KRT75 (NM_004693) Human Untagged Clone

CAT#: SC303521

KRT75 (untagged)-Human keratin 75 (KRT75)



  "NM_004693" in other vectors (4)

CNY 8,840.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-KRT75 Antibody - middle region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CK-75; hK6hf; K6HF; K75; KB18; PFB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC303521 representing NM_004693.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTCGGCAGTCCTCCATCACCTTCCAGTCTGGCAGCCGCAGGGGCTTCAGCACCACCTCGGCCATC
ACCCCGGCAGCTGGCCGCTCCCGCTTCAGCTCTGTCTCTGTGGCCCGCTCTGCAGCAGGGAGTGGGGGC
CTGGGAAGGATCAGCAGTGCTGGGGCCAGCTTTGGAAGCCGCAGCCTCTACAACCTGGGGGGTGCCAAG
CGGGTCTCCATCAATGGGTGTGGCAGCAGCTGCCGAAGTGGCTTTGGTGGCAGGGCCAGCAACAGGTTT
GGAGTCAACAGTGGATTTGGCTATGGGGGTGGAGTTGGAGGAGGCTTCAGTGGCCCCAGCTTCCCCGTG
TGTCCCCCTGGAGGCATCCAAGAGGTCACTGTCAACCAGAGTCTCCTGACTCCTCTTCACCTGCAAATC
GACCCCACCATCCAGCGGGTGCGGGCCGAGGAGCGCGAGCAGATCAAGACCCTCAACAATAAGTTCGCC
TCCTTCATCGACAAGGTGAGGTTCTTGGAGCAGCAGAACAAGGTCCTGGAGACCAAGTGGGCCCTCCTG
CAGGAGCAGGGCTCCAGGACTGTGAGGCAGAACCTAGAGCCCCTCTTTGATTCCTATACCAGTGAGCTC
CGACGGCAGCTGGAAAGCATCACCACCGAGAGGGGCAGGCTTGAAGCTGAACTGAGGAACATGCAGGAT
GTTGTGGAAGATTTCAAAGTCAGGTACGAAGATGAAATTAACAAGCGCACAGCTGCTGAGAATGAATTT
GTAGCCCTGAAAAAGGACGTAGATGCTGCCTATATGAACAAGGTGGAGCTGGAAGCCAAGGTCAAATCT
CTGCCCGAGGAGATCAACTTCATCCACTCAGTCTTTGATGCAGAGCTGTCCCAGTTGCAGACCCAGGTC
GGTGACACATCCGTGGTGCTGTCCATGGACAACAACCGCAACCTGGACCTGGATAGTATCATCGCCGAG
GTCAAAGCACAATACGAGGACATTGCCAACCGCAGCCGGGCCGAGGCTGAGTCCTGGTACCAGACCAAG
TACGAGGAGCTGCAGGTCACCGCAGGCAGACATGGGGATGACCTTCGAAACACCAAACAAGAGATCTCT
GAAATGAACCGCATGATCCAGAGGCTGAGAGCTGAGATTGACAGCGTCAAGAAGCAGTGTTCCAGCTTG
CAAACGGCCATTGCTGATGCAGAGCAGCGGGGAGAACTGGCTCTCAAGGATGCACGGGCCAAGCTGGTG
GACCTTGAGGAGGCCCTGCAGAAGGCCAAGCAGGACATGGCTCGGCTCCTGCGTGAGTACCAGGAGCTG
ATGAACATCAAGCTGGCCCTGGACGTGGAGATCGCCACCTACCGCAAGCTGCTGGAAGGCGAGGAGTGC
AGGTTGAGTGGAGAGGGAGTTTCTCCAGTTAACATTTCTGTGGTCACCTCTACTCTTTCCAGTGGCTAT
GGAAGCGGCAGCAGCATTGGAGGTGGAAACCTGGGCCTCGGTGGGGGCAGCGGCTACTCCTTCACCACC
AGTGGTGGGCATAGCCTGGGTGCAGGCCTGGGAGGTTCTGGATTCAGTGCCACCAGCAACCGGGGCTTA
GGGGGCAGTGGTTCTAGCGTCAAGTTTGTCTCCACCACATCCTCCAGCCAGAAGAGCTACACGCACTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_004693
Insert Size 1656 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004693.2
RefSeq Size 2125 bp
RefSeq ORF 1656 bp
Locus ID 9119
UniProt ID O95678
MW 59.6 kDa
Gene Summary This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. This gene is expressed in the companion layer, upper germinative matrix region of the hair follicle, and medulla of the hair shaft. The encoded protein plays an essential role in hair and nail formation. Variations in this gene have been associated with the hair disorders pseudofolliculitis barbae (PFB) and loose anagen hair syndrome (LAHS). [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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