PRAX 1 (TSPOAP1) (NM_004758) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BZRAP1; PBR-IP; PRAX-1; PRAX1; RIM-BP1; RIMBP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303530 representing NM_004758. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCAACTGACAACCCTCCCACGGCCTGGGGACCCTGGAGCCATGGAGCCATGGGCACTGCCCACC TGGCATAGCTGGACTCCAGGTCGAGGGGGTGAACCTAGCAGTGCAGCCCCAAGCATCGCTGATACTCCT CCGGCAGCTCTGCAGCTTCAAGAACTGAGGTCTGAGGAGAGTTCCAAGCCCAAAGGAGACGGGAGCTCC AGGCCCGTGGGGGGAACTGACCCTGAAGGAGCAGAGGCTTGTCTGCCCAGCCTGGGCCAGCAAGCATCC AGCTCTGGACCCGCCTGCCAGAGGCCAGAGGATGAGGAAGTGGAGGCTTTCCTGAAGGCCAAGCTGAAT ATGAGCTTTGGGGACAGGCCCAATCTGGAGCTGCTGAGGGCCCTGGGGGAGCTGCGGCAGCGCTGTGCC ATCCTTAAGGAGGAAAACCAGATGCTGAGGAAGAGCAGCTTCCCTGAGACAGAAGAGAAGGTGCGGAGG CTGAAGAGGAAGAACGCCGAGCTGGCGGTCATTGCCAAGCGCCTGGAGGAGAGGGCCCGAAAGCTGCAG GAAACGAACCTGAGGGTGGTGAGTGCCCCCTTGCCCCGGCCGGGGACCAGCTTGGAGTTGTGTCGGAAG GCCCTAGCCCGCCAGCGAGCCCGGGACCTCAGTGAGACAGCCAGTGCACTGCTGGCCAAGGACAAGCAG ATTGCTGCCTTGCAGCGGGAGTGCAGGGAGCTGCAGGCCAGGCTCACTCTGGTGGGCAAGGAGGGTCCC CAGTGGCTCCACGTGCGGGACTTCGATCGGCTGCTGCGCGAGTCCCAGCGGGAGGTGCTGCGGCTGCAG AGGCAGATCGCGCTGCGCAACCAGCGGGAGACGCTCCCGCTCCCGCCGTCCTGGCCCCCGGGCCCTGCT CTCCAGGCCAGAGCAGGGGCGCCTGCTCCCGGGGCCCCGGGAGAGGCCACGCCCCAGGAGGATGCGGAC AACCTACCCGTGATTCTAGGGGAGCCAGAGAAAGAGCAGAGGGTGCAGCAGCTGGAATCGGAGCTCAGC AAGAAGCGGAAGAAATGCGAGAGCCTGGAGCAGGAAGCCCGGAAAAAGCAGAGGCGATGTGAGGAGCTG GAACTGCAGCTGAGACAAGCGCAGAATGAGAATGCCCGCCTGGTGGAGGAGAACTCCCGGCTCAGTGGG AGAGCCACAGAGAAGGAGCAGGTGGAGTGGGAGAATGCGGAGCTGAGGGGCCAGCTCCTGGGGGTGACA CAGGAGAGGGACTCAGCCCTTCGCAAGAGCCAGGGCCTGCAGAGCAAGCTGGAGAGCCTGGAGCAAGTG CTGAAGCACATGCGGGAGGTGGCCCAGCGGCGGCAGCAGCTGGAGGTGGAGCATGAACAGGCTCGGCTC AGCCTACGGGAGAAGCAGGAGGAGGTCCGGAGACTGCAGCAGGCCCAGGCTGAAGCCCAGAGGGAACAT GAAGGAGCCGTGCAGCTGCTGGAGTCTACCTTGGATTCCATGCAGGCCCGGGTTCGAGAGCTCGAAGAA CAGTGCCGCAGCCAAACCGAGCAGTTCAGCCTCCTGGCACAGGAACTCCAGGCTTTCCGCCTGCACCCG GGCCCCTTGGATCTGCTCACATCTGCCCTGGACTGTGGGAGCCTTGGAGACTGCCCACCACCCCCCTGC TGCTGCTCCATTCCCCAGCCTTGCCGGGGGTCTGGCCCCAAAGACCTTGACCTCCCGCCGGGCTCCCCT GGGCGCTGCACCCCAAAGTCTTCCGAGCCTGCCCCTGCCACTCTCACTGGGGTCCCTCGAAGGACAGCC AAGAAGGCAGAGTCTCTCTCCAACTCCTCCCACTCCGAGTCCATCCACAACAGCCCCAAGTCATGCCCT ACACCTGAGGTGGACACAGCCAGTGAGGTAGAGGAGCTGGAGGCAGACAGTGTCTCCCTGCTCCCAGCT GCGCCAGAGGGCAGCCGGGGAGGAGCCAGGATCCAGGTCTTCCTAGCACGTTATAGCTACAACCCCTTT GAGGGTCCCAATGAGAATCCAGAAGCAGAGCTTCCGCTGACAGCTGGCGAGTACATCTACATCTATGGC AACATGGATGAGGATGGCTTTTTTGAAGGAGAGCTCATGGATGGCCGAAGGGGCCTGGTCCCTTCCAAT TTTGTAGAGCGTGTGTCGGATGATGACCTCCTGACCTCCCTCCCTCCAGAGCTGGCCGATTTGTCCCAC AGCTCAGGCCCTGAACTCAGTTTCCTGAGTGTAGGTGGGGGTGGCAGCAGTAGCGGGGGCCAAAGCAGT GTGGGAAGGAGCCAGCCCAGACCTGAGGAGGAGGATGCAGGGGACGAGCTCAGTCTGAGCCCATCACCG GAGGGCCTGGGCGAGCCTCCTGCCGTGCCTTACCCCCGCCGTCTGGTGGTCCTCAAGCAGCTGGCCCAC AGCGTGGTGCTGGCCTGGGAGCCGCCTCCTGAGCAAGTGGAGCTACACGGCTTCCATATCTGTGTGAAT GGGGAGCTGCGACAGGCCCTGGGGCCTGGGGCGCCACCCAAGGCCGTGCTGGAGAACCTGGACCTGTGG GCCGGGCCCCTTCACATTTCTGTCCAGGCCCTGACTAGCCGGGGCAGCTCTGACCCACTGCGCTGTTGC TTGGCGGTGGGTGCCCGGGCCGGAGTGGTGCCCAGCCAGCTGCGGGTCCATCGGTTGACAGCCACATCT GCTGAGATCACCTGGGTGCCCGGCAATAGCAACTTGGCCCATGCCATCTACCTCAATGGGGAAGAGTGC CCACCTGCCAGCCCCAGTACCTACTGGGCCACCTTCTGCCACTTACGGCCTGGCACACCCTATCAGGCC CAAGTGGAGGCTCAGCTCCCACCCCAAGGGCCCTGGGAACCAGGCTGGGAGAGGCTGGAGCAGCGGGCT GCCACCCTGCAGTTCACCACACTCCCAGCAGGCCCACCTGATGCCCCTCTGGATGTGCAGATCGAGCCT GGGCCCTCCCCTGGGATCTTGATCATCAGTTGGCTCCCAGTCACCATCGATGCTGCTGGCACATCCAAC GGTGTCCGGGTCACAGGCTATGCCATCTACGCTGATGGGCAGAAGATCATGGAGGTGGCCTCACCCACG GCAGGCAGTGTACTGGTGGAGTTGTCCCAGCTGCAGCTGCTGCAGGTGTGTCGTGAGGTGGTCGTGCGC ACCATGTCGCCCCACGGGGAGTCGGCGGACTCCATCCCGGCTCCTATCACTCCCGCCCTGGCTCCGGCC AGCCTGCCAGCCCGAGTCTCCTGCCCCTCACCGCACCCAAGCCCAGAGGCCAGAGCGCCCCTTGCTTCA GCCTCCCCAGGGCCTGGAGACCCCAGCTCTCCTCTCCAGCACCCTGCTCCCCTTGGAACTCAAGAGCCT CCAGGAGCACCCCCTGCAAGCCCTTCCAGAGAGATGGCAAAAGGGTCCCACGAGGACCCTCCAGCACCT TGCTCCCAGGAGGAGGCTGGGGCAGCAGTGCTGGGCACCTCAGAGGAGAGGACAGCCAGCACATCTACC CTGGGTGAGAAGGACCCTGGCCCCGCAGCTCCCTCACTGGCCAAGCAGGAGGCCGAGTGGACTGCAGGA GAGGCCTGTCCGGCCTCCAGCTCCACCCAGGGAGCACGGGCCCAGCAGGCGCCAAATACCGAGATGTGC CAAGGAGGAGACCCAGGGTCTGGGCTGAGGCCCAGGGCTGAGAAGGAGGACACAGCAGAGCTTGGGGTT CATCTGGTGAACTCCCTCGTGGACCACGGCCGCAACTCAGACCTGTCAGACATCCAGGAGGAAGAGGAA GAGGAGGAGGAGGAGGAGGAAGAGGAGCTGGGTTCCAGGACTTGCTCCTTCCAGAAGCAGGTTGCTGGC AACAGCATCAGGGAGAATGGGGCCAAGTCCCAGCCCGACCCCTTTTGTGAGACTGACAGCGATGAGGAG ATCTTGGAGCAGATCCTGGAGCTGCCCCTCCAGCAGTTCTGTAGCAAGAAGCTCTTTAGCATCCCGGAG GAGGAGGAAGAGGAAGAGGAGGACGAGGAGGAGGAGAAGTCAGGGGCAGGCTGTTCTTCCCGAGACCCT GGCCCGCCTGAACCTGCATTGCTGGGGCTGGGCTGTGACAGTGGTCAGCCCCGAAGACCTGGCCAGTGT CCCTTGTCTCCTGAGTCCTCCAGGGCTGGAGACTGCCTGGAAGACATGCCTGGATTAGTTGGTGGAAGC AGCCGGAGGAGAGGAGGGGGCTCCCCTGAGAAGCCCCCAAGCCGCAGGCGGCCTCCAGATCCCCGAGAA CACTGCAGCCGACTTCTCAGCAACAATGGGCCCCAGGCCTCTGGACGACTGGGCCCCACACGGGAGAGG GGTGGCCTCCCCGTAATTGAGGGCCCCAGGACTGGACTAGAGGCTAGCGGGAGAGGCCGGCTGGGCCCT TCCCGGAGGTGCTCCCGTGGCCGGGCGCTGGAGCCTGGCCTGGCCAGCTGCCTTTCCCCCAAGTGCTTG GAAATCAGCATTGAATATGATTCGGAGGATGAGCAGGAGGCGGGCAGCGGGGGCATCAGCATCACCAGC TCCTGCTACCCTGGAGATGGGGAGGCCTGGGGCACAGCAACTGTAGGAAGGCCCAGGGGGCCTCCGAAG GCCAATTCAGGCCCCAAGCCCTACCCACGCCTCCCAGCCTGGGAGAAAGGGGAACCAGAGCGGAGAGGC CGCAGTGCGACGGGCAGAGCCAAGGAGCCACTCTCCCGGGCAACAGAGACCGGAGAGGCCAGAGGGCAG GACGGCTCTGGGCGGAGGGGCCCCCAGAAGAGAGGTGTCCGAGTCCTCAGGCCAAGCACTGCAGAGCTA GTCCCTGCGAGGAGCCCCTCAGAAACACTGGCTTACCAGCACCTACCCGTCAGGATCTTTGTGGCTCTG TTTGACTATGACCCCGTGTCAATGTCGCCCAATCCTGATGCTGGAGAAGAAGAGCTTCCCTTCCGAGAG GGTCAGATCCTGAAGGTGTTTGGGGACAAGGATGCCGATGGCTTCTACCAGGGCGAAGGTGGGGGCCGG ACAGGCTACATTCCCTGCAACATGGTGGCTGAGGTGGCTGTGGACAGCCCTGCTGGGAGACAGCAACTG CTCCAGCGGGGTTATTTGTCCCCAGATATTCTCCTTGAGGGCTCAGGGAATGGTCCGTTTGTGTACTCC ACAGCCCACACAACTGGGCCTCCTCCCAAGCCCCGCCGCTCCAAGAAAGCTGAGTCGGAAGGCCCTGCC CAGCCCTGTCCAGGCCCCCCTAAGCTGGTCCCCTCTGCTGACCTGAAAGCTCCCCACTCCATGGTGGCT GCATTTGACTACAACCCCCAGGAGAGTTCCCCCAATATGGACGTGGAGGCAGAGCTGCCCTTCCGGGCA GGGGATGTCATTACTGTGTTTGGGGGCATGGACGATGACGGTTTCTACTATGGGGAATTAAATGGACAA AGGGGCCTGGTTCCATCCAACTTCCTGGAGGGCCCTGGGCCTGAGGCAGGCGGCCTGGACAGGGAACCC AGGACACCCCAGGCTGAGAGTCAGAGAACGAGGAGGAGAAGAGTCCAGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_004758
Insert Size	5574 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004758.3
RefSeq Size	7698 bp
RefSeq ORF	5574 bp
Locus ID	9256
UniProt ID	O95153
Protein Families	Druggable Genome
MW	200.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.