Collagen V (COL5A3) (NM_015719) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304350 representing NM_015719. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAACCGCCGGGACCTGGGCCAGCCGCGGGCCGGTCTCTGCCTGCTCCTGGCCGCGCTGCAGCTT CTGCCGGGGACGCAGGCCGATCCTGTGGATGTCCTGAAGGCCCTGGGTGTGCAGGGAGGCCAGGCTGGG GTCCCCGAGGGGCCTGGCTTCTGTCCCCAGAGGACTCCAGAGGGTGACCGGGCATTCAGAATTGGCCAG GCCAGCACGCTCGGCATCCCCACGTGGGAACTCTTTCCAGAAGGCCACTTTCCTGAGAACTTCTCCTTG CTGATCACCTTGCGGGGACAGCCAGCCAATCAGTCTGTCCTGCTGTCCATTTATGATGAAAGGGGTGCC CGGCAGTTGGGCCTGGCACTGGGGCCAGCGCTGGGTCTCCTAGGTGACCCCTTCCGCCCCCTCCCCCAG CAGGTCAACCTCACAGATGGCAGGTGGCACCGTGTGGCCGTCAGCATAGATGGTGAGATGGTGACCCTG GTAGCTGACTGTGAAGCTCAGCCCCCTGTTTTGGGCCATGGCCCCCGCTTCATCAGCATAGCTGGACTC ACTGTGCTGGGGACCCAGGACCTTGGGGAAAAGACTTTCGAGGGAGACATTCAGGAGCTGCTGATAAGC CCAGATCCTCAGGCTGCCTTCCAGGCTTGTGAGCGGTACCTCCCCGACTGTGACAACCTGGCACCGGCA GCCACAGTGGCTCCCCAGGGTGAACCAGAAACCCCTCGTCCTCGGCGGAAGGGGAAGGGAAAAGGGAGG AAGAAAGGGCGAGGTCGCAAGGGGAAGGGCAGGAAAAAGAACAAGGAAATTTGGACCTCAAGTCCACCT CCTGACTCCGCAGAGAACCAGACCTCCACTGACATCCCCAAGACAGAGACTCCAGCTCCAAATCTGCCT CCGACCCCCACGCCTTTGGTCGTCACCTCCACTGTGACTACTGGACTCAATGCCACGATCCTAGAGAGG AGCTTGGACCCTGACAGTGGAACCGAGCTGGGGACCCTGGAGACCAAGGCAGCCAGGGAGGATGAAGAA GGAGATGATTCCACCATGGGCCCTGACTTCCGGGCAGCAGAATATCCATCTCGGACTCAGTTCCAGATC TTTCCTGGTGCTGGAGAGAAAGGAGCAAAAGGAGAGCCCGCAGTGATTGAAAAGGGGCAGCAGTTTGAG GGACCTCCAGGAGCCCCAGGACCCCAAGGGGTGGTTGGCCCCTCAGGCCCTCCCGGCCCCCCAGGATTC CCTGGCGACCCTGGTCCACCGGGCCCTGCTGGCCTCCCAGGAATCCCCGGCATTGATGGGATCCGAGGC CCACCGGGCACTGTGATCATGATGCCGTTCCAGTTTGCAGGCGGCTCCTTTAAAGGCCCCCCAGTCTCA TTCCAGCAGGCCCAGGCTCAGGCAGTTCTGCAGCAGACTCAGCTCTCTATGAAAGGCCCCCCTGGTCCA GTGGGGCTCACTGGGCGCCCAGGCCCTGTGGGTCTCCCCGGGCATCCAGGTCTGAAAGGAGAGGAGGGA GCAGAAGGGCCACAGGGTCCCCGAGGCCTGCAGGGACCTCATGGACCCCCTGGCCGAGTGGGCAAGATG GGCCGCCCTGGAGCAGATGGAGCTCGGGGCCTCCCAGGGGACACTGGACCTAAGGGTGATCGTGGCTTC GATGGCCTCCCTGGGCTGCCTGGTGAGAAGGGCCAAAGGGGTGACTTTGGCCATGTGGGGCAACCCGGT CCCCCAGGAGAGGATGGTGAGAGGGGAGCAGAGGGACCTCCAGGGCCCACTGGCCAGGCTGGGGAGCCG GGTCCACGAGGACTGCTTGGCCCCAGAGGCTCTCCTGGCCCCACGGGTCGCCCGGGTGTGACTGGAATT GATGGTGCTCCTGGTGCCAAAGGCAATGTGGGTCCTCCAGGAGAACCAGGCCCTCCGGGACAGCAGGGA AACCATGGGTCCCAGGGACTCCCCGGTCCCCAGGGACTCATTGGCACTCCTGGGGAGAAGGGTCCCCCT GGAAACCCAGGAATTCCAGGCCTCCCAGGATCCGATGGCCCTCTGGGTCACCCAGGACATGAGGGCCCC ACGGGAGAGAAAGGGGCTCAGGGTCCACCAGGGTCGGCAGGCCCTCCGGGCTATCCTGGACCTCGGGGA GTGAAGGGCACTTCAGGCAACCGGGGCCTCCAGGGGGAGAAAGGCGAGAAGGGAGAGGACGGCTTCCCA GGCTTCAAGGGCGATGTGGGGCTCAAAGGTGATCAGGGGAAACCCGGAGCTCCAGGTCCCCGGGGAGAG GATGGTCCTGAGGGGCCGAAGGGGCAGGCGGGGCAGGCTGGCGAGGAGGGGCCCCCAGGCTCAGCTGGG GAGAAGGGCAAGCTTGGGGTGCCAGGCCTCCCAGGTTATCCAGGACGCCCTGGACCTAAGGGATCTATT GGATTTCCCGGTCCCCTGGGACCCATAGGAGAGAAAGGGAAGTCGGGAAAGACAGGGCAGCCAGGCCTG GAAGGAGAGCGGGGACCACCAGGTTCCCGTGGAGAGAGGGGGCAACCGGGTGCCACAGGGCAACCAGGC CCCAAGGGCGATGTGGGCCAGGATGGAGCCCCTGGGATCCCTGGAGAAAAGGGCCTCCCTGGTCTGCAA GGCCCTCCAGGATTCCCTGGGCCAAAGGGCCCCCCTGGTCACCAAGGTAAAGATGGGCGACCAGGGCAC CCTGGACAGAGAGGAGAACTGGGCTTCCAAGGTCAGACAGGCCCGCCTGGACCAGCTGGTGTCTTAGGC CCTCAGGGAAAGACAGGAGAAGTGGGACCTCTAGGTGAAAGGGGGCCTCCAGGCCCCCCTGGACCTCCT GGTGAACAAGGTCTTCCTGGCCTGGAAGGCAGAGAGGGGGCCAAGGGGGAACTGGGACCACCAGGACCC CTTGGGAAAGAAGGGCCAGCTGGACTCAGGGGCTTTCCCGGCCCCAAAGGGGGCCCTGGGGACCCGGGA CCTACTGGCTTAAAGGGTGATAAGGGCCCCCCAGGGCCCGTGGGGGCCAATGGCTCCCCTGGTGAGCGC GGTCCTTTGGGCCCAGCAGGAGGCATTGGACTTCCTGGCCAAAGTGGCAGCGAAGGCCCCGTTGGCCCT GCAGGCAAGAAGGGGTCCCGGGGAGAACGTGGCCCCCCTGGCCCCACTGGCAAAGATGGGATCCCAGGG CCCCTGGGGCCTCTGGGACCCCCTGGAGCTGCTGGGCCTTCTGGCGAGGAAGGGGACAAGGGGGATGTG GGTGCCCCCGGACACAAGGGGAGTAAAGGCGATAAAGGAGACGCGGGCCCACCTGGACAACCAGGGATA CGGGGTCCTGCAGGACACCCAGGTCCCCCGGGAGCAGACGGGGCTCAGGGGCGCCGGGGACCCCCAGGC CTCTTTGGGCAGAAAGGAGATGACGGAGTCAGAGGCTTTGTGGGGGTGATTGGCCCTCCTGGACTGCAG GGGCTGCCAGGCCCTCCGGGAGAGAAAGGGGAGGTCGGAGACGTCGGGTCCATGGGTCCCCATGGAGCT CCAGGTCCTCGGGGTCCCCAAGGCCCCACTGGATCAGAGGGCACTCCAGGGCTGCCTGGAGGAGTTGGT CAGCCAGGCGCCGTGGGTGAGAAGGGTGAGCGAGGGGACGCTGGAGACCCAGGGCCTCCAGGAGCCCCA GGCATCCCGGGGCCCAAGGGAGACATTGGTGAAAAGGGGGACTCAGGCCCATCTGGAGCTGCTGGACCC CCAGGCAAGAAAGGTCCCCCTGGAGAGGATGGAGCCAAAGGGAGCGTGGGCCCCACGGGGCTGCCCGGA GATCTAGGGCCCCCAGGAGACCCTGGAGTTTCAGGCATAGATGGTTCCCCAGGGGAGAAGGGAGACCCT GGTGATGTTGGGGGACCGGGTCCGCCTGGAGCTTCTGGGGAGCCCGGCGCCCCCGGGCCCCCCGGCAAG AGGGGTCCTTCAGGCCACATGGGTCGAGAAGGCAGAGAAGGGGAGAAAGGTGCCAAGGGGGAGCCAGGT CCTGATGGGCCCCCAGGGAGGACGGGTCCAATGGGGGCTAGAGGGCCCCCTGGACGTGTGGGGCCTGAG GGTCTTCGAGGGATCCCTGGCCCTGTGGGTGAACCAGGCCTCCTGGGAGCCCCTGGACAGATGGGCCCT CCTGGCCCCCTGGGGCCCTCTGGCCTCCCAGGGCTGAAGGGAGACACTGGCCCCAAGGGGGAAAAGGGC CACATTGGATTGATCGGTCTCATTGGCCCCCCGGGAGAAGCTGGTGAGAAAGGAGATCAGGGGTTGCCA GGCGTGCAGGGACCCCCTGGTCCCAAGGGAGACCCTGGTCCCCCTGGTCCCATTGGCTCTCTGGGCCAC CCTGGGCCCCCAGGTGTGGCGGGCCCTCTAGGACAGAAAGGCTCAAAAGGGTCTCCGGGGTCCATGGGC CCCCGTGGAGACACTGGACCTGCAGGCCCACCAGGCCCCCCGGGTGCCCCTGCCGAGCTGCATGGGCTG CGCAGGCGCCGGCGCTTCGTCCCAGTCCCGCTTCCAGTCGTGGAGGGCGGCCTGGAGGAGGTGCTGGCC TCGCTCACATCGCTGAGCTTGGAGCTGGAGCAGCTGCGGCGTCCTCCCGGCACTGCGGAGCGCCCGGGC CTCGTGTGCCACGAGCTGCACCGCAACCACCCGCACCTGCCTGATGGGGAATACTGGATTGACCCCAAC CAGGGCTGCGCGCGGGACTCGTTCAGGGTTTTTTGCAACTTCACGGCGGGAGGAGAGACCTGCCTCTAT CCCGACAAGAAGTTTGAGATCGTGAAATTGGCCTCCTGGTCCAAGGAAAAGCCTGGAGGCTGGTATAGC ACATTCCGTCGAGGGAAGAAGTTCTCCTACGTGGACGCCGACGGGTCCCCAGTGAATGTCGTGCAGCTG AACTTCCTGAAACTGCTGAGTGCCACAGCTCGCCAGAACTTCACCTACTCCTGCCAGAATGCAGCTGCC TGGCTGGACGAAGCCACGGGTGACTACAGCCACTCCGCCCGCTTCCTTGGCACCAATGGAGAGGAGCTG TCTTTCAACCAGACGACAGCAGCCACTGTCAGCGTCCCCCAGGATGGCTGCCGGCTCCGGAAAGGACAG ACGAAGACCCTTTTCGAATTCAGCTCTTCTCGAGCGGGATTTCTGCCCCTGTGGGATGTGGCGGCCACT GACTTTGGCCAGACGAACCAAAAGTTTGGGTTTGAACTGGGCCCCGTCTGCTTCAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015719
Insert Size	5238 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015719.3
RefSeq Size	6192 bp
RefSeq ORF	5238 bp
Locus ID	50509
UniProt ID	P25940
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	172.1 kDa
Gene Summary	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]

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