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WNK4 (NM_032387) Human Untagged Clone
CAT＃: SC305438
WNK4 (untagged)-Human WNK lysine deficient protein kinase 4 (WNK4)
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PHA2B; PRKWNK4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305438 representing NM_032387.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGGCATCCCCGGCCACGGAGACCACCGTCCTCATGTCCCAGACTGAGGCCGACCTGGCCCTGCGG CCCCCGCCTCCTCTTGGCACCGCGGGGCAGCCCCGCCTCGGGCCCCCTCCTCGCCGAGCGCGCCGCTTC TCCGGGAAGGCTGAGCCCCGGCCGCGCTCTTCTCGTCTCAGCCGCCGTAGCTCAGTCGACTTGGGGCTG CTGAGCTCTTGGTCCCTGCCAGCCTCACCCGCTCCGGACCCCCCCGATCCTCCGGACTCCGCTGGTCCT GGCCCCGCGAGGAGCCCACCGCCTAGCTCCAAAGAACCCCCCGAGGGCACGTGGACCGAGGGAGCCCCT GTGAAGGCTGCGGAAGACTCCGCGCGTCCCGAGCTCCCGGACTCTGCAGTGGGCCCGGGGTCCAGGGAG CCGCTAAGGGTCCCTGAAGCTGTGGCCCTAGAGCGGCGGCGGGAGCAGGAAGAAAAGGAGGACATGGAG ACCCAGGCTGTGGCAACGTCCCCCGATGGCCGATACCTCAAGTTTGACATCGAGATTGGACGTGGCTCC TTCAAGACGGTGTATCGAGGGCTAGACACCGACACCACAGTGGAGGTGGCCTGGTGTGAGCTGCAGACT CGGAAACTGTCTAGAGCTGAGCGGCAGCGCTTCTCAGAGGAGGTGGAGATGCTCAAGGGGCTGCAGCAC CCCAACATCGTCCGCTTCTATGATTCGTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTC ACCGAACTCATGACCTCGGGCACGCTCAAGACGTACCTGAGGCGGTTCCGGGAGATGAAGCCGCGGGTC CTTCAGCGCTGGAGCCGCCAAATCCTGCGGGGACTTCATTTCCTACACTCCCGGGTTCCTCCCATCCTG CACCGGGATCTCAAGTGCGACAATGTCTTTATCACGGGACCTACTGGCTCTGTCAAAATCGGGGACCTG GGCCTGGCCACGCTCAAGCGCGCCTCCTTTGCCAAGAGTGTCATCGGGACCCCGGAATTCATGGCCCCC GAGATGTACGAGGAAAAGTACGATGAGGCCGTGGACGTGTACGCGTTCGGCATGTGCATGCTGGAGATG GCCACCTCTGAGTACCCGTACTCCGAGTGCCAGAATGCCGCGCAAATCTACCGCAAGGTCACTTCGGGC AGAAAGCCGAACAGCTTCCACAAGGTGAAGATACCCGAGGTGAAGGAGATCATTGAAGGCTGCATCCGC ACGGATAAGAACGAGAGGTTCACCATCCAGGACCTCCTGGCCCACGCCTTCTTCCGCGAGGAGCGCGGT GTGCACGTGGAACTAGCGGAGGAGGACGACGGCGAGAAGCCGGGCCTCAAGCTCTGGCTGCGCATGGAG GACGCGCGGCGCGGGGGGCGCCCACGGGACAACCAGGCCATCGAGTTCCTGTTCCAGCTGGGCCGGGAC GCGGCCGAGGAGGTGGCACAGGAGATGGTGGCTCTGGGCTTGGTCTGTGAAGCCGATTACCAGCCAGTG GCCCGTGCAGTACGTGAACGGGTTGCTGCCATCCAGCGAAAGCGTGAGAAGCTGCGTAAAGCAAGGGAA TTGGAGGCACTCCCACCAGAGCCAGGACCTCCACCAGCAACTGTGCCCATGGCCCCCGGTCCCCCCAGT GTCTTCCCCCCTGAGCCTGAGGAGCCAGAGGCAGACCAGCACCAGCCCTTCCTTTTCCGCCACGCCAGC TACTCATCTACCACTTCGGATTGCGAGACTGATGGCTACCTCAGCTCCTCCGGCTTCCTGGATGCCTCA GACCCTGCCCTTCAGCCCCCTGGGGGGGTGCCATCCAGCCTGGCTGAGTCCCATCTCTGCCTGCCCTCG GCTTTTGCCCTATCCATTCCACGTTCTGGCCCTGGAAGTGACTTTTCCCCCGGGGACAGCTATGCCTCA GATGCAGCTTCAGGCCTTAGCGATGTGGGAGAAGGGATGGGACAAATGAGGAGACCCCCAGGGAGGAAT CTCCGGCGCAGACCCCGATCCCGGCTGCGGGTCACTAGTGTCTCAGACCAGAATGACAGAGTGGTTGAG TGCCAGCTACAGACCCATAACAGCAAGATGGTGACCTTCCGATTTGATCTGGATGGGGACAGCCCGGAA GAGATTGCAGCTGCCATGGTATATAACGAGTTCATTCTGCCTTCGGAGCGAGATGGATTTCTCAGACGG ATTCGGGAGATTATCCAGCGAGTGGAGACCCTGTTGAAGAGAGACACTGGCCCCATGGAGGCTGCTGAA GACACCCTAAGCCCCCAGGAGGAGCCAGCACCATTACCTGCCCTGCCCGTCCCCCTCCCAGACCCATCC AATGAAGAGCTCCAGAGCAGCACCTCCCTGGAGCACAGGAGCTGGACAGCCTTCTCCACCTCCTCATCT TCTCCTGGAACTCCTTTGTCTCCTGGAAACCCATTTTCCCCTGGAACCCCCATTTCCCCAGGTCCCATC TTCCCCATCACTTCTCCCCCATGTCATCCCAGCCCCTCCCCATTCTCCCCCATTTCTTCCCAGGTCTCC TCAAATCCCTCTCCACACCCCACCAGCTCTCCACTTCCATTCTCCTCCAGCACACCCGAGTTTCCGGTC CCACTCTCTCAGTGTCCCTGGAGTTCTCTCCCCACGACTTCTCCACCTACGTTCTCTCCCACTTGTTCT CAGGTCACTCTTAGTTCCCCTTTCTTTCCTCCGTGCCCCTCCACTTCTTCCTTCCCCTCCACCACAGCA GCCCCTCTCCTTTCTCTGGCTAGTGCCTTCTCACTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCC CCCTCACCTGGGCTCCTTTCCCAGTCTCCTCCAGCCCCTCCTAGTCCCCTCCCTAGCCTGCCCCTTCCC CCTCCCGTTGCTCCTGGTGGCCAGGAAAGCCCTTCACCCCACACAGCTGAGGTGGAGAGTGAGGCCTCA CCACCTCCTGCTCGGCCCCTCCCAGGGGAAGCCAGGCTGGCGCCCATCTCTGAAGAGGGAAAGCCGCAG CTTGTTGGGCGTTTCCAAGTGACTTCATCCAAGGAACCGGCTGAGCCTCTTCCCTTGCAGCCAACATCC CCCACTCTCTCTGGTTCTCCAAAACCTTCAACCCCTCAGCTCACTTCAGAGAGCTCAGATACAGAGGAC AGTGCTGGAGGCGGGCCAGAGACCAGGGAAGCTCTGGCTGAGAGCGACCGTGCAGCTGAGGGTCTGGGG GCTGGAGTTGAGGAGGAAGGAGATGATGGGAAGGAACCCCAAGTTGGGGGCAGCCCCCAACCCCTGAGC CATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCTGTGTTTGAGCAGCGAGGAGTCAGAAAGC AGTGGGGAAGATGAGGAGTTCTGGGCTGAGCTGCAGAGTCTTCGGCAGAAGCACTTGTCAGAGGTGGAA ACACTACAGACACTACAGAAAAAAGAAATTGAAGATTTGTACAGCCGGCTGGGGAAGCAGCCCCCACCG GGTATTGTGGCCCCAGCTGCTATGCTGTCCAGCCGCCAGCGCCGCCTCTCCAAGGGCAGCTTCCCCACC TCCCGCCGCAACAGCCTACAGCGCTCTGAGCCCCCAGGCCCTGGCATCATGCGAAGGAACTCTCTGAGT GGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGACATTCGCCGGGGATGTTGGCAGG ATGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_032387 |
| Insert Size | 3732 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032387.4 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 3732 bp |
| Locus ID | 65266 |
| UniProt ID | Q96J92 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 134.7 kDa |
| Gene Summary | This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.[provided by RefSeq, Sep 2009] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223269 | WNK4 (Myc-DDK-tagged)-Human WNK lysine deficient protein kinase 4 (WNK4) |
CNY 8,656.00 |
|
| RC223269L1 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged |
CNY 11,056.00 |
|
| RC223269L2 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged |
CNY 14,350.00 |
|
| RC223269L3 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), Myc-DDK-tagged |
CNY 14,350.00 |
|
| RC223269L4 | Lenti ORF clone of Human WNK lysine deficient protein kinase 4 (WNK4), mGFP tagged |
CNY 14,350.00 |
|
| RG223269 | WNK4 (tGFP-tagged) - Human WNK lysine deficient protein kinase 4 (WNK4) |
CNY 13,680.00 |
