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ATP4A (NM_000704) Human Untagged Clone
CAT＃: SC300122
ATP4A (untagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
CNY 7,232.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATP6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300122 representing NM_000704.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAAGGCCGAGAACTATGAGCTCTACTCGGTGGAGCTGGGTCCTGGCCCTGGCGGGGACATGGCT GCCAAGATGAGCAAGAAGAAGAAGGCGGGTGGCGGGGGTGGCAAGAGGAAGGAGAAGCTGGAGAACATG AAGAAGGAGATGGAGATTAACGACCACCAGCTGTCAGTGGCGGAGCTGGAACAGAAATACCAGACCAGT GCCACCAAGGGCCTCTCTGCGAGCCTGGCTGCTGAGCTGCTGCTGCGGGATGGGCCCAACGCACTGCGG CCACCACGGGGCACCCCAGAGTACGTCAAGTTTGCGAGGCAGCTGGCCGGGGGCCTGCAGTGCCTCATG TGGGTTGCCGCCGCCATCTGCCTCATCGCCTTTGCCATCCAGGCTAGTGAGGGGGACCTCACCACCGAC GACAATCTGTACCTGGCAATCGCTCTCATTGCTGTGGTTGTCGTCACCGGCTGCTTTGGCTACTACCAG GAATTCAAGAGCACCAACATCATCGCCAGCTTTAAGAACCTTGTGCCACAGCAAGCCACTGTCATCCGC GATGGAGACAAATTCCAGATCAACGCTGACCAACTGGTGGTGGGCGACCTGGTGGAGATGAAAGGTGGG GACAGAGTGCCCGCCGACATCCGCATCCTGGCGGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTGACA GGGGAGTCTGAGCCACAGACCCGCTCACCCGAGTGCACGCACGAGAGCCCTCTGGAGACCCGCAACATC GCCTTCTTCTCCACCATGTGCCTTGAGGGCACCGTGCAGGGCCTGGTGGTGAACACGGGCGACCGCACC ATCATTGGGCGCATCGCATCGCTGGCGTCGGGGGTGGAAAACGAGAAGACACCCATCGCTATCGAGATC GAGCATTTTGTGGACATCATCGCGGGCCTGGCCATTCTCTTCGGTGCCACATTTTTTATTGTGGCCATG TGCATTGGCTACACCTTCCTGCGGGCCATGGTCTTCTTCATGGCCATCGTGGTGGCCTATGTGCCTGAG GGGCTGCTGGCCACTGTCACAGTCTGCCTGTCCCTGACAGCCAAGCGCCTGGCCAGTAAGAACTGCGTG GTCAAGAACCTGGAGGCGGTGGAGACATTGGGCTCCACTTCGGTGATCTGCTCGGACAAGACAGGGACT CTCACTCAGAACCGCATGACTGTGTCCCATCTGTGGTTTGACAACCACATCCACACAGCTGACACCACG GAAGACCAGTCAGGGCAGACGTTTGACCAGTCCTCGGAGACGTGGCGGGCGCTGTGCCGGGTGCTCACC CTGTGCAACCGCGCCGCCTTCAAGTCCGGCCAGGATGCAGTGCCTGTGCCCAAGCGCATCGTGATTGGA GACGCATCGGAGACGGCGCTGCTCAAGTTCTCGGAGCTGACGCTGGGCAACGCCATGGGCTACCGGGAC CGCTTCCCAAAAGTCTGCGAGATACCCTTCAACTCCACCAACAAGTTCCAGCTGTCCATCCATACGCTG GAGGACCCGCGGGACCCGCGACACTTGCTGGTGATGAAGGGCGCCCCCGAGCGCGTGCTGGAGCGCTGC AGCTCCATCCTTATCAAGGGCCAGGAGCTGCCGCTGGACGAGCAGTGGCGCGAGGCCTTCCAGACCGCC TACCTCAGCCTGGGAGGCCTGGGCGAACGCGTGCTCGGCTTCTGCCAGCTCTACCTGAATGAGAAGGAC TACCCGCCTGGCTATGCCTTCGACGTAGAGGCCATGAACTTTCCATCTAGCGGCCTCTGCTTTGCGGGA CTTGTATCCATGATTGACCCACCCCGGGCCACCGTCCCTGATGCTGTGCTCAAGTGTCGCACCGCAGGC ATCCGGGTGATCATGGTAACGGGTGACCACCCCATCACCGCCAAGGCCATTGCAGCCAGTGTGGGCATC ATCTCGGAAGGCAGCGAGACAGTGGAGGACATCGCTGCCCGCCTCCGTGTGCCCGTAGACCAGGTTAAT CGCAAGGATGCCCGTGCCTGTGTGATCAATGGCATGCAGCTGAAGGACATGGACCCATCGGAACTGGTC GAGGCCCTGCGCACCCACCCCGAGATGGTGTTTGCGCGCACCAGCCCCCAGCAGAAGCTGGTGATCGTG GAGAGCTGCCAGCGGCTGGGTGCGATTGTGGCCGTCACGGGGGATGGTGTGAATGACTCCCCAGCTCTG AAGAAGGCAGACATCGGAGTAGCCATGGGCATCGCTGGCTCAGATGCTGCCAAAAATGCAGCTGACATG ATCCTGCTGGATGACAACTTTGCCTCCATTGTGACAGGCGTGGAGCAGGGTCGACTGATCTTCGACAAC CTGAAGAAGTCTATTGCCTACACATTGACCAAGAACATCCCAGAGCTGACACCCTACCTCATCTACATC ACCGTCAGCGTGCCCCTGCCCCTCGGGTGCATCACCATCCTCTTCATCGAACTCTGCACTGACATTTTC CCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGTGACATCATGCACCTGCGTCCACGCAACCCAAAG CGTGACAGATTGGTCAACGAGCCCCTGGCTGCCTACTCCTACTTCCAGATTGGTGCCATTCAGTCCTTT GCTGGCTTCACTGACTACTTCACGGCAATGGCCCAGGAGGGCTGGTTCCCACTGCTGTGCGTGGGGCTG CGGGCGCAGTGGGAGGACCACCACCTACAAGATCTGCAGGACAGCTACGGCCAGGAGTGGACATTCGGG CAGCGCCTGTACCAGCAGTACACCTGCTACACCGTGTTCTTCATCAGCATTGAGGTGTGCCAGATCGCC GATGTCCTCATCCGCAAGACGCGCCGTCTCTCTGCCTTCCAGCAAGGCTTCTTCAGGAATAAGATCCTG GTGATCGCCATCGTGTTCCAGGTCTGCATCGGCTGCTTCCTGTGCTACTGCCCCGGCATGCCCAACATC TTCAACTTCATGCCCATTCGGTTCCAGTGGTGGCTGGTCCCCCTGCCCTACGGCATCCTCATCTTCGTC TATGATGAGATCCGGAAGCTTGGAGTTCGCTGTTGCCCAGGGAGCTGGTGGGACCAGGAACTCTACTA TAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_000704 |
| Insert Size | 3117 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000704.2 |
| RefSeq Size | 3582 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 495 |
| UniProt ID | P20648 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Oxidative phosphorylation |
| MW | 114.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211772 | ATP4A (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) |
CNY 7,232.00 |
|
| RC211772L1 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged |
CNY 9,632.00 |
|
| RC211772L2 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged |
CNY 10,260.00 |
|
| RC211772L3 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC211772L4 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged |
CNY 10,260.00 |
|
| RG211772 | ATP4A (tGFP-tagged) - Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) |
CNY 8,832.00 |
