DSCAM (NM_001389) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CHD2; CHD2-42; CHD2-52
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303008 representing NM_001389. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGACCTACACTCCAGC CTCTACTTTGTCAATGCATCTCTGCAAGAGGTAGTGTTTGCCAGCACCACGGGGACTCTGGTGCCCTGC CCCGCAGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCCACGGGCGAGGAGATCTACGATGTC CCCGGGATCCGCCACGTCCACCCCAACGGCACTCTCCAAATTTTCCCCTTCCCTCCTTCAAGCTTCAGT ACCTTAATCCATGATAATACTTATTATTGCACAGCTGAAAATCCTTCAGGGAAAATTAGAAGTCAGGAT GTCCACATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGC AATGTTGCGGTCTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACATCACTGTCGTCTCATGGGAG AAAGACACTGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAA GATGTACAGAATGAAGATGGATTGTATAACTACCGCTGCATCACGCGGCATCGATACACCGGAGAGACG AGGCAGAGCAACAGCGCCAGACTTTTTGTATCAGACCCAGCGAACTCAGCCCCATCCATACTGGATGGG TTTGACCATCGCAAAGCCATGGCTGGGCAGCGTGTGGAGCTGCCTTGCAAAGCGCTCGGGCACCCTGAG CCAGATTACCGCTGGCTGAAGGACAACATGCCCCTGGAACTTTCAGGGAGGTTCCAGAAGACCGTGACG GGGCTGCTCATTGAGAACATTCGCCCCTCGGACTCAGGCAGCTATGTTTGTGAAGTGTCCAACAGATAC GGAACTGCTAAGGTGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGGAAG GTTAAAAGCAGCGTGGGTAGCCAAGTTTCCTTGTCCTGCAGCGTGACAGGAACTGAGGACCAGGAACTC TCCTGGTACCGCAATGGTGAAATCCTCAACCCTGGAAAAAATGTGAGGATCACAGGGATCAACCACGAA AACCTTATAATGGATCACATGGTCAAAAGTGACGGGGGCGCATACCAGTGCTTTGTGCGCAAGGACAAG CTGTCCGCTCAAGACTATGTGCAGGTGGTCCTTGAAGATGGAACTCCCAAAATTATTTCTGCCTTTAGT GAAAAGGTGGTGAGTCCAGCAGAGCCGGTTTCCCTTATGTGCAACGTGAAGGGAACACCTTTGCCCACG ATCACGTGGACCCTGGACGATGACCCGATTCTCAAGGGTGGCAGTCACCGCATCAGCCAGATGATCACG TCGGAGGGGAACGTGGTCAGCTACCTGAACATCTCCAGCTCCCAGGTCCGGGACGGGGGAGTCTACCGC TGCACTGCCAACAACTCGGCGGGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCTGCAAGC ATTCGACCAATGAAAAACATCACAGCAATAGCAGGACGGGACACATACATTCACTGTCGTGTGATTGGC TATCCGTATTACTCCATTAAATGGTACAAGAACTCTAACCTGCTTCCTTTCAACCACCGCCAAGTGGCA TTTGAGAACAATGGAACTCTTAAACTTTCAGATGTGCAAAAGGAAGTGGACGAGGGGGAGTACACGTGC AACGTGTTGGTTCAACCACAACTCTCCACCAGCCAGAGCGTCCACGTGACCGTGAAAGTTCCGCCTTTC ATACAACCCTTTGAGTTTCCAAGATTCTCCATTGGGCAGCGGGTCTTCATCCCCTGTGTTGTGGTCTCA GGGGACTTACCCATCACGATCACCTGGCAGAAGGATGGCCGGCCAATCCCTGGGAGCCTTGGGGTGACC ATTGACAATATTGACTTCACGAGCTCCTTGAGGATTTCCAATCTCTCGCTCATGCACAATGGGAATTAC ACCTGCATAGCCCGGAATGAGGCCGCCGCTGTGGAGCACCAAAGCCAGTTGATTGTCAGAGTTCCTCCC AAGTTTGTGGTTCAGCCACGGGACCAGGACGGGATTTATGGCAAAGCAGTCATCCTCAATTGTTCTGCT GAGGGTTACCCTGTACCTACCATCGTGTGGAAATTCTCTAAAGGTGCTGGGGTTCCCCAGTTCCAGCCA ATTGCCCTAAATGGCCGAATCCAAGTTCTCAGCAATGGGTCGTTGCTGATCAAGCATGTCGTGGAGGAA GACAGTGGCTACTACCTCTGCAAGGTCAGCAACGATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTC ACGGTTAAAATTCCTGCGATGATAACATCCTATCCAAATACTACCCTGGCCACGCAGGGGCAGAAAAAG GAGATGAGCTGCACGGCGCATGGTGAGAAGCCCATTATAGTCCGCTGGGAGAAGGAGGACCGAATCATT AACCCTGAGATGGCCCGTTATCTTGTGTCCACCAAGGAGGTGGGAGAAGAGGTGATTTCTACTCTGCAG ATTTTGCCAACTGTGAGAGAAGATTCTGGTTTCTTTTCCTGCCATGCTATTAATTCTTATGGGGAGGAC CGTGGAATAATTCAGCTCACAGTGCAAGAGCCCCCAGACCCTCCCGAAATTGAGATCAAAGATGTCAAA GCACGCACAATTACGCTCAGGTGGACCATGGGGTTTGATGGAAACAGTCCCATCACAGGCTACGATATT GAATGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAGAGAACCAAAGATGTTTCCCCTCAGCTGAAC TCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAGCATCCGCATGTACGCCAAGAACCGGATT GGCAAGAGCGAGCCCAGCAACGAGCTCACCATCACGGCGGACGAGGCAGCTCCTGATGGTCCACCTCAG GAAGTTCACCTGGAGCCTATATCATCTCAGAGCATCAGGGTCACATGGAAGGCTCCCAAGAAACATTTG CAAAATGGGATTATCCGTGGCTACCAAATAGGTTACCGAGAGTACAGCACTGGGGGTAACTTCCAATTC AACATTATCAGTGTCGACACCAGCGGGGACAGTGAGGTTTACACCCTGGACAACCTGAATAAGTTCACT CAGTACGGCCTGGTGGTGCAGGCCTGTAACCGGGCCGGCACGGGGCCTTCTTCTCAGGAAATCATCACC ACCACTCTCGAGGATGTGCCCAGTTACCCCCCCGAAAATGTCCAAGCCATAGCAACATCACCAGAAAGC ATATCAATATCCTGGTCCACACTTTCCAAGGAAGCCTTGAATGGAATTCTCCAGGGGTTCAGAGTCATT TACTGGGCCAACCTCATGGACGGAGAGCTGGGTGAGATTAAAAACATCACCACCACACAGCCTTCACTG GAGCTGGACGGGCTGGAAAAGTACACCAACTACAGCATCCAGGTGCTGGCCTTCACCCGCGCAGGAGAC GGGGTCAGGAGTGAGCAGATCTTCACCCGGACCAAAGAGGATGTTCCAGGTCCTCCCGCGGGTGTGAAG GCAGCGGCGGCCTCAGCCTCCATGGTCTTTGTGTCCTGGCTTCCCCCTCTCAAGCTGAACGGCATCATC CGAAAGTACACTGTATTCTGCTCCCACCCCTATCCCACAGTGATCAGCGAGTTTGAGGCCTCTCCCGAC TCGTTTTCCTACAGAATTCCCAACCTGAGTAGGAATCGTCAGTACAGCGTCTGGGTGGTGGCTGTTACT TCAGCCGGAAGAGGCAACAGCAGTGAAATCATCACAGTCGAGCCACTAGCAAAAGCTCCTGCACGAATC CTGACCTTCAGTGGGACAGTGACTACTCCATGGATGAAAGACATTGTCTTGCCTTGTAAGGCTGTTGGG GACCCTTCTCCTGCAGTCAAATGGATGAAAGACAGTAACGGGACACCCAGTCTAGTAACGATTGATGGG CGGAGGAGCATCTTTAGCAACGGAAGCTTCATTATTCGCACGGTGAAAGCAGAAGACTCCGGCTATTAC AGCTGCATTGCCAATAACAACTGGGGATCTGATGAAATTATTTTAAACTTACAAGTACAAGTTCCACCA GATCAGCCTCGGCTTACAGTCTCCAAGACCACGTCTTCCTCCATCACCCTTTCTTGGCTCCCTGGAGAC AACGGGGGCAGCTCTATCAGAGGATACATACTGCAGTACTCCGAGGACAATAGTGAGCAGTGGGGGAGT TTTCCAATCAGCCCCAGCGAACGTTCCTATCGCTTGGAAAATCTCAAATGTGGGACTTGGTATAAGTTC ACACTGACAGCCCAAAATGGAGTGGGCCCAGGGCGCATAAGTGAAATCATAGAAGCAAAGACCTTAGGA AAAGAGCCCCAGTTCTCAAAGGAGCAGGAGCTGTTTGCCAGCATCAACACCACACGCGTGAGGCTGAAC CTCATTGGCTGGAATGATGGCGGCTGCCCCATCACCTCCTTCACACTAGAGTACAGGCCCTTTGGGACC ACAGTTTGGACCACAGCTCAGAGGACCTCTCTCTCCAAGTCCTACATCCTGTATGACCTGCAGGAAGCC ACCTGGTATGAGCTGCAGATGCGGGTGTGCAACAGTGCGGGCTGCGCGGAGAAGCAGGCCAACTTCGCT ACGCTGAACTACGATGGCAGTACAATTCCTCCACTCATTAAGTCAGTTGTCCAAAACGAAGAAGGGCTG ACGACCAACGAGGGGCTCAAGATGCTGGTGACCATCTCCTGTATCCTGGTGGGGGTCTTGCTGCTGTTT GTGCTCCTGCTGGTTGTGCGGAGGAGGCGGCGGGAGCAGAGGCTAAAGAGGCTGCGAGATGCAAAGAGT TTAGCTGAAATGCTCATGAGTAAGAATACCCGGACTTCAGATACGTTAAGCAAGCAACAGCAGACCCTG CGAATGCACATCGACATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACGATGGAGACCATTGAT GATCGCTCCACGGTTCTGTTGACGGATGCTGACTTTGGAGAGGCAGCTAAGCAGAAGTCCCTGACGGTC ACTCACACGGTCCATTACCAATCGGTGTCTCAGGCCACTGGGCCCTTAGTGGATGTTTCAGACGCTCGG CCGGGAACGAATCCCACCACCAGGAGGAATGCCAAGGCTGGGCCCACAGCGAGAAACCGCTATGCCAGC CAGTGGACCCTCAACCGACCCCACCCCACCATCTCAGCACACACCCTCACCACAGACTGGAGGCTGCCA ACACCCAGGGCTGCAGGATCAGTAGACAAAGAGAGCGACAGTTACAGCGTCAGCCCCTCGCAAGACACA GATCGAGCAAGAAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCCACTTACGAAGAACTGGCCAGGGCC TACGAACACGCCAAGATGGAAGAGCAACTGAGGCACGCCAAGTTCACCATCACGGAGTGCTTCATATCA GACACGTCATCGGAGCAGTTGACGGCAGGGACAAATGAGTACACGGACAGTCTGACCTCCAGCACCCCT TCCGAATCGGGAATCTGCAGGTTCACTGCATCTCCCCCCAAACCTCAGGATGGAGGAAGAGTAATGAAT ATGGCAGTTCCAAAGGCACATCGGCCAGGTGACCTCATACATTTGCCTCCATACCTTAGAATGGACTTT TTGTTAAACCGAGGTGGTCCAGGCACCAGCAGGGACCTGAGCTTAGGACAAGCATGCTTGGAACCTCAG AAAAGCCGGACCCTGAAGCGCCCCACGGTCCTGGAGCCCATCCCGATGGAAGCCGCCTCCTCCGCCTCC TCCACGAGAGAAGGACAGTCGTGGCAGCCGGGGGCCGTGGCCACATTACCTCAGCGGGAGGGAGCAGAG CTGGGACAGGCAGCTAAAATGAGCAGCTCCCAAGAATCACTGCTCGACTCCCGGGGCCATTTGAAAGGA AACAATCCTTACGCAAAATCTTACACCCTGGTATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001389
Insert Size	6039 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001389.3
RefSeq Size	7110 bp
RefSeq ORF	6039 bp
Locus ID	1826
UniProt ID	O60469
Protein Families	Transmembrane
MW	222.3 kDa
Gene Summary	This gene is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs), and is involved in human central and peripheral nervous system development. This gene is a candidate for Down syndrome and congenital heart disease (DSCHD). A gene encoding a similar Ig-CAM protein is located on chromosome 11. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (1) encodes the longer isoform (CHD2-42). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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