ALS2 (NM_020919) Human Untagged Clone

CAT#: SC308723

ALS2 (untagged)-Human amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant 1



  "NM_020919" in other vectors (4)

CNY 17,016.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-ALS2 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ALS2CR6; ALSJ; IAHSP; PLSJ
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC308723 representing NM_020919.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACTCAAAGAAGAGAAGCTCAACAGAGGCAGAAGGATCCAAGGAAAGAGGCCTGGTCCATATCTGG
CAGGCAGGATCCTTTCCCATAACACCAGAGAGATTGCCAGGCTGGGGAGGAAAGACTGTTTTGCAGGCA
GCCCTCGGAGTGAAACATGGAGTTCTTCTGACTGAAGATGGTGAGGTCTACAGCTTTGGGACTCTTCCC
TGGAGAAGTGGACCAGTGGAGATTTGTCCAAGTAGCCCCATTCTAGAAAATGCCCTGGTTGGGCAATAT
GTTATTACTGTGGCAACAGGAAGCTTCCATAGTGGAGCAGTGACAGACAATGGTGTCGCGTACATGTGG
GGAGAGAATTCTGCTGGCCAGTGTGCAGTAGCCAACCAGCAGTATGTGCCGGAACCAAATCCTGTCAGC
ATTGCTGATTCTGAGGCCAGCCCTTTGTTAGCAGTCAGGATTTTACAGTTGGCGTGTGGCGAGGAGCAC
ACTCTGGCATTGTCAATAAGCAGAGAGATTTGGGCATGGGGTACCGGTTGTCAGTTGGGTCTCATTACC
ACTGCCTTCCCAGTGACAAAGCCGCAAAAGGTAGAACATCTTGCTGGGCGAGTGGTGCTTCAAGTTGCC
TGTGGTGCTTTCCACAGCTTAGCCCTTGTACAATGCCTCCCTTCCCAGGATCTGAAGCCAGTCCCAGAA
CGATGCAACCAGTGCAGCCAGCTCTTGATTACTATGACTGACAAAGAAGACCATGTGATTATATCAGAC
AGTCATTGTTGCCCATTAGGTGTGACACTGACAGAATCTCAGGCAGAAAACCATGCCAGCACTGCTCTC
AGCCCCTCCACTGAAACCCTTGACAGGCAGGAAGAAGTATTTGAGAACACTCTTGTAGCAAATGATCAG
TCTGTTGCTACTGAACTGAATGCAGTAAGTGCTCAGATCACAAGCAGCGATGCCATGTCCTCTCAACAA
AATGTCATGGGAACAACTGAAATTTCCTCTGCCAGAAACATACCATCATACCCTGACACCCAAGCAGTC
AATGAATACCTACGGAAACTGTCAGATCATTCAGTAAGAGAGGACTCAGAGCATGGTGAAAAGCCAGTG
CCATCTCAGCCTCTTTTAGAAGAAGCAATTCCTAATCTCCACAGCCCGCCTACCACAAGCACCTCAGCC
CTAAACAGCCTGGTGGTCTCTTGTGCATCTGCTGTTGGTGTGAGAGTGGCTGCTACTTATGAAGCTGGT
GCCTTGTCACTGAAGAAAGTTATGAACTTTTATAGTACAACCCCTTGTGAAACTGGAGCTCAGGCAGGC
AGTAGTGCCATTGGCCCCGAAGGTTTGAAAGATAGCAGGGAAGAACAGGTTAAACAGGAATCAATGCAA
GGAAAGAAAAGTTCAAGTCTTGTGGATATCAGAGAAGAAGAAACAGAGGGAGGCAGTCGAAGACTCTCC
CTCCCTGGATTGTTGTCACAAGTTTCCCCCAGGCTCTTAAGAAAGGCTGCACGGGTGAAAACGAGGACA
GTGGTTCTGACCCCCACATACAGTGGAGAAGCAGATGCGCTCCTGCCTTCTCTGAGAACAGAAGTGTGG
ACCTGGGGGAAAGGGAAGGAAGGGCAGCTGGGGCACGGCGATGTTCTGCCTAGGCTTCAACCGTTGTGT
GTAAAATGTCTGGATGGCAAAGAAGTAATCCATCTGGAGGCAGGTGGTTACCATTCTCTTGCACTTACT
GCGAAATCCCAGGTTTACTCATGGGGTAGCAATACCTTTGGTCAACTTGGGCATTCCGATTTTCCAACA
ACAGTTCCTCGTCTTGCAAAGATAAGCAGTGAAAATGGAGTCTGGAGCATAGCTGCAGGCAGGGATTAT
TCCCTGTTTTTAGTGGATACAGAAGACTTCCAGCCTGGGTTATATTACAGTGGCCGACAGGACCCTACA
GAAGGTGACAACCTTCCAGAGAATCACAGTGGTTCTAAGACTCCAGTACTTCTCTCCTGTAGTAAGCTT
GGATATATAAGCAGAGTGACAGCAGGAAAAGATAGCTATTTAGCCTTGGTGGATAAAAACATTATGGGG
TATATTGCCAGTCTCCACGAGTTAGCTACTACAGAAAGACGATTCTATTCAAAACTAAGTGATATCAAA
TCTCAGATTCTCAGGCCTCTTCTCAGTTTAGAAAATTTGGGCACTACAACTACAGTCCAGCTGTTGCAG
GAGGTGGCTAGCCGATTCAGCAAGCTGTGTTACCTCATTGGTCAGCATGGAGCCTCATTGAGCAGCTTC
CTTCATGGGGTAAAGGAAGCCAGGAGTTTGGTCATCCTGAAGCATTCAAGTCTCTTCTTGGATAGTTAT
ACAGAGTATTGCACATCTATTACAAATTTCCTGGTTATGGGAGGATTCCAGCTTCTTGCTAAGCCTGCC
ATTGATTTCCTAAATAAAAACCAAGAGCTGTTGCAAGATTTGTCAGAAGTGAATGACGAAAACACTCAG
TTGATGGAAATACTGAATACTTTGTTTTTCTTGCCAATCAGACGACTTCATAATTACGCAAAAGTTTTG
CTAAAGCTTGCTACTTGTTTTGAAGTGGCATCTCCAGAATATCAGAAACTGCAGGATTCCAGTTCTTGT
TATGAGTGTCTTGCTCTCCATCTCGGCAGGAAAAGGAAGGAAGCAGAATACACACTGGGCTTCTGGAAG
ACCTTCCCCGGAAAAATGACGGATTCCTTGAGGAAGCCAGAGCGTCGACTGCTGTGTGAGAGTAGTAAC
CGAGCCCTGTCTCTGCAGCATGCTGGGAGGTTTTCCGTGAATTGGTTCATTCTCTTTAATGATGCCCTG
GTCCATGCCCAGTTCTCCACGCACCATGTTTTCCCTCTGGCCACGCTGTGGGCAGAGCCACTGTCTGAA
GAAGCTGGTGGTGTGAATGGCTTAAAGATAACTACACCTGAGGAGCAGTTCACTCTCATTTCATCTACA
CCCCAGGAAAAGACAAAGTGGCTACGAGCTATAAGCCAAGCCGTAGATCAGGCTTTGAGAGGGATGTCT
GATCTCCCCCCTTATGGAAGTGGTAGCAGTGTTCAGAGACAGGAACCACCCATTTCACGCAGTGCCAAA
TATACTTTCTACAAGGATCCTCGCCTAAAGGATGCCACCTATGATGGACGCTGGCTTTCAGGGAAGCCT
CATGGCAGAGGGGTTTTGAAGTGGCCTGATGGAAAGATGTATTCTGGCATGTTCAGGAATGGCTTGGAA
GATGGGTATGGAGAATACAGAATCCCAAACAAGGCAATGAACAAAGAAGACCATTATGTGGGCCATTGG
AAAGAAGGAAAAATGTGCGGTCAAGGAGTCTACAGCTATGCTTCTGGTGAAGTATTTGAGGGCTGTTTT
CAAGATAATATGCGTCATGGTCATGGTCTTCTACGAAGTGGGAAATTGACGTCCTCTTCTCCTAGTATG
TTCATTGGCCAGTGGGTAATGGATAAGAAAGCAGGATATGGTGTCTTTGATGATATCACTAGGGGGGAA
AAGTATATGGGAATGTGGCAAGATGATGTGTGTCAAGGGAATGGTGTGGTGGTTACCCAGTTTGGATTA
TACTACGAGGGCAACTTTCACCTTAATAAAATGATGGGAAATGGGGTTTTGCTTTCCGAAGATGATACT
ATCTATGAAGGAGAATTTTCAGATGACTGGACTCTTAGTGGAAAGGGAACACTGACTATGCCAAATGGA
GACTACATTGAAGGTTATTTTAGTGGAGAATGGGGATCTGGGATAAAAATCACTGGAACCTACTTCAAA
CCTAGTCTATATGAGAGTGATAAAGACAGACCTAAAGTTTTCAGGAAGCTAGGAAACCTGGCAGTGCCA
GCTGATGAGAAGTGGAAAGCGGTGTTTGACGAATGTTGGCGCCAACTGGGCTGTGAGGGCCCAGGCCAA
GGGGAAGTTTGGAAAGCATGGGACAATATTGCTGTGGCCTTGACCACCAGTCGGCGCCAGCACAGAGAC
AGTCCAGAAATACTGAGTCGTTCACAGACTCAGACACTAGAGAGTTTGGAATTCATTCCACAGCATGTT
GGTGCCTTCTCTGTGGAGAAATATGATGACATCAGGAAATATTTAATAAAGGCCTGTGACACTCCTCTG
CACCCCCTGGGCAGGCTTGTGGAGACACTGGTTGCAGTGTATAGAATGACATACGTGGGCGTAGGAGCC
AACCGCAGGTTATTGCAGGAGGCTGTAAAGGAGATTAAGTCCTATCTTAAGCGAATTTTCCAGCTGGTG
AGGTTCTTATTTCCTGAGCTGCCTGAAGAAGGCAGCACAATTCCTCTCTCTGCTCCTCTGCCAACCGAA
AGGAAGTCTTTTTGCACTGGGAAGTCAGATTCCCGATCTGAATCACCAGAGCCAGGTTATGTAGTAACG
AGTTCTGGATTATTGCTTCCTGTGCTGCTACCTCGGCTCTACCCACCGCTGTTTATGCTTTATGCTTTG
GATAATGATCGCGAGGAAGACATTTACTGGGAATGTGTCCTTCGACTAAATAAGCAGCCAGATATTGCT
CTCCTGGGCTTTCTTGGGGTGCAGAGGAAATTTTGGCCAGCAACCTTGTCAATCCTTGGAGAGAGTAAA
AAGGTTTTGCCAACCACGAAAGATGCTTGTTTTGCCTCAGCAGTAGAATGTCTGCAGCAGATCAGCACA
ACATTTACCCCATCAGACAAACTTAAGGTCATCCAGCAGACTTTTGAGGAGATCTCTCAGAGTGTCCTG
GCGTCACTCCACGAAGACTTCTTGTGGTCCATGGATGACTTGTTTCCTGTTTTCTTATATGTGGTGCTA
CGGGCCAGGATTAGGAATTTAGGCTCTGAGGTACACCTCATTGAGGATCTAATGGACCCCTATCTTCAG
CATGGGGAACAGGGTATAATGTTCACCACCTTGAAGGCATGTTACTACCAGATTCAGCGTGAGAAGCTT
AACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_020919
Insert Size 4974 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020919.2
RefSeq Size 6470 bp
RefSeq ORF 4974 bp
Locus ID 57679
UniProt ID Q96Q42
Domains RCC1, VPS9, MORN
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS)
MW 183.6 kDa
Gene Summary The protein encoded by this gene contains an ATS1/RCC1-like domain, a RhoGEF domain, and a vacuolar protein sorting 9 (VPS9) domain, all of which are guanine-nucleotide exchange factors that activate members of the Ras superfamily of GTPases. The protein functions as a guanine nucleotide exchange factor for the small GTPase RAB5. The protein localizes with RAB5 on early endosomal compartments, and functions as a modulator for endosomal dynamics. Mutations in this gene result in several forms of juvenile lateral sclerosis and infantile-onset ascending spastic paralysis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), also known as the LF or long form.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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