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EHMT2/G9A (EHMT2) (NM_006709) Human Untagged Clone
CAT＃: SC303814
EHMT2 (untagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a
CNY 23,370.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BAT8; C6orf30; G9A; GAT8; KMT1C; NG36 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_006709 edited
GCGCAAGCGGCGATGGCGGCGGCGGCGGGAGCTGCAGCGGCGGCGGCCGCCGAGGGGGAG GCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAAACCAGAGGAGCCACCGAGAGA GTTCATGGCTCTTTGGGGGACACCCCTCGTAGTGAAGAAACCCTGCCCAAGGCCACCCCC GACTCCCTGGAGCCTGCTGGCCCCTCATCTCCAGCCTCTGTCACTGTCACTGTTGGTGAT GAGGGGGCTGACACCCCTGTAGGGGCTACACCACTCATTGGGGATGAATCTGAGAATCTT GAGGGAGATGGGGACCTCCGTGGGGGCCGGATCCTGCTGGGCCATGCCACAAAGTCATTC CCCTCTTCCCCCAGCAAGGGGGGTTCCTGTCCTAGCCGGGCCAAGATGTCAATGACAGGG GCGGGAAAATCACCTCCATCTGTCCAGAGTTTGGCTATGAGGCTACTGAGTATGCCAGGA GCCCAGGGAGCTGCAGCAGCAGGGTCTGAACCCCCTCCAGCCACCACGAGCCCAGAGGGA CAGCCCAAGGTCCACCGAGCCCGCAAAACCATGTCCAAACCAGGAAATGGACAGCCCCCG GTCCCTGAGAAGCGGCCCCCTGAAATACAGCATTTCCGCATGAGTGATGATGTCCACTCA CTGGGAAAGGTGACCTCAGATCTGGCCAAAAGGAGGAAGCTGAACTCAGGAGGTGGCCTG TCGGAGGAGTTAGGTTCTGCCCGGCGTTCAGGAGAAGTGACCCTGACGAAAGGGGACCCC GGGTCCCTGGAGGAGTGGGAGACGGTGGTGGGTGATGACTTCAGTCTCTACTATGATTCC TACTCTGTGGATGAGCGCGTGGACTCCGACAGCAAGTCTGAAGTTGAAGCTCTAACTGAA CAACTAAGTGAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAAGAGGAGGAG GAAGAGGAAGAAGAAGAGGAAGATGAGGAGTCAGGGAATCAGTCAGATAGGAGTGGTTCC AGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGAAAAGACAGCCCATGGGTGAAGCCGTCT CGGAAACGGCGCAAGCGGGAGCCTCCGCGGGCCAAGGAGCCACGAGGAGTGAATGGTGTG GGCTCCTCAGGCCCCAGTGAGTACATGGAGGTCCCTCTGGGGTCCCTGGAGCTGCCCAGC GAGGGGACCCTCTCCCCCAACCACGCTGGGGTGTCCAATGACACATCTTCGCTGGAGACA GAGCGAGGGTTTGAGGAGTTGCCCCTGTGCAGCTGCCGCATGGAGGCACCCAAGATTGAC CGCATCAGCGAGAGGGCGGGGCACAAGTGCATGGCCACTGAGAGTGTGGACGGAGAGCTG TCAGGCTGCAATGCCGCCATCCTCAAGCGGGAGACCATGAGGCCATCCAGCCGTGTGGCC CTGATGGTGCTCTGTGAGACCCACCGCGCCCGCATGGTCAAACACCACTGCTGCCCGGGC TGCGGCTACTTCTGCACGGCGGGCACCTTCCTGGAGTGCCACCCTGACTTCCGTGTGGCC CACCGCTTCCACAAGGCCTGTGTGTCTCAGCTGAATGGGATGGTCTTCTGTCCCCACTGT GGGGAGGATGCTTCTGAAGCTCAAGAGGTGACCATCCCCCGGGGTGACGGGGTGACCCCA CCGGCCGGCACTGCAGCTCCTGCACCCCCACCCCTGTCCCAGGATGTCCCCGGGAGAGCA GACACTTCTCAGCCCAGTGCCCGGATGCGAGGGCATGGGGAACCCCGGCGCCCGCCCTGC GATCCCCTGGCTGACACCATTGACAGCTCAGGGCCCTCCCTGACCCTGCCCAATGGGGGC TGCCTTTCAGCCGTGGGGCTGCCACTGGGGCCAGGCCGGGAGGCCCTGGAAAAGGCCCTG GTCATCCAGGAGTCAGAGAGGCGGAAGAAGCTCCGTTTCCACCCTCGGCAGTTGTACCTG TCCGTGAAGCAGGGCGAGCTGCAGAAGGTGATCCTGATGCTGTTGGACAACCTGGACCCC AACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCATGCAGCCGCCCAGAAGGGC TCCGTGGAGATCTGCCATGTGCTGCTGCAGGCTGGAGCCAACATAAATGCAGTGGACAAA CAGCAGCGGACGCCACTGATGGAGGCCGTGGTGAACAACCACCTGGAGGTAGCCCGTTAC ATGGTGCAGCGTGGTGGCTGTGTCTATAGCAAGGAGGAGGACGGTTCCACCTGCCTCCAC CACGCAGCCAAAATCGGGAACTTGGAGATGGTCAGCCTGCTGCTGAGCACAGGACAGGTG GACGTCAACGCCCAGGACAGTGGGGGGTGGACGCCCATCATCTGGGCTGCAGAGCACAAG CACATCGAGGTGATCCGCATGCTACTGACGCGGGGCGCCGACGTCACCCTCACTGACAAC GAGGAGAACATCTGCCTGCACTGGGCCTCCTTCACGGGCAGCGCCGCCATCGCCGAAGTC CTTCTGAATGCGCGCTGTGACCTCCATGCTGTCAACTACCATGGGGACACCCCCCTGCAC ATCGCAGCTCGGGAGAGCTACCATGACTGCGTGCTGTTATTCCTGTCACGTGGGGCCAAC CCTGAGCTGCGGAACAAAGAGGGGGACACAGCATGGGACCTGACTCCCGAGCGCTCCGAC GTGTGGTTTGCGCTTCAACTCAACCGCAAGCTCCGACTTGGGGTGGGAAATCGGGCCATC CGCACAGAGAAGATCATCTGCCGGGACGTGGCTCGGGGCTATGAGAACGTGCCCATTCCC TGTGTCAACGGTGTGGATGGGGAGCCCTGCCCTGAGGATTACAAGTACATCTCAGAGAAC TGCGAGACGTCCACCATGAACATCGATCGCAACATCACCCACCTGCAGCACTGCACGTGT GTGGACGACTGCTCTAGCTCCAACTGCCTGTGCGGCCAGCTCAGCATCCGGTGCTGGTAT GACAAGGATGGGCGATTGCTCCAGGAATTTAACAAGATTGAGCCTCCGCTGATTTTCGAG TGTAACCAGGCGTGCTCATGCTGGAGAAACTGCAAGAACCGGGTCGTACAGAGTGGCATC AAGGTGCGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGGGGGGTCCGCGCCCTGCAG ACCATCCCACAGGGGACCTTCATCTGCGAGTATGTCGGGGAGCTGATCTCTGATGCTGAG GCTGATGTGAGAGAGGATGATTCTTACCTCTTCGACTTAGACAACAAGGATGGAGAGGTG TACTGCATAGATGCCCGTTACTATGGCAACATCAGCCGCTTCATCAACCACCTGTGTGAC CCCAACATCATTCCCGTCCGGGTCTTCATGCTGCACCAAGACCTGCGATTTCCACGCATC GCCTTCTTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTAGGGTTTGACTATGGCGAC CGCTTCTGGGACATCAAAAGCAAATATTTCACCTGCCAATGTGGCTCTGAGAAGTGCAAG CACTCAGCCGAAGCCATTGCCCTGGAGCAGAGCCGTCTGGCCCGCCTGGACCCACACCCT GAGCTGCTGCCCGAGCTCGGCTCCCTGCCCCCTGTCAACACATGAGAACGGACCACACCC TCTCTCCCCAGCATGGATGGCCACAGCTCAGCCGCCTCCTCTGCCACCAGCTGCTCGCAG CCCATGCCTGGGGGTGCTGCCATCTTCTCTCCCCACCACCCTTTCACACATTCCTGACCA GAGATCCCAGCCAGGCCCTGGAGGTCTGACAGCCCCTCCCTCCCAGAGCTGGTTCCTCCC TGGGAGGGCAACTTCAGGGCTGGCCACCCCCCGTGTTCCCCATCCTCAGTTGAAGTTTGA TGAATTGAAGTCGGGCCTCTATGCCAACTGGTTCCTTTTGTTCTCAATAAATGTTGGGTT TGGTAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_006709 unedited
ACCCGCCCCGTCTGAGCAACTGGGCGGTAGGCGCTGCACTGTGTTGGGAGGTTCTATTAT AAGCAGAGCTCATTTAGGTTGACACTATAGAATACAAGCTACTCTGTTCTTTTTGCAGCG GCCGCGAATTTCGGCACGAGGGCGCAAGCGGCGATGGCGGCGGCGGCGGGAGCCGCAGCG GCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAA ACCAGAGGAGCCACCGAGAGAGTTCATGGCTCTTTGGGGGACACCCCTCGTAGTGAAGAA ACCCTGCCCAAGGCCACCCCCGACTCCCTGGAGCCTGCTGGCCCCTCATCTCCAGCCTCT GTCACTGTCACTGTTGGTGATGAGGGGGCTGACACCCCTGTAGGGGCTACACCACTCATT GGGGATGAATCTGAGAATCTTGAGGGAGATGGGGACCTCCGTGGGGGCCGGATCCTGCTG GGCCATGCCACAAAGTCATTCCCCTCTTCCCCCAGCAAGGGGGGTTCCTGTCCTAGCCGG GCCAAGATGTCAATGACAGGGGCGGGAAAATCACCTCCATCTGTCCAGAGTTTGGCTATG AGGCTACTGAGTATGCCAGGAGCCCAGGGAGCTGCAGCAGCAGGGTCTGAACCCCCTCCA GCCACCACGAGCCCAGAGGGACAGCCCAAGGTCCACCGAGCCCGCAAAACCATGTCCAAA CCAGGAAATGGACAGCCCCCGGTCCCTGAGAAGCGGCCCCCTGAAATACAGCATTTCCGC ATGAGTGATGATGTCCACTCACTGGGAAAGGTGACCTCAGATCTGGCCAAAAGAGAAGCT GAACTCAGAGTGGCCTGTCGAGAGTAGTCTGCCCGCGTTCAGAGAGTGACCCTGACAAAG GACCCGGTCCTGAGATGGAGACGTGTGGGGTGATGACTCAGTCTCTACTATGATCCTACT CTGTGGATGAGCCCGTGGACTCGACG >Forward primer walk for NM_006709 unedited
TATTGCAGATGTTGTTGGAGACCTGCTCTAGCTCCACTAGCCATGTTGCGGCCAGCTTCA GCATCCGGTGCTGGTATTGACAAGGATGGGCGATTGCTCCAGGAATTTAACAAGATTGAG CCTCCGCTGATTTTCGAGCGTAACCAGGCGCGCTCATGCTGGAGAAACTGCAAGAACCGG GTCGTACAGAGTGGCATCAAGGTGCGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGG GGGGTCCGCGCCCTGCAGACCATCCCACAGGGGACCTTCATCTGCGAGTATGTCGGGGAG CTGACCTCTGATGCTGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGACTTAGAC AACAAGGATGGAGAGGTGTACTGCATAGATGCCCGTTACTATGGCAACATCAGCCGCTTC ATCAACCACCTGTGTGACCCCAACATCATTCCCGTCCGGGTCTTCATGCTGCACCAAGAC CTGCGATTTCCACGCATCGCCTTCTTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTA GGGTTTGACTATGGCGACCGCTTCTGGGACATCAAAAGCAAATATTTCACCTGCCAATGT GGCTCTGAGAAGTGCAAGCACTCAGCCGAAGCCATTGCCCTGGAGCAGAGCCGTCTGGCC CGCCTGGACCCACACCCTGAGCTGCTGCCCGAGCTCGGCTCCCTGCCCCCTGTCAACACA TGAGAACGGACCACACCCTCTCTCCCCAGCATGGATGGCCACAGCTCAGCCGCCTCCTCT GCCACCAGCTGCTCGCAGCCCATGCCTGGGGGTGCTGCCATCTCTCTCCCCACCACCCTT TCACACATTCCTGACCAGAGATCCCAGCCAGGCCCTGAGTCTGACAGCCCCTCCCTCCCA GAGCTGTTCCTCCCTGGGAGGGCAACTTC |
| Restriction Sites | Please inquire |
| ACCN | NM_006709 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006709.2, NP_006700.2 |
| RefSeq Size | 3994 bp |
| RefSeq ORF | 3633 bp |
| Locus ID | 10919 |
| UniProt ID | Q96KQ7 |
| Domains | SET, ANK, PreSET, Pre-SET |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| Gene Summary | This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (2, also known as NG36/G9a) uses an alternate 5' UTR and 5' coding region, contains an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon compared to variant 3. It encodes isoform a, which is shorter and has a distinct N-terminus, compared to isoform c. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224625 | EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a |
CNY 8,424.00 |
|
| RC224625L1 | Lenti-ORF clone of EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a |
CNY 10,824.00 |
|
| RC224625L2 | Lenti-ORF clone of EHMT2 (mGFP-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a |
CNY 14,630.00 |
|
| RC224625L3 | Lenti-ORF clone of EHMT2 (Myc-DDK-tagged)-Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a |
CNY 14,630.00 |
|
| RG224625 | EHMT2 (tGFP-tagged) - Human euchromatic histone-lysine N-methyltransferase 2 (EHMT2), transcript variant NG36/G9a |
CNY 10,024.00 |
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