Alanyl tRNA synthetase 2 (AARS2) (NM_020745) Human Untagged Clone
CAT#: SC304814
AARS2 (untagged)-Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein
CNY 18,910.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AARSL; COXPD8; LKENP; MT-ALARS; MTALARS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC304814 representing NM_020745.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGCGTCAGTGGCAGCTGCAGCCCGGAGGCTGCGGCGGGCCATTCGAAGGTCGCCCGCATGGCGG GGCCTCAGCCATCGGCCGCTCTCATCGGAGCCCCCTGCAGCCAAGGCCTCGGCCGTGAGGGCCGCCTTT CTGAACTTCTTTCGGGACCGCCATGGCCACCGGCTGGTGCCCTCCGCTTCCGTGCGGCCCCGCGGCGAC CCCAGTTTGCTTTTTGTCAATGCGGGCATGAACCAGTTCAAGCCAATCTTTCTGGGCACCGTGGATCCA CGAAGCGAGATGGCAGGCTTCCGACGTGTGGCCAACAGCCAGAAATGTGTGAGAGCTGGAGGACACCAT AACGACCTGGAAGATGTGGGTCGAGACCTTTCCCATCATACCTTCTTTGAAATGCTTGGCAATTGGGCC TTTGGGGGTGAATATTTTAAGGAGGAGGCTTGTAACATGGCCTGGGAACTGCTGACTCAGGTCTATGGG ATCCCTGAGGAAAGGCTCTGGATCTCCTACTTTGATGGTGACCCCAAGGCAGGGCTGGACCCAGACCTG GAGACCAGGGACATCTGGCTGAGCTTAGGGGTGCCTGCTAGCCGTGTGCTTTCCTTTGGACCACAAGAG AACTTCTGGGAGATGGGGGATACTGGCCCTTGTGGGCCCTGTACTGAGATCCACTACGACCTTGCTGGT GGGGTGGGAGCCCCCCAGCTGGTAGAGCTTTGGAACCTGGTCTTCATGCAACACAACAGAGAGGCAGAT GGAAGCCTGCAGCCCCTGCCCCAGCGGCATGTGGACACAGGAATGGGCCTGGAAAGGCTGGTGGCTGTG CTGCAAGGCAAACACTCCACCTATGACACTGACCTCTTTTCCCCGCTGCTCAACGCCATACAGCAGGGC TGCAGGGCACCCCCTTACTTGGGCCGAGTAGGGGTGGCAGACGAGGGGCGCACAGACACAGCGTACCGC GTGGTGGCTGACCACATCCGCACACTCAGTGTCTGCATCTCTGATGGCGTCTTCCCTGGGATGTCAGGT CCCCCGCTGGTTCTTCGTCGGATCCTGCGTCGAGCTGTGCGTTTCTCCATGGAGATCTTAAAGGCACCA CCTGGCTTCCTAGGCAGCCTGGTACCTGTAGTGGTGGAGACACTGGGAGATGCTTATCCAGAACTGCAA AGGAACTCAGCCCAGATCGCCAACCTGGTGTCAGAGGACGAGGCAGCCTTCCTGGCCTCCCTGGAGCGG GGTAGGCGGATCATTGATCGGACTCTGAGGACCCTGGGGCCTTCAGATATGTTCCCTGCTGAAGTGGCC TGGTCCTTGTCACTGTGTGGAGACCTGGGACTCCCCTTGGACATGGTAGAGCTGATGCTGGAGGAGAAA GGGGTCCAGCTAGACTCCGCTGGACTGGAGCGGTTGGCCCAAGAGGAGGCCCAGCACCGGGCACGGCAG GCTGAGCCAGTTCAGAAGCAGGGATTGTGGCTTGATGTCCATGCGCTTGGGGAGCTGCAGCGCCAAGGA GTGCCCCCAACTGACGACAGCCCCAAGTACAACTACTCCCTGCGACCCAGCGGAAGTTATGAGTTCGGC ACCTGTGAGGCCCAGGTGTTGCAACTGTATACAGAGGACGGGACAGCAGTGGCCTCCGTGGGGAAAGGC CAGCGCTGTGGCCTCCTCTTGGACAGGACCAACTTCTACGCAGAACAGGGGGGCCAGGCTTCAGACCGT GGCTACCTGGTGCGGGCAGGGCAAGAGGACGTGCTGTTCCCAGTAGCCCGGGCCCAGGTCTGTGGAGGT TTCATCCTGCATGAGGCAGTAGCCCCTGAGTGCCTGCGGTTAGGGGACCAGGTGCAGCTGCATGTGGAT GAGGCCTGGCGTCTAGGCTGCATGGCGAAGCATACGGCCACCCACCTGCTGAACTGGGCACTGAGGCAG ACCCTGGGCCCTGGCACAGAGCAGCAGGGCTCCCATCTCAATCCTGAGCAGCTGCGCTTGGATGTGACC ACCCAGACCCCATTGACCCCAGAGCAGCTCCGGGCAGTGGAGAACACTGTGCAGGAGGCCGTGGGGCAG GATGAGGCTGTGTACATGGAGGAGGTGCCCCTGGCGCTCACTGCCCAGGTCCCTGGCCTGCGCTCTCTG GATGAGGTTTACCCAGACCCTGTGCGGGTGGTATCAGTGGGGGTGCCCGTGGCCCATGCATTGGACCCA GCCTCCCAAGCCGCACTGCAGACCTCTGTGGAGCTATGCTGTGGGACGCACCTGTTACGTACTGGGGCT GTAGGGGACCTGGTTATCATCGGGGACCGCCAGCTTTCCAAGGGCACTACCCGCCTGCTGGCCGTCACT GGGGAGCAGGCCCAGCAGGCCCGAGAGCTAGGCCAGAGCCTGGCCCAGGAAGTGAAAGCGGCCACTGAG CGGCTGAGTCTGGGGAGCCGGGATGTGGCGGAGGCACTGAGGCTGTCCAAGGACATAGGACGACTCATT GAAGCTGTGGAAACTGCTGTGATGCCCCAGTGGCAGCGGCGGGAGCTGCTGGCCACAGTGAAGATGCTG CAGCGGCGTGCCAACACTGCCATCCGTAAGCTGCAAATGGGACAGGCTGCAAAGAAAACTCAGGAGCTG CTGGAGCGGCACTCGAAGGGGCCTCTGATTGTGGACACAGTCTCTGCTGAGTCTCTCTCAGTGCTGGTG AAGGTGGTACGGCAGCTGTGTGAGCAGGCCCCCAGCACGTCTGTGCTCCTACTCAGCCCCCAGCCCATG GGGAAGGTGCTGTGTGCCTGTCAGGTGGCCCAGGGTGCCATGCCCACCTTCACAGCAGAGGCCTGGGCA CTGGCAGTGTGCAGCCACATGGGGGGCAAGGCGTGGGGCTCACGAGTGGTGGCCCAAGGCACCGGAAGC ACTACTGACCTGGAAGCTGCCCTCAGTATAGCCCAAACCTATGCCCTCAGCCAGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_020745 |
Insert Size | 2958 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020745.3 |
RefSeq Size | 4782 bp |
RefSeq ORF | 2958 bp |
Locus ID | 57505 |
UniProt ID | Q5JTZ9 |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
MW | 107.3 kDa |
Gene Summary | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223715 | AARS2 (Myc-DDK-tagged)-Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein |
CNY 7,216.00 |
|
RC223715L3 | Lenti ORF clone of Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 9,030.00 |
|
RC223715L4 | Lenti ORF clone of Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 9,030.00 |
|
RG223715 | AARS2 (tGFP-tagged) - Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein |
CNY 7,890.00 |
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