GARNL1 (RALGAPA1) (NM_194301) Human Untagged Clone
CAT#: SC307589
RALGAPA1 (untagged)-Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2
CNY 40,090.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1; TULIP1 |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_194301, the custom clone sequence may differ by one or more nucleotides
ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACC AAGAAGGACGCACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCT ATTGATCTTAAACAGTTTTTCGACCAACATTTTTCACATATATACTATGTGTTCTTTGAA AATTTTGTGACTATTGAAGCTAGTCTTAAACAGAAAGGTCACAAGTCTCAAAGAGAGGAA TTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAACTTCTTCCAGAAAGAATTCAT CAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCACACAGGAAAC TCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCA CCACAGTCTGAACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTT CAAGAAACTCAAGTCACTATAGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGAT AAAGGGCAAGAAGATCTCACAAGCTATTTTCTTGAAGCACTTCTAAAATACATAGTCATT CAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAGAAAGGGGATTTTCATTTTTG TTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTGTAAGGAAAAC AGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAG GCTCGTGTTATTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACA GGACCTCATATTCCTGGGATGGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCT GCTAGTTTAGTATCCAGAGAAGAAAGCAAAAATGATAATGCTGATAAAACAGACAGAACT ACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCACGGAGCGAGAACCTAGCTCA TCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGTTCGCAGAGTT TTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATC CAACAAGAGGAAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCA GACCTCCCTTGCATTGAAAATGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAA AGAGAAGAGGAAAATGGGACCAATACTGCTGATCATGTTCGAAATTCCAGTTGGGCAAAA AACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAGAAGCCACAGAACAAAACATA CGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAATATATTTCTT CTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAA CAGATGCTGCTTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCT TTTCTACAGTTCCAAGGGAAAAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTT TTCCAGACCCTTATAGTTGCCTGGATCAAAGCAAACCTAAATGTGTACATCTCCCGAGAA CTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCTATTGGGAAGAGTTGGCCACT GAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTTATATAGTTTG GATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGG AGTCGTGATCAGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCT CCAGGAACCGAAAAGGCGAGGAGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCT CAAATACTTCCCCGCTCAACTAGAGTCAGACATTTTTCACAAAGTGAAGAAACTGGAAAT GAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTCGAAGTAGCAGCACTTCTGAC ATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGAGGACATGAGCCAAAAA CTGCCTCCTCTTAATAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGAT GAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGT AGTCCAGGCAGCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAG ATGCGCCCTATTGATGACCCAGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGG AGCAGTGATCTTATCAGCTCAGATAGTCATTCGGATTCTTTCAGCGCTTTCCAATATGAT GGCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACTGGGGTTACGTCCTCTGCTGAT GTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGGCTAGTCTAACT ACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTT GCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGGTCA CAGACTCCTTCCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTC GACGAGAAGCTCCACCACTCTGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTT CCATCAGAATGTTGTAGTGTGATGGCAGGAGGTACTCTGACTGGATGGCATGCTGATGTT GCTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGAGATGTAAATTCAATCATGGAT CCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCTAGCTAAG ATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTTA ATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAA TATAAACAAGGTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAA GATGTTTCTCCAAATAGAGATTTTCTAACACATTTCTACAATATAATGCATTGTGGATTA CTTCATATTGACCAGGATATTGTCAATACAATCATCAAACACTGCTCACCTCAATTTTTT TCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATTTTATTGTAGCAGCTGGTAGA GTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTCTTCTGGGATCT TTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGAT GTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCG GCAAGAGATGAGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGTATTTGG ATTTGTGAAGAACTAGTCCATGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTG ATTTGTGTTTCCTTAAAGTTTACTAATAAAACAGTAGCCCACGTAGCTTGTAACATGCTT CACATGCTGGTTCATTATGTACCTAGACTTCAGATTTACCAGCCTGATTCTCCCTTGAAA ATTATTCAAATCCTAATAGCTACCATCACCCATCTTTTACCAAGTACAGAGGCTTCATCT TATGAAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCATG GCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGGAGCAGAAAGCGATAAA ACAGAAAAATCTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGTGTTTATGGAGCT CAGTGTTTTAGCAATCCAAGGTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGAT TATGATCCTTTTATGCATTTGGAAAGTCTGAAAGAGCCTGAGCCTCTGCACTCTCCTGAC TCAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGAAAACTCAAATGCAGCATGGA TTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAATCACCTGGGCCATTAT CCAATGAGCGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCAT TACAGTGAAAGTACTGAACTTTCTCCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTT GTGTTAAATAATACAACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCT GGAGGAGGTTTATCTGCTGGCCTTGCATCAGCCAATTCAAATGTCAGAATCATAGTACGT GATCTCTCTGGAAAATATTCATGGGATTCTGCTATACTGTATGGCCCACCTCCTGTAAGT GGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTCACCAAGAGAAGCCAGAAGAG CCTCCGACATCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACTTTCTCTCCAG TTTAAAAGATTTAGAGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTT CTTGATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGAACTGGA ATCTCACTTAATATTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAAGAAAATGAT GTTATTAATGCTATCCTTAAGCAACATACAGAAGAAAAAGAATTTGTTGAGAAGCACTTT AATGACTTAAACATGAAAGCTGTGGAACAAGATGAACCAATACCTCAAAAACCTCAGTCA GCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAATGAATTCCTGGGACAAACGG AGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAGGAACTTGGAT TCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAA GACAAACACTCCATTCTCACCAATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCT GGTCTTGGTTGGGAGGTAAATCTTACAAACCATTGTGGTTTTATGGGAGGACTACAAAAA AACAAAAGCACTGGATTGACCACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTT CACGTGTCAACAAGAATGCCTTCTGATTCTGATGATTCTTTGACCAAAAAATTGAGACAT TTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATACTAGAGACTACAGGAGAGGA ATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAAAAATCACATG TTCAGTATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGT GCTATTGTGAATGGAAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGC CGTGCTCTGAAATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCACGATAC CTGCAAACAATTGTCCAGCACCACTTAGAACCAACAACATTTGAAGATTTTGCAGCACAG GTTTTTTCTCCAGCTCCCTACCACCATTTACCATCTGATGCCGGCTCCTACCCAGAGATT CTACCCAGTGAAACTCCCACAGCAACGCAGGTAGATGGGGCTGACCTGGCCTCTCCAATG TCTCCTCGAACTAGCAAAAGCCGCATGTCCATGAAGCTGCGTCGTTCCTCTGGCTCAGCC AATAAATCCTAA |
Restriction Sites | Please inquire |
ACCN | NM_194301 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_194301.2, NP_919277.2 |
RefSeq Size | 7851 bp |
RefSeq ORF | 6252 bp |
Locus ID | 253959 |
UniProt ID | Q6GYQ0 |
Gene Summary | This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (2) differs in the 3' UTR and lacks an alternate exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is longer and has a distinct C-terminus, compared to isoform 1. Isoforms 2 and 4 are the same length, but have distinct protein sequences. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221378 | RALGAPA1 (Myc-DDK-tagged)-Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2 |
CNY 13,752.00 |
|
RG221378 | RALGAPA1 (tGFP-tagged) - Human Ral GTPase activating protein, alpha subunit 1 (catalytic) (RALGAPA1), transcript variant 2 |
CNY 20,900.00 |