161.8 kDa
Gene Summary	Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.