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PKN1 (NM_213560) Human Untagged Clone
CAT＃: SC308656
PKN1 (untagged)-Human protein kinase N1 (PKN1), transcript variant 1
CNY 18,340.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308656 representing NM_213560.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAGGCCAATAACCCCTCGGAGCAGGAGCTGGAGAGTGAGCCTCGCAGCTGGTCCCTGCTAGAG CAGCTGGGCCTGGCCGGGGCAGACCTGGCGGCCCCCGGGGTACAGCAGCAGCTGGAGCTGGAGCGGGAG CGGCTGCGGCGGGAAATCCGCAAGGAGCTGAAGCTGAAGGAGGGTGCTGAGAACCTGCGGCGGGCCACC ACTGACCTGGGCCGCAGCCTGGGCCCCGTAGAGCTGCTGCTGCGGGGCTCCTCGCGCCGCCTCGACCTG CTGCACCAGCAGCTGCAGGAGCTGCACGCCCACGTGGTGCTTCCCGACCCGGCGGCCACCCACGATGGC CCCCAGTCCCCTGGTGCGGGTGGCCCCACCTGCTCGGCCACCAACCTGAGCCGCGTGGCGGGCCTGGAG AAGCAGTTGGCCATTGAGCTGAAGGTGAAGCAGGGGGCGGAGAACATGATCCAGACCTACAGCAATGGC AGCACCAAGGACCGGAAGCTGCTGCTGACAGCCCAGCAGATGTTGCAGGACAGTAAGACCAAGATTGAC ATCATCCGCATGCAACTCCGCCGGGCGCTGCAGGCCGGCCAGCTGGAGAACCAGGCAGCCCCGGATGAC ACCCAAGGGAGTCCTGACCTGGGGGCTGTGGAGCTGCGCATCGAAGAGCTGCGGCACCACTTCCGAGTG GAGCACGCGGTGGCCGAGGGTGCCAAGAACGTACTGCGCCTGCTCAGCGCTGCCAAGGCCCCGGACCGC AAGGCAGTCAGCGAGGCCCAGGAGAAATTGACAGAATCCAACCAGAAGCTGGGGCTGCTGCGGGAGGCT CTGGAGCGGAGACTTGGGGAGCTGCCCGCCGACCACCCCAAGGGGCGGCTGCTGCGAGAAGAGCTCGCT GCGGCCTCCTCCGCTGCCTTCAGCACCCGCCTGGCCGGGCCCTTTCCCGCCACGCACTACAGCACCCTG TGCAAGCCCGCGCCGCTCACAGGGACCCTGGAGGTACGAGTGGTGGGCTGCAGAGACCTCCCAGAGACC ATCCCGTGGAACCCTACCCCCTCAATGGGGGGACCTGGGACCCCAGACAGCCGCCCCCCCTTCCTGAGC CGCCCAGCCCGGGGCCTTTACAGCCGAAGCGGAAGCCTCAGTGGCCGGAGCAGCCTCAAAGCAGAAGCC GAGAACACCAGTGAAGTCAGCACTGTGCTTAAGCTGGATAACACAGTGGTGGGGCAGACGTCTTGGAAG CCATGTGGCCCCAATGCCTGGGACCAGAGCTTCACTCTGGAGCTGGAAAGGGCACGGGAACTGGAGTTG GCTGTGTTCTGGCGGGACCAGCGGGGCCTGTGTGCCCTCAAATTCCTGAAGTTGGAGGATTTCTTGGAC AATGAGAGGCATGAGGTGCAGCTGGACATGGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACCTTCCGC AACCCTGTCATTGAGAGGATTCCTCGGCTCCGACGGCAGAAGAAAATTTTCTCCAAGCAGCAAGGGAAG GCGTTCCAGCGTGCTAGGCAGATGAACATCGATGTCGCCACGTGGGTGCGGCTGCTCCGGAGGCTCATC CCCAATGCCACGGGCACAGGCACCTTTAGCCCTGGGGCTTCTCCAGGATCCGAGGCCCGGACCACGGGT GACATATCGGTGGAGAAGCTGAACCTCGGCACTGACTCGGACAGCTCACCTCAGAAGAGCTCGCGGGAT CCTCCTTCCAGCCCATCGAGCCTGAGCTCCCCCATCCAGGAATCCACTGCTCCCGAGCTGCCTTCGGAG ACCCAGGAGACCCCAGGCCCCGCCCTGTGCAGCCCTCTGAGGAAGTCACCTCTGACCCTCGAAGATTTC AAGTTCCTGGCGGTGCTGGGCCGGGGTCATTTTGGGAAGGTGCTCCTCTCCGAATTCCGGCCCAGTGGG GAGCTGTTCGCCATCAAGGCTCTGAAGAAAGGGGACATTGTGGCCCGAGACGAGGTGGAGAGCCTGATG TGTGAGAAGCGGATATTGGCGGCAGTGACCAGTGCGGGACACCCCTTCCTGGTGAACCTCTTCGGCTGT TTCCAGACACCGGAGCACGTGTGCTTCGTGATGGAGTACTCGGCCGGTGGGGACCTGATGCTGCACATC CACAGCGACGTGTTCTCTGAGCCCCGTGCCATCTTTTATTCCGCCTGCGTGGTGCTGGGCCTACAGTTT CTTCACGAACACAAGATCGTCTACAGGGACCTGAAGTTGGACAATTTGCTCCTGGACACCGAGGGCTAC GTCAAGATCGCAGACTTTGGCCTCTGCAAGGAGGGGATGGGCTATGGGGACCGGACCAGCACATTCTGT GGGACCCCGGAGTTCCTGGCCCCTGAGGTGCTGACGGACACGTCGTACACGCGAGCTGTGGACTGGTGG GGACTGGGTGTGCTGCTCTACGAGATGCTGGTTGGCGAGTCCCCATTCCCAGGGGATGATGAGGAGGAG GTCTTCGACAGCATCGTCAACGACGAGGTTCGCTACCCCCGCTTCCTGTCGGCCGAAGCCATCGGCATC ATGAGAAGGCTGCTTCGGAGGAACCCAGAGCGGAGGCTGGGATCTAGCGAGAGAGATGCAGAAGATGTG AAGAAACAGCCCTTCTTCAGGACTCTGGGCTGGGAAGCCCTGTTGGCCCGGCGCCTGCCACCGCCCTTT GTGCCCACGCTGTCCGGCCGCACCGACGTCAGCAACTTCGACGAGGAGTTCACCGGGGAGGCCCCCACA CTGAGCCCGCCCCGCGACGCGCGGCCCCTCACAGCCGCGGAGCAGGCAGCCTTCCTGGACTTCGACTTC GTGGCCGGGGGCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_213560 |
| Insert Size | 2847 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213560.1 |
| RefSeq Size | 2964 bp |
| RefSeq ORF | 2847 bp |
| Locus ID | 5585 |
| UniProt ID | Q16512 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 104.7 kDa |
| Gene Summary | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215735 | PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 10,416.00 |
|
| RC215735L1 | Lenti-ORF clone of PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 12,816.00 |
|
| RC215735L2 | Lenti-ORF clone of PKN1 (mGFP-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| RC215735L3 | Lenti-ORF clone of PKN1 (Myc-DDK-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| RC215735L4 | Lenti-ORF clone of PKN1 (mGFP-tagged)-Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 9,030.00 |
|
| RG215735 | PKN1 (tGFP-tagged) - Human protein kinase N1 (PKN1), transcript variant 1 |
CNY 7,890.00 |
