Plexin B1 (PLXNB1) (NM_002673) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PLEXIN-B1; PLXN5; SEP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303234 representing NM_002673. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTGCTCTGGGCCCAGCTCTTCTCCAGGCTCTCTGGGCCGGGTGGGTCCTCACCCTCCAGCCCCTT CCACCAACTGCATTCACTCCCAATGGCACGTATCTGCAGCACCTGGCAAGGGACCCCACCTCAGGCACC CTCTACCTGGGGGCTACCAACTTCCTGTTCCAGCTGAGCCCTGGGCTGCAGCTGGAGGCCACAGTGTCC ACCGGCCCTGTGCTAGACAGCAGGGACTGCCTGCCACCTGTGATGCCTGATGAGTGCCCCCAGGCCCAG CCTACCAACAACCCGAATCAGCTGCTCCTGGTGAGCCCAGGGGCCCTGGTGGTATGCGGGAGCGTGCAC CAGGGGGTCTGTGAACAGCGGCGCCTGGGGCAGCTCGAGCAGCTGCTGCTGCGGCCAGAGCGGCCTGGG GACACACAATATGTGGCTGCCAATGATCCTGCGGTCAGCACGGTGGGGCTGGTAGCCCAGGGCTTGGCA GGGGAGCCCCTCCTGTTTGTGGGGCGAGGATACACCAGCAGGGGTGTGGGGGGTGGCATTCCACCCATC ACAACCCGGGCCCTGTGGCCGCCCGACCCCCAAGCTGCCTTCTCCTATGAGGAGACAGCCAAGCTGGCA GTGGGCCGCCTCTCCGAGTACAGCCACCACTTCGTGAGTGCCTTTGCACGTGGGGCCAGCGCCTACTTC CTGTTCCTGCGGCGGGACCTGCAGGCTCAGTCTAGAGCTTTTCGTGCCTATGTATCTCGAGTGTGTCTC CGGGACCAGCACTACTACTCCTATGTGGAGTTGCCTCTGGCCTGCGAAGGTGGCCGCTACGGGCTGATC CAGGCTGCAGCTGTGGCCACGTCCAGGGAGGTGGCGCATGGGGAGGTGCTCTTTGCAGCTTTCTCCTCG GCTGCACCCCCCACTGTGGGCCGGCCCCCATCGGCGGCTGCTGGGGCATCTGGAGCCTCTGCCCTCTGT GCCTTCCCCCTGGATGAGGTGGACCGGCTTGCTAATCGCACGCGAGATGCCTGCTACACCCGGGAGGGT CGTGCTGAGGATGGGACCGAGGTGGCCTACATCGAGTATGATGTCAATTCTGACTGTGCACAGCTGCCA GTGGACACCCTGGATGCTTATCCCTGTGGCTCAGACCACACGCCCAGCCCCATGGCCAGCCGGGTCCCG CTGGAAGCCACACCAATTCTGGAGTGGCCAGGGATTCAGCTAACAGCTGTGGCAGTCACCATGGAAGAT GGACACACCATCGCTTTCCTGGGTGATAGTCAAGGGCAGCTGCACAGGGTCTACTTGGGCCCAGGGAGC GATGGCCACCCATACTCCACACAGAGCATCCAGCAGGGGTCTGCAGTGAGCAGAGACCTCACCTTTGAT GGGACCTTTGAGCACCTGTATGTCATGACCCAGAGCACACTTCTGAAGGTTCCTGTGGCTTCCTGTGCT CAGCACCTGGACTGTGCATCTTGCCTTGCTCACAGGGACCCATACTGTGGGTGGTGCGTGCTCCTTGGC AGGTGCAGTCGCCGTTCTGAGTGCTCGAGGGGCCAGGGCCCAGAGCAGTGGCTATGGAGCTTCCAGCCT GAGCTGGGCTGTCTGCAAGTGGCAGCCATGAGTCCTGCCAACATCAGCCGAGAGGAGACGAGGGAGGTT TTCCTATCAGTGCCAGACCTGCCACCCCTGTGGCCAGGGGAGTCATATTCCTGCCACTTTGGGGAACAT CAGAGTCCTGCCCTGCTGACTGGTTCTGGTGTGATGTGCCCCTCCCCAGACCCTAGTGAGGCCCCAGTG CTGCCGAGAGGAGCCGACTACGTATCCGTGAGCGTGGAGCTCAGATTTGGCGCTGTTGTGATCGCCAAA ACTTCCCTCTCTTTCTATGACTGTGTGGCGGTCACTGAACTCCGCCCATCTGCGCAGTGCCAGGCCTGT GTGAGCAGCCGCTGGGGGTGTAACTGGTGTGTCTGGCAGCACCTGTGCACCCACAAGGCCTCGTGTGAT GCTGGGCCCATGGTTGCAAGCCATCAGAGCCCGCTTGTCTCCCCAGACCCTCCTGCAAGAGGTGGACCC AGCCCCTCCCCACCCACAGCCCCCAAAGCCCTGGCCACCCCTGCTCCTGACACCCTTCCCGTGGAGCCT GGGGCTCCCTCCACAGCCACAGCTTCGGACATCTCACCTGGGGCTAGTCCTTCCCTGCTCAGCCCCTGG GGGCCATGGGCAGGTTCTGGCTCCATATCTTCCCCTGGCTCCACAGGGTCGCCTCTCCATGAGGAGCCC TCCCCTCCCAGCCCCCAAAATGGACCTGGAACCGCTGTCCCTGCCCCCACTGACTTCAGACCCTCAGCC ACACCTGAGGACCTCTTGGCCTCCCCGCTGTCACCCTCAGAGGTAGCAGCAGTGCCCCCTGCAGACCCT GGCCCCGAGGCTCTTCATCCCACAGTGCCCCTGGACCTGCCCCCTGCCACTGTTCCTGCCACCACTTTC CCAGGGGCCATGGGCTCCGTGAAGCCCGCCCTGGACTGGCTCACGAGAGAAGGCGGCGAGCTGCCCGAG GCGGACGAGTGGACGGGGGGTGACGCACCCGCCTTCTCCACTTCCACCCTCCTCTCAGGTGATGGAGAC TCAGCAGAGCTTGAGGGCCCTCCCGCCCCCCTCATCCTCCCGTCCAGCCTCGACTACCAGTATGACACC CCCGGGCTCTGGGAGCTGGAAGAGGCGACCTTGGGGGCAAGCTCCTGCCCCTGTGTGGAGAGCGTTCAG GGCTCCACGTTGATGCCGGTCCATGTGGAGCGGGAAATCCGGCTGCTAGGCAGGAACCTGCACCTTTTC CAGGATGGCCCAGGAGACAATGAGTGTGTGATGGAGCTGGAGGGCCTCGAGGTGGTGGTTGAGGCCCGG GTCGAGTGTGAGCCACCTCCAGATACCCAGTGCCATGTCACCTGCCAGCAGCACCAGCTCAGCTATGAG GCTCTGCAGCCGGAGCTCCGTGTGGGGCTGTTTCTGCGTCGGGCCGGCCGTCTGCGTGTGGACAGTGCT GAGGGGCTGCATGTGGTACTGTATGACTGTTCCGTGGGACATGGAGACTGCAGCCGCTGCCAAACTGCC ATGCCCCAGTATGGCTGTGTGTGGTGTGAGGGGGAGCGTCCACGTTGTGTGACCCGGGAGGCCTGTGGT GAGGCTGAGGCTGTGGCCACCCAGTGCCCAGCGCCCCTCATCCACTCGGTGGAGCCACTGACTGGGCCT GTAGACGGAGGCACCCGTGTCACCATCAGGGGCTCCAACCTGGGCCAGCATGTGCAGGATGTGCTGGGC ATGGTCACGGTGGCTGGAGTGCCCTGTGCTGTGGATGCCCAGGAGTACGAGGTCTCCAGCAGCCTCGTG TGCATCACCGGGGCCAGTGGGGAGGAGGTGGCCGGCGCCACAGCGGTGGAGGTGCCGGGAAGAGGACGT GGTGTCTCAGAACACGACTTTGCCTACCAGGATCCGAAGGTCCATTCCATCTTCCCGGCCCGCGGCCCC AGAGCTGGGGGCACCCGTCTCACCCTGAATGGCTCCAAGCTCCTGACTGGGCGGCTGGAGGACATCCGA GTGGTGGTTGGAGACCAGCCTTGTCACTTGCTGCCGGAGCAGCAGTCAGAACAACTGCGGTGTGAGACC AGCCCACGCCCCACGCCTGCCACGCTCCCTGTGGCTGTGTGGTTTGGGGCCACGGAGCGGAGGCTTCAA CGCGGACAGTTCAAGTATACCTTGGACCCCAACATCACCTCTGCTGGCCCCACCAAGAGCTTCCTCAGT GGAGGACGTGAGATATGCGTCCGTGGCCAGAATCTGGACGTGGTACAGACGCCAAGAATCCGGGTGACC GTGGTCTCGAGAATGCTGCAGCCCAGCCAGGGGCTTGGACGGAGGCGTCGCGTGGTCCCGGAGACGGCA TGTTCCCTTGGACCCTCCTGCAGTAGCCAGCAATTTGAGGAGCCGTGCCATGTCAACTCCTCCCAGCTC ATCACGTGCCGCACACCTGCCCTCCCAGGCCTGCCTGAGGACCCCTGGGTCCGGGTGGAATTTATCCTT GACAACCTGGTCTTTGACTTTGCAACACTGAACCCCACACCTTTCTCCTATGAGGCCGACCCCACCCTG CAGCCACTCAACCCTGAGGACCCCACCATGCCATTCCGGCACAAGCCTGGGAGTGTGTTCTCCGTGGAG GGGGAGAACCTGGACCTTGCAATGTCCAAGGAGGAGGTGGTGGCTATGATAGGGGATGGCCCCTGTGTG GTGAAGACGCTGACGCGGCACCACCTGTACTGCGAGCCCCCCGTGGAGCAGCCCCTGCCACGGCACCAT GCCCTCCGAGAGGCACCTGACTCTTTGCCTGAGTTCACGGTGCAGATGGGGAACTTGCGCTTCTCCCTG GGTCACGTGCAGTATGACGGCGAGAGCCCTGGGGCTTTTCCTGTGGCAGCCCAGGTGGGCTTGGGGGTG GGCACCTCTCTTCTGGCTCTGGGTGTCATCATCATTGTCCTCATGTACAGGAGGAAGAGCAAGCAGGCC CTGAGGGACTATAAGAAGGTTCAGATCCAGCTGGAGAATCTGGAGAGCAGTGTGCGGGACCGCTGCAAG AAGGAATTCACAGACCTCATGACTGAGATGACCGATCTCACCAGTGACCTCCTGGGCAGCGGCATCCCC TTCCTCGACTACAAGGTGTATGCGGAGAGGATCTTCTTCCCTGGGCACCGCGAGTCGCCCTTGCACCGG GACCTGGGTGTGCCTGAGAGCAGACGGCCCACTGTGGAGCAAGGGCTGGGGCAGCTCTCTAACCTGCTC AACAGCAAGCTCTTCCTCACCAAGTTCATCCACACGCTGGAGAGCCAGCGCACCTTTTCAGCTCGGGAC CGTGCCTACGTGGCATCTCTGCTCACCGTGGCACTGCATGGGAAGCTTGAGTATTTCACTGACATCCTC CGCACTCTGCTCAGTGACCTGGTTGCCCAGTATGTGGCCAAGAACCCCAAGCTGATGCTGCGCAGGACA GAGACTGTGGTGGAGAAGCTGCTCACCAACTGGATGTCCATCTGTCTGTATACCTTCGTGAGGGACTCC GTAGGGGAGCCTCTGTACATGCTCTTTCGAGGGATTAAGCACCAAGTGGATAAGGGGCCAGTGGACAGT GTGACAGGCAAGGCCAAATACACCTTGAACGACAACCGCCTGCTCAGAGAGGATGTGGAGTACCGTCCC CTGACCTTGAATGCACTATTGGCTGTGGGGCCTGGGGCAGGAGAGGCCCAGGGCGTGCCCGTGAAGGTC CTAGACTGTGACACCATCTCCCAGGCAAAGGAGAAGATGCTGGACCAGCTTTATAAAGGAGTGCCTCTC ACCCAGCGGCCAGACCCTCGCACCCTTGATGTTGAGTGGCGGTCTGGGGTGGCCGGGCACCTCATTCTT TCTGACGAGGATGTCACTTCTGAGGTCCAGGGTCTGTGGAGGCGCCTGAACACACTGCAGCATTACAAG GTCCCAGATGGAGCAACTGTGGCCCTCGTCCCCTGCCTCACCAAGCATGTGCTCCGGGAAAACCAGGAT TATGTCCCTGGAGAGCGGACCCCAATGCTGGAGGATGTAGATGAGGGGGGCATCCGGCCCTGGCACCTG GTGAAGCCAAGTGATGAGCCGGAGCCGCCCAGGCCTCGGAGGGGCAGCCTTCGGGGCGGGGAGCGTGAG CGCGCCAAGGCCATCCCTGAGATCTACCTGACCCGCCTGCTGTCCATGAAGGGCACCCTGCAGAAGTTC GTGGATGACCTGTTCCAGGTGATTCTCAGCACCAGCCGCCCCGTGCCGCTCGCTGTGAAGTACTTCTTT GACCTGCTGGATGAGCAGGCCCAGCAGCATGGCATCTCCGACCAGGACACCATCCACATCTGGAAGACC AACAGCTTGCCTCTGAGGTTCTGGATCAATATAATAAAAAACCCGCAGTTTGTGTTCGACGTGCAAACA TCTGATAACATGGATGCGGTGCTCCTTGTCATTGCACAGACCTTCATGGACGCCTGCACCCTGGCCGAC CACAAGCTGGGCCGGGACTCCCCGATCAACAAACTTCTGTATGCACGGGACATTCCCCGGTACAAGCGG ATGGTGGAAAGGTACTATGCAGACATCAGACAGACTGTCCCAGCCAGCGACCAAGAGATGAACTCTGTC CTGGCTGAACTGTCCTGGAACTACTCCGGAGACCTCGGGGCGCGAGTGGCCCTGCATGAACTCTACAAG TACATCAACAAGTACTATGACCAGATCATCACTGCCCTGGAGGAGGATGGCACGGCCCAGAAGATGCAG CTGGGCTATCGGCTCCAGCAGATTGCAGCTGCTGTGGAAAACAAGGTCACAGATCTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_002673
Insert Size	6408 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002673.5
RefSeq Size	7325 bp
RefSeq ORF	6408 bp
Locus ID	5364
UniProt ID	O43157
Protein Families	Druggable Genome
Protein Pathways	Axon guidance
MW	232.3 kDa
Gene Summary	Receptor for SEMA4D (PubMed:19843518, PubMed:20877282, PubMed:21912513). Plays a role in GABAergic synapse development (By similarity). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed:12196628, PubMed:15210733). Plays a role in axon guidance, invasive growth and cell migration (PubMed:12198496).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.