Klotho (KL) (NM_153683) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	klotho; OTTHUMP00000018796
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_153683 edited CGCATGCCCGCCAGCGCCCCGCCGCGCCGCCCGCGGCCGCCGCCGCCGTCGCTGTCGCTG CTGCTGGTGCTGCTGGGCCTGGGCGGCCGCCGCCTGCGTGCGGAGCCGGGCGACGGCGCG CAGACCTGGGCCCGTTTCTCGCGGCCTCCTGCCCCCGAGGCCGCGGGCCTCTTCCAGGGC ACCTTCCCCGACGGCTTCCTCTGGGCCGTGGGCAGCGCCGCCTACCAGACCGAGGGCGGC TGGCAGCAGCACGGCAAGGGTGCGTCCATCTGGGATACGTTCACCCACCACCCCCTGGCA CCCCCGGGAGACTCCCGGAACGCCAGTCTGCCGTTGGGCGCCCCGTCGCCGCTGCAGCCC GCCACCGGGGACGTAGCCAGCGACAGCTACAACAACGTCTTCCGCGACACGGAGGCGCTG CGCGAGCTCGGGGTCACTCACTACCGCTTCTCCATCTCGTGGGCGCGAGTGCTCCCCAAT GGCAGCGCGGGCGTCCCCAACCGCGAGGGGCTGCGCTACTACCGGCGCCTGCTGGAGCGG CTGCGGGAGCTGGGCGTGCAGCCCGTGGTCACCCTGTACCACTGGGACCTGCCCCAGCGC CTGCAGGACGCCTACGGCGGCTGGGCCAACCGCGCCCTGGCCGACCACTTCAGGGATTAC GCGGAGCTCTGCTTCCGCCACTTCGGCGGTCAGGTCAAGTACTGGATCACCATCGACAAC CCCTACGTGGTGGCCTGGCACGGCTACGCCACCGGGCGCCTGGCCCCCGGCATCCGGGGC AGCCCGCGGCTCGGGTACCTGGTGGCGCACAACCTCCTCCTGGCTCATGCCAAAGTCTGG CATCTCTACAATACTTCTTTCCGTCCCACTCAGGGAGGTCAGGTGTCCATTGCCCTAAGC TCTCACTGGATCAATCCTCGAAGAATGACCGACCACAGCATCAAAGAATGTCAAAAATCT CTGGACTTTGTACTAGGTTGGTTTGCCAAACCCGTATTTATTGATGGTGACTATCCCGAG AGCATGAAGAATAACCTTTCATCTATTCTGCCTGATTTTACTGAATCTGAGAAAAAGTTC ATCAAAGGAACTGCTGACTTTTTTGCTCTTTGCTTTGGACCCACCTTGAGTTTTCAACTT TTGGACCCTCACATGAAGTTCCGCCAATTGGAATCTCCCAACCTGAGGCAACTGCTTTCC TGGATTGACCTTGAATTTAACCATCCTCAAATATTTATTGTGGAAAATGGCTGGTTTGTC TCAGGGACCACCAAGAGAGATGATGCCAAATATATGTATTACCTCAAAAAGTTCATCATG GAAACCTTAAAAGCCATCAAGCTGGATGGGGTGGATGTCATCGGGTATACCGCATGGTCC CTCATGGATGGTTTCGAGTGGCACAGAGGTTACAGCATCAGGCGTGGACTCTTCTATGTT GACTTTCTAAGCCAGGACAAGATGTTGTTGCCAAAGTCTTCAGCCTTGTTCTACCAAAAG CTGATAGAGAAAAATGGCTTCCCTCCTTTACCTGAAAATCAGCCCCTAGAAGGGACATTT CCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTACATTCAAGTAAGTCAGCTGACAAAA CCAATCAGCAGTCTCACCAAGCCCTATCACTAATACCACTCTGTCTCAGTTTACCGACCT GAATGTTTACCTGTGGGATGTCCACCACAGTAAAAGGCTTATTAAAGTGGATGGGGTTGT GACCAAGAAGAGGAAATCCTACTGTGTTGACTTTGCTGCCATCCAGCCCCAGATCGCTTT ACTCCAGGAAATGCACGTTACACATTTTCGCTTCTCCCTGGACTGGGCCCTGATTCTCCC TCTGGGTAACCAGTCCCAGGTGAACCACACCATCCTGCAGTACTATCGCTGCATGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_153683 unedited NGGGGTTTGGATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCATGCCCGCCAGCG CCCCGCCGCGCCGCCCGCGGCCGCCGCCGCCGTCGCTGTCGCTGCTGCTGGTGCTGCTGG GCCTGGGCGGCCGCCGCCTGCGTGCGGAGCCGGGCGACGGCGCGCAGACCTGGGCCCGTT TCTCGCGGCCTCCTGCCCCCGAGGCCGCGGGCCTCTTCCAGGGCACCTTCCCCGACGGCT TCCTCTGGGCCGTGGGCAGCGCCGCCTACCAGACCGAGGGCGGCTGGCAGCAGCACGGCA AGGGTGCGTCCATCTGGGATACGTTCACCCACCACCCCCTGGCACCCCCGGGAGACTCCC GGAACGCCAGTCTGCCGTTGGGCGCCCCGTCGCCGCTGCAGCCCGCCACCGGGGACGTAG CCAGCGACAGCTACAACAACGTCTTCCGCGACACGGAGGCGCTGCGCGAGCTCGGGGTCA CTCACTACCGCTTCTCCATCTCGTGGGCGCGAGTGCTCCCCAATGGCAGCGCGGGCGTCC CCAACCGCGAGGGGCTGCGCTACTACCGGCGCCTGCTGGAGCGGCTGCGGGAGCTGGGCG TGCAGCCCGTGGTCACCCTGTACCACTGGGACCTGCCCCAGCGCCTGCAGGACGCCTACG GCGGCTGGGCCAACCGCGCCCTGGCCGACCACTTCAGGGATTACGCGGAGCTCTGCTTCC GCCACTTCGGCGGTCAGGTCAAGTACTGGATCACCATCGACAACCCCTACGTGGTGGCCT GGCACGGCTACGCCACCGGGCGCCTGGCCCCCGGCATCCGGGGCAGCCCGCGGCTCGGGT ACCTGGTGGCGCACAACCTCCTCCTGGCTCATGCCANAGTCTGGCCATCTCTACATACT 3' Read Nucleotide Sequence (showhide) >Forward primer walk for NM_153683 unedited NNGGGGGTGTGCGAGAAGCAAATTTTTTGCTCTTTGCTTTGGNACCCACCTTGNATTTTC AACTTTTGGACCCTCACATGAATTCCGCCAATTGGAATCTCCCAACCTGAGGCAACTGCT TTCCTGGATTGACCTTGAATTTAACCATCCTCAAATATTTATTGTGGAAAATGGCTGGTT TGTCTCAGGGACCACCAAGAGAGATGATGCCAAATATATGTATTACCTCAAAAAGTTCAT CATGGAAACCTTAAAAGCCATCAAGCTGGATGGGGTGGATGTCATCGGGTATACCGCATG GTCCCTCATGGATGGTTTCGAGTGGCACAGAGGTTACAGCATCAGGCGTGGACTCTTCTA TGTTGACTTTCTAAGCCAGGACAAGATGTTGTTGCCAAAGTCTTCAGCCTTGTTCTACCA AAAGCTGATAGAGAAAAATGGCTTCCCTCCTTTACCTGAAAATCAGCCCCTAGAAGGGAC ATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTACATTCAAGTAAGTCAGCTGAC AAAACCAATCAGCAGTCTCACCAAGCCCTATCACTAATACCACTCTGTCTCAGTTTACCG ACCTGAATGTTTACCTGTGGGATGTCCACCACAGTAAAAGGCTTATTAAAGTGGATGGGG TTGTGACCAAGAAGAGGAAATCCTACTGTGTTGACTTTGCTGCCATCCAGCCCCAGATCG CTTTACTCCAGGAAATGCACGTTACACATTTTCGCTTCTCCCTGGACTGGGCCCTGATTC TCCCTCTGGGTAACCAGTCCCAGGTGAACCACACCATCCTGCAGTACTATCGCTGCATGG CCAGCGAGCTTTGTCGTGTCAACTCACCCCAGTGGTG
Restriction Sites	Please inquire
ACCN	NM_153683
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153683.2, NP_710150.1
RefSeq Size	5049 bp
RefSeq ORF	5049 bp
Locus ID	9365
Protein Families	Secreted Protein, Transmembrane
Gene Summary	This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains an additional segment within the coding region, which results in early translation termination, compared to variant 1. The resulting soluble isoform (b) contains a shorter C-terminus compared to isoform a.

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