United States
Germany
Japan
United Kingdom
China
PDX1 (NM_000209) Human Untagged Clone
CAT＃: SC300025
PDX1 (untagged)-Human pancreatic and duodenal homeobox 1 (PDX1)
CNY 2,400.00
CNY 6,270.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000209 edited
ATGAACGGCGAGGAGCAGTACTACGCGGCCACGCAGCTTTACAAGGACCCATGCGCGTTC CAGCGAGGCCCGGCGCCGGAGTTCAGCGCCAGCCCCCCTGCGTGCCTGTACATGGGCCGC CAGCCCCCGCCGCCGCCGCCGCACCCGTTCCCTGGCGCCCTGGGCGCGCTGGAGCAGGGC AGCCCCCCGGACATCTCCCCGTACGAGGTGCCCCCCCTCGCCGACGACCCCGCGGTGGCG CACCTTCACCACCACCTCCCGGCTCAGCTCGCGCTCCCCCACCCGCCCGCCGGGCCCTTC CCGGAGGGAGCCGAGCCGGGCGTCCTGGAGGAGCCCAACCGCGTCCAGCTGCCTTTCCCA TGGATGAAGTCTACCAAAGCTCACGCGTGGAAAGGCCAGTGGGCAGGCGGCGCCTACGCT GCGGAGCCGGAGGAGAACAAGCGGACGCGCACGGCCTACACGCGCGCACAGCTGCTAGAG CTGGAGAAGGAGTTCCTATTCAACAAGTACATCTCACGGCCGCGCCGGGTGGAGCTGGCT GTCATGTTGAACTTGACCGAGAGACACATCAAGATCTGGTTCCAAAACCGCCGCATGAAG TGGAAAAAGGAGGAGGACAAGAAGCGCGGCGGCGGGACAGCTGTCGGGGGTGGCGGGGTC GCGGAGCCTGAGCAGGACTGCGCCGTGACCTCCGGCGAGGAGCTTCTGGCGCTGCCGCCG CCGCCGCCCCCCGGAGGTGCTGTGCCGCCCGCTGCCCCCGTTGCCGCCCGAGAGGGCCGC CTGCCGCCTGGCCTTAGCGCGTCGCCACAGCCCTCCAGCGTCGCGCCTCGGCGGCCGCAG GAACCACGATGA >OriGene 5' read for NM_000209 unedited
GGACGTTCACATTTGTATACGACTCATATAGGCGGCCGCGNATTCATGAACGGCGAGGAG CAGTACTACGCGGCCACGCAGCTTTACAAGGACCCATGCGCGTTCCAGCGAGGCCCGGCG CCGGAGTTCAGCGCCAGCCCCCCTGCGTGCCTGTACATGGGCCGCCAGCCCCCGCCGCCG CCGCCGCACCCGTTCCCTGGCGCCCTGGGCGCGCTGGAGCAGGGCAGCCCCCCTGACATC TCCCCGTACGAGGTGCCCCCCCTCGCCGACGACCCCGCGGTGGCGCACCTTCACCACCAC CTCCCGGCTCAGCTCGCGCTCCCCCACCCGCCCGCCGGGCCCTTCCCGGAGGGAGCCGAA CCGGGCGTCCTGGAGGAGCCCAACCGCGTCCAGCTGCCTTTCCCATGGATGAAGTCTACC AAAGCTCACGCGTGGAAAGGCCAGTGGGCAGGCGGCGCCTACGCTGCGGAGCCGGAGGAG AACAAGCGGACGCGCACGGCCTACACGCGCGCACAGCTGCTAGAGCTGGAGAAGGAGTTC CTATTCAACAAGTACATCTCACGGCCGCGCCGGGTGGAGCTGGCTGTCATGTTGAACTTG ACCGAGAGACACATCAAGATCTGGTTCCAAAACCGCCGCATGAAGTGGAAAAAGGAGGAG GACAAGAAGCGCGGCGGCGGGACAGCTGTCGGGGGTGGGCGGNNGTCGCGGAGCCTGAGC AGGACTGCGCCGTGACCTCCGGCGAGGAGCTTTGGGCGCTGCCGCCGCCGCGCCCCCGGG GAGGGGGCTGTGCCGCCCGCTGCCCCCGTTGCCGCCCGAGAGGGCCGCCTGCCGCCTGGC CTTAGCGCGTCGGCACAGCCCTTCAGCGTGGCGCCTCGGGCGGCCGCAGGAACCACGG |
| Restriction Sites | Please inquire |
| ACCN | NM_000209 |
| Insert Size | 900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There are 2 nucleotide differences between the OriGene clone and the NCBI reference ORF, but these are silent and do not change the amino acid sequence of the deduced protein. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000209.1, NP_000200.1 |
| RefSeq Size | 1525 bp |
| RefSeq ORF | 852 bp |
| Locus ID | 3651 |
| UniProt ID | P52945 |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| Protein Pathways | Maturity onset diabetes of the young, Type II diabetes mellitus |
| Gene Summary | The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Aug 2017] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pancreatic and duodenal homeobox gene 1 (Pdx1) down-regulates hepatic transcription factor 1 alpha (HNF1a) expression during reprogramming of human hepatic cells into insulin-producing cells
,Donelan, W;Li, S;Wang, H;Lu, S;Xie, C;Tang, D;Chang, LJ;Yang, LJ;,
Am J Transl Res
,PubMed ID 26279745
[PDX1]
|
|
Pancreatic and duodenal homeobox gene 1 (Pdx1)down-regulates hepatic transcription factor1 alpha (HNF1a) expression duringreprogramming of human hepaticcells into insulin-producing cells
,Donelan, W;Li, S;Wang, H;Lu, S;Xie, C;Tang, D;,
Am J Transl Res
[PDX1]
|
|
Distinct Regulation of Hepatic Nuclear Factor 1{alpha} by NKX6.1 in Pancreatic Beta Cells
,William Donelan, Vijay Koya, Shi-Wu Li, and Li-Jun Yang,
J. Biol. Chem., Apr 2010; 285: 12181 - 12189
[PDX1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222354 | PDX1 (Myc-DDK-tagged)-Human pancreatic and duodenal homeobox 1 (PDX1) |
CNY 3,600.00 |
|
| RC222354L1 | Lenti ORF clone of Human pancreatic and duodenal homeobox 1 (PDX1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC222354L2 | Lenti ORF clone of Human pancreatic and duodenal homeobox 1 (PDX1), mGFP tagged |
CNY 6,000.00 |
|
| RC222354L3 | Lenti ORF clone of Human pancreatic and duodenal homeobox 1 (PDX1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222354L4 | Lenti ORF clone of Human pancreatic and duodenal homeobox 1 (PDX1), mGFP tagged |
CNY 6,000.00 |
|
| RG222354 | PDX1 (tGFP-tagged) - Human pancreatic and duodenal homeobox 1 (PDX1) |
CNY 5,200.00 |
