KRT27 (NM_181537) Human Untagged Clone
CAT#: SC307304
KRT27 (untagged)-Human keratin 27 (KRT27)
CNY 7,320.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | K25IRS3; KRT25C |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC307304 representing NM_181537.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGTGCGCTTTTCTTCTACCTCCAGGAGACTTGGCTCTTGCGGGGGCACTGGCTCTGTGAGGCTC TCTAGTGGGGGAGCAGGCTTTGGGGCTGGAAACACATGCGGTGTGCCAGGCATTGGAAGTGGCTTCTCT TGTGCTTTTGGGGGCAGCTCATCTGCAGGAGGCTATGGCGGAGGTCTGGGCGGGGGAAGTGCTTCCTGT GCTGCCTTCACAGGGAATGAGCACGGCCTCCTCTCTGGCAATGAGAAGGTGACCATGCAGAACCTCAAC GACCGCTTGGCCTCCTACCTGGAGAATGTTCGAGCCCTAGAGGAGGCCAACGCTGACTTGGAGCAGAAG ATCAAGGGGTGGTATGAGAAATTTGGACCTGGTTCTTGCCGTGGCCTTGATCATGATTACAGCAGATAT TTCCCAATTATTGACGAACTTAAGAACCAGATAATTTCTGCAACTACCAGTAATGCCCATGTTGTCCTG CAAAATGATAATGCAAGACTAACAGCTGATGACTTCAGACTAAAGTTTGAAAACGAGCTAGCGCTTCAC CAGAGCGTGGAGGCGGACATCAATGGTTTGCGAAGAGTCCTGGATGAGCTGACCTTGTGCAGAACGGAC CTGGAGATCCAGCTGGAAACTCTCAGTGAGGAGCTCGCTTACCTCAAGAAGAATCATGAGGAGGAAATG AAAGCTCTTCAGTGCGCGGCTGGAGGCAACGTGAACGTGGAGATGAACGCGGCCCCCGGGGTAGACCTC ACGGTTCTGCTGAACAATATGCGAGCTGAGTACGAAGCCCTCGCAGAGCAGAACCGCAGGGACGCGGAG GCCTGGTTCAACGAAAAGAGCGCCTCGCTGCAGCAGCAGATCTCTGACGACGCTGGCGCCACCACCTCA GCCCGGAATGAGCTTATCGAGATGAAACGCACTCTTCAAACCCTTGAGATTGAACTTCAGTCCCTCTTA GCAACGAAACACTCCCTGGAGTGCTCCTTGACAGAGACCGAGAGTAACTACTGTGCACAGCTGGCACAG ATCCAGGCTCAGATCGGGGCCCTGGAGGAGCAGCTGCACCAGGTCAGAACCGAGACCGAGGGCCAGAAG CTCGAGTATGAGCAGCTCCTTGACATCAAGGTCCACCTGGAAAAAGAAATTGAGACCTACTGCCTCCTG ATAGATGGAGAAGATGGCTCCTGTTCTAAATCAAAAGGCTATGGAGGCCCAGGAAATCAAACAAAAGAT TCATCTAAAACCACCATTGTCAAAACAGTTGTTGAAGAGATAGATCCTCGTGGCAAAGTTCTCTCATCC AGAGTTCACACTGTGGAAGAGAAATCCACCAAAGTCAACAACAAGAATGAACAGAGGGTGTCTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_181537 |
Insert Size | 1380 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_181537.3 |
RefSeq Size | 1612 bp |
RefSeq ORF | 1380 bp |
Locus ID | 342574 |
UniProt ID | Q7Z3Y8 |
MW | 49.8 kDa |
Gene Summary | This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221911 | KRT27 (Myc-DDK-tagged)-Human keratin 27 (KRT27) |
CNY 3,656.00 |
|
RC221911L3 | Lenti ORF clone of Human keratin 27 (KRT27), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC221911L4 | Lenti ORF clone of Human keratin 27 (KRT27), mGFP tagged |
CNY 5,890.00 |
|
RG221911 | KRT27 (tGFP-tagged) - Human keratin 27 (KRT27) |
CNY 4,370.00 |