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KCC2 (SLC12A5) (NM_020708) Human Untagged Clone
CAT＃: SC304801
SLC12A5 (untagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2
CNY 7,776.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE34; EIEE34; EIG14; hKCC2; KCC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_020708 edited
GGGCGGCCGCGAATTCGCCCTTAGAGCTACAGCGAACGAGAGAGCGGCGAAGGCGGGTAG AGGGGCGCGGGCGAGGCGGCGCAGCCATCCCCGGACCAGGGGCCGCGCCGCCACCATGCT AAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCGGGTGATGGCAACCC CAAGGAAAGCAGTCCCTTCATCAACAGCACCGACACAGAGAAGGGAAAGGAGTATGATGG CAAGAACATGGCCTTGTTTGAGGAGGAGATGGACACCAGCCCTATGGTGTCCTCCTTGCT CAGTGGCCTGGCCAACTACACCAACCTGCCCCAGGGAAGTAGGGAGCATGAAGAGGCAGA AAACAATGAGGGTGGAAAAAAGAAGCCGGTGCAGGCCCCACGCATGGGCACCTTCATGGG CGTGTACCTGCCGTGCCTGCAGAACATCTTTGGCGTCATCCTCTTCCTGCGGCTCACCTG GGTGGTGGGCATTGCAGGCATCATGGAGTCCTTCTGCATGGTGTTCATCTGCTGCTCCTG TACGATGCTCACGGCCATCTCCATGAGTGCAATTGCAACGAATGGTGTTGTGCCTGCTGG TGGCTCCTACTACATGATTTCCAGGTCTCTGGGCCCAGAGTTTGGGGGTGCCGTGGGCCT CTGCTTCTACCTGGGCACTACCTTTGCAGGAGCCATGTACATCCTGGGCACCATCGAAAT CCTGCTGGCTTACCTCTTCCCAGCCATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGA GGCAGCAGCCATGCTGAACAACATGCGTGTTTACGGCACCTGTGTGCTCACCTGCATGGC CACTGTGGTGTTTGTGGGTGTCAAGTATGTCAACAAGTTTGCCCTTGTCTTCCTGGGTTG TGTCATCCTCTCCATCCTGGCCATCTATGCTGGGGTCATCAAGTCTGCCTTCGACCCACC CAACTTCCCGATCTGCCTCCTGGGTAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTG TGCCAAGCTGGCTTGGGAAGGAAATGAGACGGTGACCACACGGCTATGGGGCCTTTTCTG CTCCTCTCGCTTCCTCAACGCCACCTGTGATGAATACTTCACCCGAAACAATGTCACAGA GATCCAGGGCATCCCTGGTGCTGCCAGTGGCCTCATCAAAGAGAACCTCTGGAGCTCCTA CCTGACCAAGGGCGTGATTGTGGAGAGGAGTGGGATGACCTCGGTGGGCCTGGCCGATGG CACTCCTATCGACATGGACCACCCTTATGTCTTCAGTGATATGACCTCCTACTTCACCCT GCTGGTTGGCATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGTTCTAACCGCTCTGG GGACCTGAGGGATGCCCAGAAGTCAATCCCCACTGGCACCATCCTGGCCATCGCCACCAC CTCTGCTGTCTACATCAGCTCCGTTGTTCTGTTTGGGGCCTGCATTGAGGGGGTCGTCCT GCGGGACAAGTTTGGCGAAGCTGTGAATGGCAACCTCGTGGTGGGCACTCTGGCCTGGCC ATCTCCATGGGTAATTGTCATCGGATCCTTCTTCTCCACCTGTGGGGCTGGGCTGCAGAG CCTCACGGGGGCCCCACGCCTGCTGCAGGCCATCTCGAGGGATGGCATTGTGCCCTTCCT GCAGGTCTTTGGCCATGGCAAGGCCAATGGAGAGCCGACCTGGGCCCTGCTCCTGACTGC CTGCATCTGCGAGATTGGCATCCTCATTGCATCCCTCGACGAGGTGGCCCCCATCCTCTC TATGTTCTTCCTGATGTGCTACATGTTTGTGAATCTGGCCTGTGCAGTGCAGACGCTGCT GAGGACACCCAACTGGAGGCCACGCTTTCGATATTACCACTGGACCCTCTCCTTCCTGGG CATGAGCCTCTGCCTGGCCCTCATGTTCATCTGCTCCTGGTATTATGCACTGGTAGCCAT GCTCATTGCTGGACTCATCTACAAGTACATTGAGTACCGTGGGGCAGAGAAGGAGTGGGG CGATGGGATACGAGGTCTGTCTCTCAGTGCGGCTCGCTATGCCCTCTTACGCCTGGAGGA AGGGCCCCCACACACCAAGAACTGGAGGCCACAGCTGCTGGTGCTGGTGCGTGTGGACCA AGACCAGAATGTGGTGCACCCCCAGCTGCTCTCACTGACCTCCCAGCTGAAGGCAGGGAA GGGCCTGACCATCGTGGGCTCTGTCCTTGAGGGCACCTTTCTGGAAAATCATCCACAGGC CCAGCGGGCAGAAGAGTCTATCAGGCGCCTGATGGAGGCAGAGAAGGTGAAGGGCTTCTG CCAGGTGGTGATCTCCTCCAACTTGCGTGATGGCGTGTCCCATCTGATCCAGTCCGGGGG CCTCGGGGGGCTGCAGCACAACACTGTGCTTGTTGGCTGGCCCCGCAACTGGCGCCAGAA GGAAGATCATCAGACATGGAGGAACTTCATTGAGCTGGTCCGGGAAACCACAGCTGGCCA CTTAGCCCTGCTGGTCACCAAGAACGTTTCCATGTTTCCTGGGAACCCTGAGCGCTTCTC TGAGGGCAGCATCGACGTTTGGTGGATTGTGCACGATGGAGGCATGCTCATGCTGCTGCC CTTCCTGCTGCGGCACCACAAGGTCTGGCGGAAGTGCAAGATGCGTATCTTCACTGTGGC CCAGATGGATGACAATAGCATCCAGATGAAGAAGGATCTGACCACATTTCTGTATCATTT ACGCATCACTGCGGAGGTCGAGGTGGTGGAGATGCATGAGAGCGACATCTCAGCTTACAC CTATGAGAAGACGTTGGTGATGGAGCAGCGTTCCCAGATCCTCAAACAGATGCATTTAAC CAAGAATGAGCGGGAGCGGGAGATCCAGAGTATCACAGATGAGTCACGAGGCTCAATCCG GAGAAAGAATCCAGCCAACACGCGGCTCCGCCTGAACGTCCCAGAAGAGACGGCTGGTGA CAGTGAAGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCACGATCAGAGTGCTCCCAGCTG CCCCAGCAGCTCCCCGTCCCCAGGGGAGGAGCCTGAGGGGGAAGGGGAGACAGATCCGGA GAAGGTGCATCTCACCTGGACCAAGGACAAGTCGGTGGCAGAGAAGAATAAGGGCCCCAG TCCTGTCTCCTCTGAGGGCATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAGAACTT GAACCAGTCCAACGTGCGGCGCATGCACACGGCCGTGCGGCTGAACGAGGTCATCGTGAA GAAATCCCGGGACGCCAAGCTTGTTTTGCTCAACATGCCTGGGCCTCCCCGCAACCGCAA TGGTGATGAAAACTACATGGAGTTTCTCGAGGTCCTCACAGAGCACCTGGACCGGGTGAT GCTGGTCCGCGGCGGCGGCCGCGAGGTCATCACCATCTACTCCTGAGAACCAGGACCTGC CACCCGGGCCCGAGCGCGCCCGGCCCGCGGCTCCGGAGCCCTCGCCGCGCCCCCCGCCGC TGTCACCGTTTACATACAGACCCTGTGCCCGTGTCCTGGCCCCTTACCCCGCTGCCTGAA GCCCGGAGGCCACGCCTGTTGGGGCTGATTCGGAGAGGGCGCCCCGCCGCGCAGAGACCA GAGCTCCTCAGTGCCAGTTTGGCCCCTGGGTCTTCGCTGCCCTTTTTCTAAGCCCGGCCT CGTCTCGCCGGAGGAGACGCTGCAATAAAGGTTGGGAGAAGGCGCGGAAAGGAGAGGAGC TGGGGCCTTGGGGACCCCCAGGTAGTCCATGCGGCCCATTCCTCCCCTTCCCACTCCCGC CGCGGTCCTCGCTCTGCGCTCCTCCGGCGCTGCTCCCTGGCTCCCGGCGGCCCGGAGGCC CGCGGGGTGGGAAGGCCGCGCTTGCCGTCTCCGCCGCCCCTTCTCGCCGAGCCGTGGGGC GCGGGCGGCCGAGCCTATACATAGTGTACAGGAGACATCGCGTGTATTTTTAACGTCCCC ATATTTATGTGACTAGAAGCGCAACAGACTTCTCG >OriGene 5' read for NM_020708 unedited
NGGCGTCAAAATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGCCCTTAGAGCTAC AGCGAACGAGAGAGCGGCGAAGGCGGGTAGAGGGGCGCGGGCGAGGCGGCGCAGCCATCC CCGGACAGGGGCCGCGCCGCCACCATGCTAAACAACCTGACGGACTGCGAGGACGGCGAT GGGGGAGCCAACCCGGGTGATGGCAACCCCAAGGAAAGCAGTCCCTTCATCAACAGCACC GACACAGAGAAGGGAAAGGAGTATGATGGCAAGAACATGGCCTTGTTTGAGGAGGAGATG GACACCAGCCCTATGGTGTCCTCCTTGCTCAGTGGCCTGGCCAACTACACCAACCTGCCC CAGGGAAGTAGGGAGCATGAAGAGGCAGAAAACAATGAGGGTGGAAAAAAGAAGCCGGTG CAGGCCCCACGCATGGGCACCTTCATGGGCGTGTACCTGCCGTGCCTGCAGAACATCTTT GGCGTCATCCTCTTCCTGCGGCTCACCTGGGTGGTGGGCATTGCAGGCATCATGGAGTCC TTCTGCATGGTGTTCATCTGCTGCTCCTGTACGATGCTCACGGCCATCTCCATGAGTGCA ATTGCAACGAATGGTGTTGTGCCTGCTGGTGGCTCCTACTACATGATTTCCAGGTCTCTG GGCCCAGAGTTTGGGGGTGCCGTGNGCCTCTGCTTCTACCTGGGCACTACCTTTGCAGGA GCCATGTACATCCTGGGCACCATCGAAATCCTGCTGGCTTACCTCTTCCCAGCCATGGCC ATCTTCAAGGCAAAGATGCCAGTGGGGAGGCCACACCCATGCTGAACACATGCGTGTTTA CGGCACCTGTGTGCTCACCTGCATGGCCACTGGGGG >OriGene 3' read for NM_020708 unedited
NCATTGGNGATGGCACTTCCAGGNCCAGNATAGCACTGGGGNAGGGTCACAGGGNAGCCA CCCGGGATCTGTTCAGGAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT TTTTTTTTTTTAAGGGTATAATAAAATTATTTTTATTGCATTTTGTGCAGACGGGAGTCT AAAAAAGTAATACAGTAAAAGCCATAAAGCATTAAATGGGAGCATTCAACAATAAAGGAA AGAACCAACCCTCTCCTTTTCATATTGGCACAAAGAACACAAGATCAATACTGAGGCAGG ATGCGGCATACTGAACACTTACACTGAAAATTAAAGGCAGGCCTAATAATAATAGCAGTA ATAGTAATAAATACTCTTGATTTGGTTTGCAACCTCTCCTATTTACAAGGGTTTTCAACT TCATTGCATTGCCCTGCACATAGATCATCTAAAACAAAAGCAACACTAGTTATCACTACA AGGCTATTGAAATATTGCACTCAGAATAAAAGTCACTTGAATGGACATGAAACATCTAAC TACAGTGATTATAAAGATAACTTGATGAATGACTTGTGATAACATTCATAACAAATATTG CACATGTAGCACAGAAAATTTGGGTTTTTGTTGTGGGATGTCTGTTTCATCAACGGAAAC TTTGCTCTTGCCAACCAGACAATCTGGGCCCAGATAGTGCAACTTTGGGTCCCTAATTGA TGTTGATACAGCCCCCTTTCTGCACAGCCCATCACATCAGCTCTGGAATATTCAGGCCCC CANAAAGGCAGACCTGGCTGTGGTGTTCACAAGGAGGGCTACTGAAAGATGCAGGGGCCT GGTGTCTTAACACCTCCAGCTCCCTTGTTGAGTACTTGAAAGAATCTATCATGCCCC |
| Restriction Sites | Please inquire |
| ACCN | NM_020708 |
| Insert Size | 3351 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020708.3, NP_065759.1 |
| RefSeq Size | 6059 bp |
| RefSeq ORF | 3351 bp |
| Locus ID | 57468 |
| UniProt ID | Q9H2X9 |
| Protein Families | Transmembrane |
| Gene Summary | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2, also known as KCC2b) has a distinct N-terminus and is shorter than isoform 1. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation
,Conway, LC;Cardarelli, RA;Moore, YE;Jones, K;,
J Biol Chem.
,PubMed ID 29092909
[SLC12A5]
|
|
Mutations in SLC12A5 in epilepsy of infancy with migrating focal seizures
,Stödberg, T;McTague, A;Ruiz, AJ;Hirata, H;Zhen, J;Long, P;Farabella, I;Meyer, E;Kawahara, A;Vassallo, G;Stivaros, SM;Bjursell, MK;Stranneheim, H;Tigerschiöld, S;Persson, B;Bangash, I;Das, K;Hughes, D;Lesko, N;Lundeberg, J;Scott, RC;Poduri, A;Scheffer, IE;Smith, H;Gissen, P;Schorge, S;Reith, ME;Topf, M;Kullmann, DM;Harvey, RJ;Wedell, A;Kurian, MA;,
Nat Commun
,PubMed ID 26333769
[SLC12A5]
|
|
The potassium–chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism
,Wei-Chun Wei, Colin J. Akerman, Sarah E. Newey, Jiliu Pan, Nicholas W. V. Clinch, Yves Jacob, Meng-Ru Shen, Robert J. Wilkins, and J. Clive Ellory,
J. Physiol., Nov 2011; 589: 5349 - 5359.
[SLC12A5]
|
|
A Thallium Transport FLIPR-Based Assay for the Identification of KCC2-Positive Modulators
,Di Zhang, Sujatha M. Gopalakrishnan, Gail Freiberg, and Carol S. Surowy,
J Biomol Screen, Feb 2010; 15: 177 - 184
[SLC12A5]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223680 | SLC12A5 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2 |
CNY 7,768.00 |
|
| RC223680L1 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, Myc-DDK-tagged |
CNY 10,168.00 |
|
| RC223680L2 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, mGFP tagged |
CNY 10,260.00 |
|
| RC223680L3 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC223680L4 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, mGFP tagged |
CNY 10,260.00 |
|
| RG223680 | SLC12A5 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2 |
CNY 9,368.00 |
