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CEBP Alpha (CEBPA) (NM_004364) Human Untagged Clone
CAT＃: SC303472
CEBPA (untagged)-Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA)
CNY 3,656.00
CNY 5,800.00
| Cited in 7 publications. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C/EBP-alpha; CEBP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC303472 sequence for NM_004364 edited (data generated by NextGen Sequencing)
ATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCCGATGAGCAGCCACCTGCAG AGCCCCCCGCACGCGCCCAGCAGCGCCGCCTTCGGCTTTCCCCGGGGCGCGGGCCCCGCG CAGCCTCCCGCCCCACCTGCCGCCCCGGAGCCGCTGGGCGGCATCTGCGAGCACGAGACG TCCATCGACATCAGCGCCTACATCGACCCGGCCGCCTTCAACGACGAGTTCCTGGCCGAC CTGTTCCAGCACAGCCGGCAGCAGGAGAAGGCCAAGGCGGCCGTGGGCCCCACGGGCGGC GGCGGCGGCGGCGACTTTGACTACCCGGGCGCGCCCGCGGGCCCCGGCGGCGCCGTCATG CCCGGGGGAGCGCACGGGCCCCCGCCCGGCTACGGCTGCGCGGCCGCCGGCTACCTGGAC GGCAGGCTGGAGCCCCTGTACGAGCGCGTCGGGGCGCCGGCGCTGCGGCCGCTGGTGATC AAGCAGGAGCCCCGCGAGGAGGATGAAGCCAAGCAGCTGGCGCTGGCCGGCCTCTTCCCT TACCAGCCGCCGCCGCCGCCGCCGCCCTCGCACCCGCACCCGCACCCGCCGCCCGCGCAC CTGGCCGCCCCGCACCTGCAGTTCCAGATCGCGCACTGCGGCCAGACCACCATGCACCTG CAGCCCGGTCACCCCACGCCGCCGCCCACGCCCGTGCCCAGCCCGCACCCCGCGCCCGCG CTCGGTGCCGCCGGCCTGCCGGGCCCTGGCAGCGCGCTCAAGGGGCTGGGCGCCGCGCAC CCCGACCTCCGCGCGAGTGGCGGCAGCGGCGCGGGCAAGGCCAAGAAGTCGGTGGACAAG AACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAACAACATCGCGGTGCGCAAGAGCCGC GACAAGGCCAAGCAGCGCAACGTGGAGACGCAGCAGAAGGTGCTGGAGCTGACCAGTGAC AATGACCGCCTGCGCAAGCGGGTGGAACAGCTGAGCCGCGAACTGGACACGCTGCGGGGC ATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCAAGGCCATGGGCAACTGCGCGTGA Clone variation with respect to NM_004364.3 >OriGene 5' read for NM_004364 unedited
CCCTTATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCGGCCCCCGATGAGCAGCCACC TGCAGAGCCCCCCGCACGCGCCCAGCAGCGCCGCCTTCGGCTTTCCCCGGGGCGCGGGCC CCGCGCAGCCTCCCGCCCCACCTGCCGCCCCGGAGCCGCTGGGCGGCATCTGCGAGCACG AGACGTCCATCGACATCAGCGCCTACATCGACCCGGCCGCCTTCAACGACGAGTTCCTGG CCGACCTGTTCCAGCACAGCCGGCAGCAGGAGAAGGCCAAGGCGGCCGTGGCCCCACGGG CGG >OriGene 3' read for NM_004364 unedited
GTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACC AGATCTGAATTCGCCCTTGCACCGGAATCTCCTAGTCCTGGCTCGCACGGCTCGGGCAAG CCTCGAGATCCGGCGACCCCAAACCACTCCCTGGGTCCCCGCCGGAGGCTGGCCCAGGGC GGTCCCACAGCCGCGCGCCTCACGCGCAGTTGCCCATGGCCTTGACCAAGGAGCTCTCTG GCAGCTGGCGGAAGATGCCCCGCAGCGTGTCCAGTTCGCGGCTCAGCTGTTCCACCCGCT TGCGCAGGCGGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC GCTGCTTGGCCTTGTCGCGGCTCTTGCGCACCGCGATGTTGTTGCGCTCGCGCCGCACCC GGTACTCGTTGCTGTTCTTGTCCACCGACTTCTTGGCCTTGCCCGCGCCGCTGCCGCCAC TCGCGCGGAGGTCGGGGTGCGCGGCGCCCAGCCCCTTGAGCGCGCTGCCAGGGCCCGGCA GGCCGGCGGCACCGAGCGCGGGCGCGGGGTGCGGGCTGGGCACGGGCGTGGGCGGCGGCG TGGGGTGACCGGGCTGCAGGTGCATGGTGGTCTGGCCGCAGTGCGCGATCTGGAACTGCA GGTGCGGGGCGGCCAGGTGCGCGGGCGGCGGGTGCGGGTGCGGTCCAAAAGGGCGGNNNN NNNNNNNNNNNNNNNN |
| Restriction Sites | Please inquire |
| ACCN | NM_004364 |
| Insert Size | 1077 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004364.2, NP_004355.2 |
| RefSeq Size | 2385 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 1050 |
| UniProt ID | P49715 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
| Protein Pathways | Acute myeloid leukemia, Pathways in cancer |
| Gene Summary | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (a, also known as C/EBP-42) represented in this RefSeq results from translation initiation at the first AUG start codon. Isoform a has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Evaluation of human dermal fibroblasts directly reprogrammed to adipocyte-like cells as a metabolic disease model
,Chen, JH;Goh, KJ;Rocha, N;Groeneveld, MP;Minic, M;Barrett, TG;Savage, D;Semple, RK;,
Dis Model Mech
,PubMed ID 28982679
[CEBPA]
|
|
Roles for Transcription Factors Sp1, NF-?B, IRF3, and IRF7 in Expression of the Human IFNL4 Gene
,Chinnaswamy, S;Bhushan, A;Behera, AK;Ghosh, S;Rampurkar, V;Chandra, V;Pandit, B;Kundu, TK;,
Viral Immunol.
,PubMed ID 26684959
[CEBPA]
|
|
Role ofTranscription Factor CCAAT/Enhancer Binding Protein Alphain Human Fetal LiverCell Typesin vitro
,Gerlach, JC;Over, P;Foka, HG;Turner, ME;Thompson, RL;Gridelli, B;Schmelzer, E;,
Hepatol. Res.
,PubMed ID 25195540
[CEBPA]
|
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Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs
,Simeonov, KP;Uppal, H;,
PLoS ONE
,PubMed ID 24963715
[CEBPA]
|
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The CCAAT/Enhancer-Binding Protein Beta-2 Isoform (CEBPß-2) Upregulates Galectin-7 Expression in Human Breast Cancer Cells
,Campion, CG;Labrie, M;Grosset, AA;St-Pierre, Y;,
PLoS ONE
,PubMed ID 24789216
[CEBPA]
|
|
PU.1 and C/EBPa synergistically program distinct response to NF-{kappa}B activation through establishing monocyte specific enhancers
,Fulai Jin, Yan Li, Bing Ren, and Rama Natarajan,
PNAS, Mar 2011; 108: 5290 - 5295
[CEBPA]
|
|
Reintroduction of CEBPA in MN1-overexpressing hematopoietic cells prevents their hyper proliferation and restores myeloid differentiation
,Ayten Kandilci and Gerard C. Grosveld,
Blood, Jun 2009; 10.1182/blood-2009-02-205443.
[CEBPA]
|
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218955 | CEBPA (Myc-DDK-tagged)-Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA) |
CNY 5,488.00 |
|
| RC218955L1 | Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC218955L2 | Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), mGFP tagged |
CNY 7,888.00 |
|
| RC218955L3 | Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC218955L4 | Lenti ORF clone of Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), mGFP tagged |
CNY 7,888.00 |
|
| RG218955 | CEBPA (tGFP-tagged) - Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA) |
CNY 7,088.00 |
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