Cripto1 (TDGF1) (NM_003212) Human Untagged Clone

CAT#: SC303284

TDGF1 (untagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 1



  "NM_003212" in other vectors (6)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal CRIPTO Antibody
    • 100 ul

CNY 5,381.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CR; CR-1; CRGF; CRIPTO
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003212 edited
AGTTTCCCCTGGACGCCTTGCTCCTGCTTCTGCTACGACCTTCTGGGGAAAACGAATTTC
TCATTTTCTTCTTAAATTGCCATTTTCGCTTTAGGAGATGAATGTTTTCCTTTGGCTGTT
TTGGCAATGACTCTGAATTAAAGCGATGCTAACGCCTCTTTTCCCCCTAATTGTTAAAAG
CTATGGACTGCAGGAAGATGGCCCGCTTCTCTTACAGTGTGATTTGGATCATGGCCATTT
CTAAAGCCTTTGAACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCAT
CTCGGGGATACCTGGCCTTCAGAGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTC
GGCCTCGGTCTTCCCAGCGTGTGCCGCCCATGGGGATACAGCACAGTAAGGAGCTAAACA
GAACCTGCTGCCTGAATGGGGGAACCTGCATGCTGGGGTCCTTTTGTGCCTGCCCTCCCT
CCTTCTACGGACGGAACTGTGAGCACGATGTGCGCAAAGAGAATTGTGGGTCTGTGCCCC
ATGACACCTGGCTGCCCAAGAAGTGTTCCCTGTGTAAATGCTGGCACGGTCAGCTCCGCT
GCTTTCCTCAGGCATTTCTACCCGGCTGTGATGGCCTTGTGATGGATGAGCACCTCGTGG
CTTCCAGGACTCCAGAACTACCACCGTCTGCGCGTACTACCACTTTTATGCTAGTTGGCA
TCTGCCTTTCTATACAAAGCTACTATTAATCGACATTGACCTATTTCCAGAAATACAATT
TTAGATATCATGCAAATTTCATGACCAGTAAAGGCTGCTGCTACAATGTCCTAACTGAAA
GATGATCATTTGTAGTTGCCTTAAAATAATGAATACATTTCCAAAATGGTCTCTAACATT
TCCTTACAGAACTACTTCTTACTTCTTTGCCCTGCCCTCTCCCAAAAAACTACTTCTTTT
TTCAAAAGAAAGTCAGCCATATCTCCATTGTGCCTAAGTCCAGTGTTTCTTTTTTTTTTT
TTTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGACTGCAATGACGCGATCTTGGTT
CACTGCAACCTCCGCATCCGGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCCAAGTAACT
GGGATTACAGGCATGTGTCACCATGCCCAGCTAATTTTTTTGTATTTTTAGTAGAGATGG
GGGTTTCACCATATTGGCCAGTCTGGTCTCGAACTCCTGACCTTGTGATCCACTCGCCTC
AGCCTCTCGAAGTGCTGAGATTACACACGTGAGCAACTGTGCAAGGCCTGGTGTTTCTTG
ATACATGTAATTCTACCAAGGTCTTCTTAATATGTTCTTTTAAATGATTGAATTATATGT
TCAGATTATTGGAGACTAATTCTAATGTGGACCTTAGAATACAGTTTTGAGTAGAGTTGA
TCAAAATCAATTAAAATAGTCTCTTTAAAAGGAAAGAAAACATCTTTAAGGGGAGGAACC
AGAGTGCTGAAGGAATGGAAGTCCATCTGCGTGTGTGCAGGGAGACTGGGTAGGAAAGAG
GAAGCAAATAGAAGAGAGAGGTTGAAAAACAAAATGGGTTACTTGATTGGTGATTAGGTG
GTGGTAGAGAAGCAAGTAAAAAGGCTAAATGGAAGGGCAAGTTTCCATCATCTATAGAAA
GCTATATAAGACAAGAAATCCCCTTTTTTTCCCAAAGGCAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
>OriGene 5' read for NM_003212 unedited
AGCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGA
GTTTCCCCTGGACGCCTTGCTCCTGCTTCTGCTACGACCTTCTGGGGAAAACGAATTTCT
CATTTTCTTCTTAAATTGCCATTTTCGCTTTAGGAGATGAATGTTTTCCTTTGGCTGTTT
TGGCAATGACTCTGAATTAAAGCGATGCTAACGCCTCTTTTCCCCCTAATTGTTAAAAGC
TATGGACTGCAGGAAGATGGCCCGCTTCTCTTACAGTGTGATTTGGATCATGGCCATTTC
TAAAGCCTTTGAACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCATC
TCGGGGATACCTGGCCTTCAGAGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTCG
GCCTCGGTCTTCCCAGCGTGTGCCGCCCATGGGGATACAGCACAGTAAGGAGCTAAACAG
AACCTGCTGCCTGAATGGGGGAACCTGCATGCTGGGGTCCTTTTGTGCCTGCCCTCCCTC
CTTCTACGGACGGAACTGTGAGCACGATGTGCGCAAAGAGAATTGTGGGTCTGTGCCCCA
TGACACCTGGCTGCCCNAGAAGTGTTCCCTGTGTAAATGCTGGCACGGTCAGCTCCGCTG
CTTTTCTCAGGCATTTCTACCCGGCTGTGATGCCCTTGTGATGGATGAGCACCTCGTTGC
TTTCAGGACTCCAGAACTAACAACGTCTGCGCGTACTACCACTTTTATGCTAGTTGGCAT
CTGCCTTTCCTTTTCAAAGCTACTATTTAATCGACATTGACCTCATTCCCCGAAATACAT
TTTAGATATCATGGCAATTTCTTGAACCGTAAAGGCCTGCTGCTCAATGGCCTTACTGGA
AGAGGATCATTTTGAGGTTGCCTTAAATAAGGAAACCTTTCCCAAATGGCT
Restriction Sites Please inquire     
ACCN NM_003212
Insert Size 1800 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 1 aa.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003212.1, NP_003203.1
RefSeq Size 2033 bp
RefSeq ORF 567 bp
Locus ID 6997
UniProt ID P13385
Protein Families Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
Gene Summary This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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