Alanyl tRNA synthetase 2 (AARS2) (NM_020745) Human Untagged Clone

CAT#: SC304814

AARS2 (untagged)-Human alanyl-tRNA synthetase 2, mitochondrial (putative) (AARS2), nuclear gene encoding mitochondrial protein



  "NM_020745" in other vectors (4)

CNY 18,910.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-AARS2 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AARSL; COXPD8; LKENP; MT-ALARS; MTALARS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC304814 representing NM_020745.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCAGCGTCAGTGGCAGCTGCAGCCCGGAGGCTGCGGCGGGCCATTCGAAGGTCGCCCGCATGGCGG
GGCCTCAGCCATCGGCCGCTCTCATCGGAGCCCCCTGCAGCCAAGGCCTCGGCCGTGAGGGCCGCCTTT
CTGAACTTCTTTCGGGACCGCCATGGCCACCGGCTGGTGCCCTCCGCTTCCGTGCGGCCCCGCGGCGAC
CCCAGTTTGCTTTTTGTCAATGCGGGCATGAACCAGTTCAAGCCAATCTTTCTGGGCACCGTGGATCCA
CGAAGCGAGATGGCAGGCTTCCGACGTGTGGCCAACAGCCAGAAATGTGTGAGAGCTGGAGGACACCAT
AACGACCTGGAAGATGTGGGTCGAGACCTTTCCCATCATACCTTCTTTGAAATGCTTGGCAATTGGGCC
TTTGGGGGTGAATATTTTAAGGAGGAGGCTTGTAACATGGCCTGGGAACTGCTGACTCAGGTCTATGGG
ATCCCTGAGGAAAGGCTCTGGATCTCCTACTTTGATGGTGACCCCAAGGCAGGGCTGGACCCAGACCTG
GAGACCAGGGACATCTGGCTGAGCTTAGGGGTGCCTGCTAGCCGTGTGCTTTCCTTTGGACCACAAGAG
AACTTCTGGGAGATGGGGGATACTGGCCCTTGTGGGCCCTGTACTGAGATCCACTACGACCTTGCTGGT
GGGGTGGGAGCCCCCCAGCTGGTAGAGCTTTGGAACCTGGTCTTCATGCAACACAACAGAGAGGCAGAT
GGAAGCCTGCAGCCCCTGCCCCAGCGGCATGTGGACACAGGAATGGGCCTGGAAAGGCTGGTGGCTGTG
CTGCAAGGCAAACACTCCACCTATGACACTGACCTCTTTTCCCCGCTGCTCAACGCCATACAGCAGGGC
TGCAGGGCACCCCCTTACTTGGGCCGAGTAGGGGTGGCAGACGAGGGGCGCACAGACACAGCGTACCGC
GTGGTGGCTGACCACATCCGCACACTCAGTGTCTGCATCTCTGATGGCGTCTTCCCTGGGATGTCAGGT
CCCCCGCTGGTTCTTCGTCGGATCCTGCGTCGAGCTGTGCGTTTCTCCATGGAGATCTTAAAGGCACCA
CCTGGCTTCCTAGGCAGCCTGGTACCTGTAGTGGTGGAGACACTGGGAGATGCTTATCCAGAACTGCAA
AGGAACTCAGCCCAGATCGCCAACCTGGTGTCAGAGGACGAGGCAGCCTTCCTGGCCTCCCTGGAGCGG
GGTAGGCGGATCATTGATCGGACTCTGAGGACCCTGGGGCCTTCAGATATGTTCCCTGCTGAAGTGGCC
TGGTCCTTGTCACTGTGTGGAGACCTGGGACTCCCCTTGGACATGGTAGAGCTGATGCTGGAGGAGAAA
GGGGTCCAGCTAGACTCCGCTGGACTGGAGCGGTTGGCCCAAGAGGAGGCCCAGCACCGGGCACGGCAG
GCTGAGCCAGTTCAGAAGCAGGGATTGTGGCTTGATGTCCATGCGCTTGGGGAGCTGCAGCGCCAAGGA
GTGCCCCCAACTGACGACAGCCCCAAGTACAACTACTCCCTGCGACCCAGCGGAAGTTATGAGTTCGGC
ACCTGTGAGGCCCAGGTGTTGCAACTGTATACAGAGGACGGGACAGCAGTGGCCTCCGTGGGGAAAGGC
CAGCGCTGTGGCCTCCTCTTGGACAGGACCAACTTCTACGCAGAACAGGGGGGCCAGGCTTCAGACCGT
GGCTACCTGGTGCGGGCAGGGCAAGAGGACGTGCTGTTCCCAGTAGCCCGGGCCCAGGTCTGTGGAGGT
TTCATCCTGCATGAGGCAGTAGCCCCTGAGTGCCTGCGGTTAGGGGACCAGGTGCAGCTGCATGTGGAT
GAGGCCTGGCGTCTAGGCTGCATGGCGAAGCATACGGCCACCCACCTGCTGAACTGGGCACTGAGGCAG
ACCCTGGGCCCTGGCACAGAGCAGCAGGGCTCCCATCTCAATCCTGAGCAGCTGCGCTTGGATGTGACC
ACCCAGACCCCATTGACCCCAGAGCAGCTCCGGGCAGTGGAGAACACTGTGCAGGAGGCCGTGGGGCAG
GATGAGGCTGTGTACATGGAGGAGGTGCCCCTGGCGCTCACTGCCCAGGTCCCTGGCCTGCGCTCTCTG
GATGAGGTTTACCCAGACCCTGTGCGGGTGGTATCAGTGGGGGTGCCCGTGGCCCATGCATTGGACCCA
GCCTCCCAAGCCGCACTGCAGACCTCTGTGGAGCTATGCTGTGGGACGCACCTGTTACGTACTGGGGCT
GTAGGGGACCTGGTTATCATCGGGGACCGCCAGCTTTCCAAGGGCACTACCCGCCTGCTGGCCGTCACT
GGGGAGCAGGCCCAGCAGGCCCGAGAGCTAGGCCAGAGCCTGGCCCAGGAAGTGAAAGCGGCCACTGAG
CGGCTGAGTCTGGGGAGCCGGGATGTGGCGGAGGCACTGAGGCTGTCCAAGGACATAGGACGACTCATT
GAAGCTGTGGAAACTGCTGTGATGCCCCAGTGGCAGCGGCGGGAGCTGCTGGCCACAGTGAAGATGCTG
CAGCGGCGTGCCAACACTGCCATCCGTAAGCTGCAAATGGGACAGGCTGCAAAGAAAACTCAGGAGCTG
CTGGAGCGGCACTCGAAGGGGCCTCTGATTGTGGACACAGTCTCTGCTGAGTCTCTCTCAGTGCTGGTG
AAGGTGGTACGGCAGCTGTGTGAGCAGGCCCCCAGCACGTCTGTGCTCCTACTCAGCCCCCAGCCCATG
GGGAAGGTGCTGTGTGCCTGTCAGGTGGCCCAGGGTGCCATGCCCACCTTCACAGCAGAGGCCTGGGCA
CTGGCAGTGTGCAGCCACATGGGGGGCAAGGCGTGGGGCTCACGAGTGGTGGCCCAAGGCACCGGAAGC
ACTACTGACCTGGAAGCTGCCCTCAGTATAGCCCAAACCTATGCCCTCAGCCAGCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_020745
Insert Size 2958 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020745.3
RefSeq Size 4782 bp
RefSeq ORF 2958 bp
Locus ID 57505
UniProt ID Q5JTZ9
Protein Pathways Aminoacyl-tRNA biosynthesis
MW 107.3 kDa
Gene Summary The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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