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Cytokeratin 1 (KRT1) (NM_006121) Human Untagged Clone
CAT＃: SC303735
KRT1 (untagged)-Human keratin 1 (KRT1)
CNY 7,200.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006121 edited
ATGAGTCGACAGTTTAGTTCCAGGTCTGGGTACCGAAGTGGAGGGGGCTTCAGCTCTGGC TCTGCTGGGATCATCAACTACCAGCGCAGGACCACCAGCAGCTCCACACGCCGCAGTGGA GGAGGTGGTGGGAGATTTTCAAGCTGTGGTGGTGGTGGTGGTAGCTTTGGTGCTGGTGGT GGATTTGGAAGTCGGAGTCTTGTTAACCTTGGTGGCAGTAAAAGCATCTCCATAAGTGTG GCTAGAGGAGGTGGACGTGGTAGTGGCTTTGGTGGTGGTTATGGTGGTGGTGGCTTTGGT GGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGAGGTGGCATTGGGGGTGGTGGCTTTGGT GGTTTTGGCAGTGGTGGTGGTGGTTTTGGTGGAGGTGGCTTTGGGGGTGGTGGATATGGG GGTGGTTATGGTCCTGTCTGCCCTCCTGGTGGCATACAAGAAGTCACTATCAACCAGAGC CTTCTTCAGCCCCTCAATGTGGAGATTGACCCTGAGATCCAAAAGGTGAAGTCTCGAGAA AGGGAGCAAATCAAGTCACTCAACAACCAATTTGCCTCCTTCATTGACAAGGTGAGGTTC CTGGAGCAGCAGAACCAGGTACTGCAAACAAAATGGGAGCTGCTGCAGCAGGTAGATACC TCCACTAGAACCCATAATTTAGAGCCCTACTTTGAGTCATTCATCAACAATCTCCGAAGG AGAGTGGACCAACTGAAGAGTGATCAATCTCGGTTGGATTCGGAACTGAAGAACATGCAG GACATGGTGGAGGATTACCGGAACAAGTATGAGGATGAAATCAACAAGCGGACAAATGCA GAGAATGAATTTGTGACCATCAAGAAGGATGTGGATGGTGCTTATATGACCAAGGTGGAC CTTCAGGCCAAACTTGACAACTTGCAGCAGGAAATTGATTTCCTTACAGCACTCTACCAA GCAGAGTTGTCTCAGATGCAGACTCAAATCAGTGAAACTAATGTCATCCTCTCTATGGAC AACAACCGCAGTCTCGACCTGGACAGCATCATTGCTGAGGTCAAGGCCCAGTACGAGGAT ATAGCCCAGAAGAGCAAAGCTGAGGCCGAGTCCTTGTACCAGAGCAAGTATGAAGAGCTG CAGATCACTGCTGGCAGACATGGGGATAGTGTGAGAAATTCAAAGATAGAAATTTCTGAG CTGAATCGTGTGATCCAGAGACTTAGATCTGAAATCGACAATGTCAAGAAGCAGATCTCC AACTTGCAGCAGTCCATCAGTGATGCAGAGCAGCGTGGCGAGAATGCCCTCAAGGATGCC AAGAACAAGCTGAATGACCTGGAGGATGCCCTGCAGCAGGCCAAGGAAGACCTGGCCCGC CTGCTGCGCGACTACCAGGAGCTGATGAACACCAAGCTGGCCCTGGATCTGGAGATTGCC ACCTACAGGACCCTCCTGGAGGGAGAAGAAAGCAGGATGTCTGGAGAATGTGCCCCGAAC GTGAGTGTGTCTGTGAGCACAAGCCACACCACCATCAGTGGAGGTGGCAGCCGAGGAGGT GGCGGCGGTGGCTACGGCTCTGGAGGTAGCAGCTATGGCTCCGGAGGTGGTAGCTATGGT TCTGGAGGTGGCGGCGGCGGCGGCCGTGGCAGCTATGGCTCCGGAGGTAGCAGCTACGGC TCCGGAGGTGGCAGCTATGGCTCTGGAGGTGGCGGCGGCGGCCATGGCAGCTACGGCTCC GGAAGCAGCAGTGGGGGCTACAGAGGTGGCTCTGGAGGCGGCGGCGGCGGCAGCTCTGGC GGCCGGGGCTCTGGCGGCGGGAGCTCTGGAGGCTCCATAGGAGGCCGGGGATCCAGCTCT GGGGGTGTCAAGTCCTCTGGTGGCAGTTCCAGCGTGAGGTTTGTTTCTACCACTTATTCC GGAGTAACCAGATAA >OriGene 5' read for NM_006121 unedited
CTTACTCTACCTTGCTCCTACTTTTCTCTAAGTCAACATGAGTCGACAGTTTAGTTCCAG GTCTGGGTACCGAAGTGGAGGGGGCTTCAGCTCTGGCTCTGCTGGGATCATCAACTACCA GCGCAGGACCACCAGCAGCTCCACACGCCGCAGTGGAGGAGGTGGTGGGAGATTTTCAAG CTGTGGTGGTGGTGGTGGTAGCTTTGGTGCTGGTGGTGGATTTGGAAGTCGGAGTCTTGT TAACCTTGGTGGCAGTAAAAGCATCTCCATAAGTGTGGCTAGAGGAGGTGGACGTGGTAG TGGCTTTGGTGGTGGTTATGGTGGTGGTGGCTTTGGTGGTGGTGGCTTTGGTGGTGGTGG CTTTGGTGGAGGTGGCATTGGGGGTGGTGGCTTTGGTGGTTTTGGCAGTGGTGGTGGTGG TTTTGGTGGAGGTGGCTTTGGGGGTGGTGGAT >OriGene 3' read for NM_006121 unedited
CATGGNGTGGCACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGCCACCC GGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGCGGCCGCCCTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAATTTCAAATGCAGTTTATTGCAGCAAA ACAAGGAAATGGGGAGTTTAAGACCTCTCCACAAAAAAAAAACAACTTGCTTACACCTTA TGTACAAAACCAAAACAGCACAGAGATAAGATGCTAAGGAGCTGTCCACACCCTGGGTCT AACTGGTCCTACTCTGGCTGGCTTAAGGAGGTGCTTTGAAATGTCATGTGGGTGGTGGTC ACTGCTGAACTGTTTCTCAGGGAAAGAACAAAGCAGGGTCATAGCCAGGGGACTGAGATT GCCACTGATCTGAAAACTTCATTGGGAAACAGCAGAAAAGAAAGAGCTGGGGGAATAGGA TGAGCTAGTGTAACTAACCATAGCTCTTTTCTCCGGTAAGGCTGGGACAAATCGACCTCG GTCTTGCCAAGCATATTATTTAGTGATGCTGGGGGAGAACTAGAGCTAATGAAACAGAGG GCATCTCTTTATCTGGTTACTCCGGAATAAGTGGTAGAAACAAACCTCACGCTGGAACTG CCACCAGAGGACTTGACACCCCCAGAGCTGGATCCCCCGCCTCCTATGGAGCCTCCAGAG CTCCCGCCGCCAGAGCCCCGGCCGCCAAAACTGCCGCCGCCCCCCGCCTTCAAAGCCACC TTTGTAGCCCCCATTGCTTGCTTCCGGAGCCCTAACTGTCATGGCCGCCGCCGCCCCCTT TCAGAACCCTAACTTGCCCCTTCCGGGAGCCGTAC |
| Restriction Sites | Please inquire |
| ACCN | NM_006121 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006121.2, NP_006112.2 |
| RefSeq Size | 2507 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 3848 |
| UniProt ID | P04264 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223146 | KRT1 (Myc-DDK-tagged)-Human keratin 1 (KRT1) |
CNY 7,192.00 |
|
| RC223146L1 | Lenti ORF clone of Human keratin 1 (KRT1), Myc-DDK-tagged |
CNY 9,592.00 |
|
| RC223146L2 | Lenti ORF clone of Human keratin 1 (KRT1), mGFP tagged |
CNY 9,592.00 |
|
| RC223146L3 | Lenti ORF clone of Human keratin 1 (KRT1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223146L4 | Lenti ORF clone of Human keratin 1 (KRT1), mGFP tagged |
CNY 6,460.00 |
|
| RG223146 | KRT1 (tGFP-tagged) - Human keratin 1 (KRT1) |
CNY 8,792.00 |
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