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WNT1 (NM_005430) Human Untagged Clone
CAT＃: SC303644
WNT1 (untagged)-Human wingless-type MMTV integration site family, member 1 (WNT1)
CNY 3,656.00
CNY 7,220.00
| Cited in 4 publications. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BMND16; INT1; OI15 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC303644 representing NM_005430.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGCTCTGGGCGCTGTTGCCTGGCTGGGTTTCTGCTACGCTGCTGCTGGCGCTGGCCGCTCTGCCC GCAGCCCTGGCTGCCAACAGCAGTGGCCGATGGTGGGGTATTGTGAACGTAGCCTCCTCCACGAACCTG CTTACAGACTCCAAGAGTCTGCAACTGGTACTCGAGCCCAGTCTGCAGCTGTTGAGCCGCAAACAGCGG CGTCTGATACGCCAAAATCCGGGGATCCTGCACAGCGTGAGTGGGGGGCTGCAGAGTGCCGTGCGCGAG TGCAAGTGGCAGTTCCGGAATCGCCGCTGGAACTGTCCCACTGCTCCAGGGCCCCACCTCTTCGGCAAG ATCGTCAACCGAGGCTGTCGAGAAACGGCGTTTATCTTCGCTATCACCTCCGCCGGGGTCACCCATTCG GTGGCGCGCTCCTGCTCAGAAGGTTCCATCGAATCCTGCACGTGTGACTACCGGCGGCGCGGCCCCGGG GGCCCCGACTGGCACTGGGGGGGCTGCAGCGACAACATTGACTTCGGCCGCCTCTTCGGCCGGGAGTTC GTGGACTCCGGGGAGAAGGGGCGGGACCTGCGCTTCCTCATGAACCTTCACAACAACGAGGCAGGCCGT ACGACCGTATTCTCCGAGATGCGCCAGGAGTGCAAGTGCCACGGGATGTCCGGCTCATGCACGGTGCGC ACGTGCTGGATGCGGCTGCCCACGCTGCGCGCCGTGGGCGATGTGCTGCGCGACCGCTTCGACGGCGCC TCGCGCGTCCTGTACGGCAACCGCGGCAGCAACCGCGCTTCGCGGGCGGAGCTGCTGCGCCTGGAGCCG GAAGACCCGGCCCACAAACCGCCCTCCCCCCACGACCTCGTCTACTTCGAGAAATCGCCCAACTTCTGC ACGTACAGCGGACGCCTGGGCACAGCAGGCACGGCAGGGCGCGCCTGTAACAGCTCGTCGCCCGCGCTG GACGGCTGCGAGCTGCTCTGCTGCGGCAGGGGCCACCGCACGCGCACGCAGCGCGTCACCGAGCGCTGC AACTGCACCTTCCACTGGTGCTGCCACGTCAGCTGCCGCAACTGCACGCACACGCGCGTACTGCACGAG TGTCTGTGA >OriGene 5' read for NM_005430 unedited
GTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCAACTGCAGCACA GAGCGGGCAAAGCCAGGCAGGCCATGGGGCTCTGGGCGCTGTTGCCTGGCTGGGTTTCTG CTACGCTGCTGCTGGCGCTGGCCGCTCTGCCCGCAGCCCTGGCTGCCAACAGCAGTGGCC GATGGTGGGGTATTGTGAACGTAGCCTCCTCCACGAACCTGCTTACAGACTCCAAGAGTC TGCAACTGGTACTCGAGCCCAGTCTGCAGCTGTTGAGCCGCAAACAGCGGCGTCTGATAC GCCAAAATCCGGGGATCCTGCACAGCGTGAGTGGGGGGCTGCAGAGTGCCGTGCGCGAGT GCAAGTGGCAGTTCCGGAATCGCCGCTGGAACTGTCCCACTGCTCCAGGGCCCCACCTCT TCGGCAAGATCGTCAACCGAGGCTGTCGAGAAACGGCGTTTATCTTCGCTATCACCTCCG CCGGGGTCACCCATTCGGTGGCGCGCTCCTGCTCAGAAGGTTCCATCGAATCCTGCACGT GTGACTACCGGCGGCGCGGCCCCGGGGGCCCCGACTGGCACTGGGGGGGCTGCAGCGACA ACATTGACTTCGGCCGCCTCTTCGGCCCGGAGTTCGTGGACTCCGGGGAGAAAGGGCGGG ACCTGCGCTTCCTCATGAACCTTTCACAACACGAGGCAAGCCGTACGACCGTATTCTCCG AGATGCGGCAGGAGTGCAAGTGCCACGGGATGTCCGGCTCATGCACCGTGCGCACTTGCT GGATGCCGGTGCCCACGCTGCGCGCCTTGGGCCATTTGCTTGCCCAACCGTTTTAACGGG GCTTGGGCGTCCTGGACGGGAAACCGGGGAAGAAACCGGCCTTG |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_005430 |
| Insert Size | 1113 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005430.2 |
| RefSeq Size | 2368 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 7471 |
| UniProt ID | P04628 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
| MW | 41 kDa |
| Gene Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Novel WNT1 mutations in children with osteogenesis imperfecta: Clinical and functional characterization
,Lu, Y;Ren, X;Wang, Y;Bardai, G;Sturm, M;Dai, Y;Riess, O;Zhang, Y;Li, H;Li, T;Zhai, N;Zhang, J;Rauch, F;Han, J;,
Bone
,PubMed ID 29935254
[WNT1]
|
|
Skeletal characteristics associated with homozygous and heterozygous WNT1 mutations
,Palomo, T;Al-Jallad, H;Moffatt, P;Glorieux, FH;Lentle, B;Roschger, P;Klaushofer, K;Rauch, F;,
Bone July 2014
,PubMed ID 25010833
[WNT1]
|
|
EMILIN2 Down-modulates the Wnt Signalling Pathway and Suppresses Breast Cancer Cell Growth and Migration
,Marastoni, S;Andreuzzi, E;Paulitti, A;Colladel, R;Pellicani, R;Todaro, F;Schiavinato, A;Bonaldo, P;Colombatti, A;Mongiat, M;,
J. Pathol. Dec 2013.
,PubMed ID 24374807
[WNT1]
|
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Characterization of the Interaction of Sclerostin with the Low Density Lipoprotein Receptor-related Protein (LRP) Family of Wnt Co-receptors
,Gill Holdsworth, Patrick Slocombe, Carl Doyle, Bernadette Sweeney, Vaclav Veverka, Kelly Le Riche, Richard J. Franklin, Joanne Compson, Daniel Brookings, James Turner, Jeffery Kennedy, Rachael Garlish, Jiye Shi, Laura Newnham, David McMillan, Mariusz Muzylak, Mark D. Carr, Alistair J. Henry, Thomas Ceska, and Martyn K. Robinson,
J. Biol. Chem., Aug 2012; 287: 26464 - 26477.
[WNT1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210394 | WNT1 (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 1 (WNT1) |
CNY 3,656.00 |
|
| RC210394L1 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 1 (WNT1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210394L2 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 1 (WNT1), mGFP tagged |
CNY 5,890.00 |
|
| RC210394L3 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 1 (WNT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210394L4 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 1 (WNT1), mGFP tagged |
CNY 6,056.00 |
|
| RG210394 | WNT1 (tGFP-tagged) - Human wingless-type MMTV integration site family, member 1 (WNT1) |
CNY 5,256.00 |
