KRT82 (NM_033033) Human Untagged Clone

CAT#: SC305537

KRT82 (untagged)-Human keratin 82 (KRT82)



  "NM_033033" in other vectors (4)

CNY 8,270.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms Hb-2; HB2; KRTHB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC305537 representing NM_033033.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCGTACCACTCTTTCCAGCCAGGCTCCAGGTGTGGCAGTCAGAGTTTCAGCTCATACTCGGCTGTC
ATGCCCCGGATGGTCACCCACTATGCAGTGAGCAAGGGGCCATGCCGGCCCGGGGGTGGTAGGGGCCTC
CGAGCTCTGGGCTGCCTTGGCTCACGGAGCCTGTGCAACGTGGGCTTTGGGAGGCCCCGGGTAGCCTCC
AGGTGTGGAGGTACCCTGCCTGGCTTCGGGTACCGACTGGGAGCCACCTGTGGGCCTTCTGCCTGCATC
ACCCCTGTCACCATCAATGAGAGCCTGCTGGTCCCACTGGCACTGGAGATAGACCCGACTGTGCAGAGG
GTAAAGAGGGATGAGAAGGAGCAGATCAAGTGCCTCAACAACCGTTTCGCATCTTTCATCAACAAGGTC
CGTTTCCTGGAGCAGAAGAACAAGCTGCTGGAGACCAAGTGGAACTTCATGCAGCAGCAGAGGTGCTGC
CAGACCAACATCGAGCCCATCTTCGAGGGCTATATCAGCGCCCTTCGGCGGCAGCTGGACTGTGTGTCC
GGGGACCGCGTGAGGCTAGAGTCAGAGCTCTGCAGCCTCCAGGCTGCACTGGAGGGCTACAAGAAAAAA
TACGAAGAGGAGCTCTCCCTGCGTCCCTGTGTTGAGAATGAGTTTGTTGCCTTGAAGAAGGACGTGGAC
ACAGCCTTCCTGATGAAGGCTGACCTGGAGACCAACGCAGAGGCACTCGTGCAGGAGATCGACTTCCTG
AAAAGCCTGTATGAGGAGGAGATCTGCCTGCTCCAGTCTCAGATCTCTGAGACCTCGGTCATTGTGAAG
ATGGACAACAGCCGGGAGCTGGACGTGGACGGCATCATCGCTGAGATCAAGGCGCAGTATGACGACATC
GCCAGCCGCAGCAAAGCCGAAGCAGAGGCCTGGTACCAGTGCCGGTATGAGGAGCTGAGAGTCACAGCT
GGGAACCACTGTGACAACCTCCGCAACCGTAAGAACGAGATCCTGGAAATGAATAAACTGATCCAGCGG
CTGCAGCAAGAAACCGAGAATGTCAAAGCCCAGCGCTGCAAACTTGAGGGTGCCATAGCTGAGGCAGAG
CAGCAGGGCGAGGCGGCTCTCAATGATGCCAAGTGCAAGCTGGCAGGGCTGGAGGAGGCTCTGCAGAAG
GCCAAGCAGGACATGGCCTGCCTGCTCAAGGAATATCAGGAGGTGATGAACTCCAAGCTGGGCCTGGAC
ATCGAGATCGCCACCTACAGGCGCCTGCTGGAGGGTGAAGAGCACAGGCTGTGCGAAGGCATCGGGCCC
GTGAATATCTCAGTGAGCAGCTCCAAAGGCGCCTTCCTGTACGAGCCATGTGGGGTCAGCACGCCTGTC
CTCAGCACTGGCGTCCTCAGGAGCAATGGGGGCTGCAGCATCGTGGGCACTGGTGAACTCTATGTCCCC
TGCGAGCCCCAGGGGCTACTGAGCTGTGGGAGCGGGCGGAAATCCAGCATGACGCTAGGAGCTGGGGGC
AGCTCCCCCAGCCACAAGCATTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_033033
Insert Size 1542 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_033033.3
RefSeq Size 2681 bp
RefSeq ORF 1542 bp
Locus ID 3888
UniProt ID Q9NSB4
MW 56.7 kDa
Gene Summary The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this keratin appears to be a hair cuticle-specific keratin. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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