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TRIAD3 (RNF216) (NM_207116) Human Untagged Clone
CAT＃: SC308390
RNF216 (untagged)-Human ring finger protein 216 (RNF216), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAHH; TRIAD3; U7I1; UBCE7IP1; ZIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308390 representing NM_207116.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGAGGGAAACAACAATGAAGAGGTAATTCACTTGAACAACTTTCACTGCCATCGGGGACAAGAG TGGATCAATCTCCGAGATGGGCCCATCACCATATCTGACTCCTCAGATGAGGAAAGGATTCCAATGCTG GTCACCCCAGCTCCTCAGCAGCATGAAGAAGAGGACCTGGATGATGATGTCATCCTGACAGAAGATGAT TCTGAGGATGACTACGGTGAATTTCTGGATCTTGGGCCTCCTGGAATCTCTGAATTCACTAAGCCAAGT GGCCAAACAGAAAGAGAACCCAAGCCTGGACCGAGTCATAACCAAGCAGCAAATGACATTGTCAACCCC AGATCAGAGCAGAAAGTCATCATCTTGGAAGAAGGTAGCCTTCTTTACACAGAAAGCGATCCTTTGGAA ACTCAGAACCAGTCATCCGAAGACTCAGAGACAGAGCTGTTATCAAATCTAGGAGAGTCAGCTGCTCTA GCAGATGATCAGGCCATCGAAGAAGACTGCTGGTTAGATCATCCTTACTTCCAGTCTCTGAACCAACAG CCCCGTGAAATAACAAACCAGGTCGTTCCTCAGGAACGGCAGCCTGAAGCAGAACTGGGCCGCTTGTTG TTTCAGCATGAATTCCCAGGGCCCGCTTTTCCAAGGCCGGAACCCCAGCAAGGTGGGATTTCAGGCCCC TCTTCTCCTCAGCCTGCCCATCCTCTAGGAGAGTTTGAAGACCAGCAGTTAGCAAGTGATGATGAAGAG CCAGGTCCAGCCTTTCCAATGCAAGAATCTCAAGAGCCCAATTTGGAAAACATTTGGGGGCAAGAAGCT GCAGAGGTAGATCAAGAGCTCGTTGAACTACTAGTGAAAGAAACGGAAGCAAGATTTCCAGATGTAGCA AATGGGTTTATTGAGGAAATAATTCATTTTAAGAATTATTATGATCTGAATGTACTTTGTAATTTTCTT CTGGAAAACCCAGATTATCCAAAGAGAGAAGACAGAATCATTATAAATCCCAGTAGCAGTCTGCTGGCC AGCCAAGATGAGACAAAGTTGCCTAAAATAGACTTTTTTGACTATTCTAAATTGACCCCTCTTGACCAG CGCTGCTTCATCCAAGCTGCTGACCTCCTCATGGCCGACTTCAAAGTGCTCAGTAGTCAGGACATCAAG TGGGCCCTGCACGAGCTCAAAGGACACTATGCAATCACCCGAAAGGCCTTGTCTGATGCCATTAAAAAA TGGCAGGAGCTGTCACCAGAAACCAGTGGAAAAAGGAAGAAGAGAAAACAAATGAACCAGTATTCTTAC ATTGATTTCAAGTTTGAACAAGGTGACATAAAAATAGAAAAGAGGATGTTCTTTCTTGAAAATAAGCGA CGACATTGTAGGTCCTATGACCGACGTGCTCTCCTTCCAGCTGTGCAACAAGAGCAGGAGTTCTATGAG CAGAAAATCAAAGAGATGGCAGAGCATGAAGACTTTTTGCTTGCCCTACAGATGAATGAAGAACAGTAT CAAAAGGATGGCCAGCTGATTGAGTGTCGCTGCTGCTATGGGGAATTTCCATTCGAGGAGCTGACGCAG TGCGCAGATGCTCACTTGTTCTGCAAAGAGTGTCTCATCAGATATGCCCAAGAGGCAGTCTTTGGATCT GGAAAGTTGGAGCTCAGCTGCATGGAAGGCAGCTGCACGTGTTCGTTCCCAACCAGTGAGCTGGAGAAG GTGCTCCCCCAGACCATCCTGTATAAGTACTATGAGCGAAAAGCCGAGGAGGAGGTTGCGGCAGCCTAC GCCGACGAGCTTGTCAGGTGCCCGTCCTGTAGCTTTCCGGCTCTGTTGGACAGTGATGTGAAGAGGTTC AGCTGTCCTAATCCTCACTGCCGAAAGGAAACCTGTAGGAAGTGTCAGGGACTCTGGAAAGAACATAAT GGCCTCACCTGTGAAGAGCTGGCTGAAAAAGACGACATCAAGTACCGTACCTCTATTGAAGAAAAAATG ACTGCTGCCCGCATTAGAAAATGCCACAAGTGTGGGACTGGCCTCATCAAATCTGAAGGCTGCAACCGC ATGTCTTGCCGCTGTGGTGCCCAGATGTGCTACCTCTGTCGAGTTTCTATTAATGGATATGACCATTTC TGCCAACATCCCCGCTCACCAGGAGCCCCTTGCCAGGAGTGTTCAAGATGCTCTCTCTGGACCGATCCC ACTGAAGATGATGAGAAGCTTATTGAGGAAATCCAGAAGGAGGCTGAAGAGGAACAGAAAAGAAAGAAT GGAGAGAACACCTTCAAACGCATTGGACCCCCGCTGGAGAAGCCTGTGGAGAAGGTGCAGAGGGTGGAG GCCCTCCCGAGGCCCGTTCCGCAGAACCTGCCACAGCCACAGATGCCACCCTATGCCTTCGCGCACCCA CCCTTCCCCCTGCCTCCCGTGCGGCCTGTGTTCAACAACTTCCCACTCAACATGGGGCCTATCCCAGCC CCGTACGTGCCCCCTCTGCCCAACGTGCGGGTCAACTATGACTTCGGTCCCATCCACATGCCCCTGGAG CACAACCTGCCCATGCACTTTGGCCCCCAGCCGCGGCATCGCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_207116 |
| Insert Size | 2601 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_207116.2 |
| RefSeq Size | 5696 bp |
| RefSeq ORF | 2601 bp |
| Locus ID | 54476 |
| UniProt ID | Q9NWF9 |
| MW | 99.4 kDa |
| Gene Summary | This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2, also known as TRIAD3A) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209430 | RNF216 (Myc-DDK-tagged)-Human ring finger protein 216 (RNF216), transcript variant 2 |
CNY 6,344.00 |
|
| RC209430L1 | Lenti ORF clone of Human ring finger protein 216 (RNF216), transcript variant 2, Myc-DDK-tagged |
CNY 8,744.00 |
|
| RC209430L2 | Lenti ORF clone of Human ring finger protein 216 (RNF216), transcript variant 2, mGFP tagged |
CNY 8,744.00 |
|
| RC209430L3 | Lenti ORF clone of Human ring finger protein 216 (RNF216), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC209430L4 | Lenti ORF clone of Human ring finger protein 216 (RNF216), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG209430 | RNF216 (tGFP-tagged) - Human ring finger protein 216 (RNF216), transcript variant 2 |
CNY 7,944.00 |
