SPTBN1 (NM_003128) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	betaSpII; ELF; HEL102; SPTB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309012 representing NM_003128. Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGACGACCACAGTAGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGTGATGTCAACAAC CGCTGGGATGTCGACGACTGGGACAATGAGAACAGCTCTGCGCGGCTTTTTGAGCGGTCCCGCATCAAG GCTCTGGCAGATGAGCGTGAAGCCGTGCAGAAGAAGACCTTCACCAAGTGGGTCAATTCCCACCTTGCC CGTGTGTCCTGCCGGATCACAGACCTGTACACTGACCTTCGAGATGGACGGATGCTCATCAAGCTGCTG GAGGTCCTCTCTGGAGAGAGGCTGCCTAAACCCACCAAGGGACGAATGCGCATCCACTGCTTAGAGAAT GTGGACAAGGCCCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCTTGAGAACATGGGGTCCCATGACATC GTGGATGGAAACCACCGGCTGACCCTTGGCCTCATCTGGACCATCATCCTGCGCTTCCAGATCCAGGAT ATCAGTGTGGAAACTGAAGACAACAAAGAGAAGAAATCTGCCAAGGATGCATTGCTGTTGTGGTGCCAG ATGAAGACAGCTGGGTACCCCAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGACGGCATGGCC TTCAATGCACTGATACACAAACACCGGCCTGACCTGATAGATTTTGACAAACTAAAGAAATCTAACGCA CACTACAACCTGCAGAATGCATTTAATCTGGCAGAACAGCACCTCGGCCTCACTAAACTGTTGGACCCC GAAGACATCAGCGTGGACCATCCTGATGAGAAGTCCATAATCACTTATGTGGTGACTTATTACCACTAC TTCTCTAAGATGAAGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTGCTTGACAATGCTATTGAA ACAGAAAAAATGATTGAAAAGTATGAATCACTTGCCTCTGACCTTCTGGAATGGATTGAACAAACCATC ATCATTCTGAACAATCGCAAATTTGCCAATTCACTGGTCGGGGTTCAACAGCAGCTTCAGGCATTCAAC ACTTACCGCACTGTGGAGAAACCACCCAAATTTACTGAGAAGGGGAACTTGGAAGTGCTGCTCTTCACC ATTCAGAGCAAGATGAGGGCCAACAACCAGAAGGTCTACATGCCCCGGGAGGGGAAGCTCATCTCTGAC ATCAACAAGGCCTGGGAAAGACTGGAAAAAGCGGAACACGAAAGAGAACTGGCTTTGCGGAATGAGCTC ATAAGACAGGAGAAACTGGAACAGCTCGCCCGCAGATTTGATCGCAAGGCAGCTATGAGGGAGACTTGG CTGAGCGAAAACCAGCGTCTGGTGTCTCAGGACAACTTTGGGTTTGACCTTCCTGCAGTTGAGGCCGCC ACAAAAAAGCACGAGGCCATTGAGACAGACATTGCCGCATACGAGGAGCGTGTGCAGGCTGTGGTAGCC GTGGCCAGGGAGCTCGAGGCCGAGAATTACCACGACATCAAGCGCATCACAGCGAGGAAGGACAATGTC ATCCGGCTCTGGGAATACCTACTGGAACTGCTCAGGGCCCGGAGACAGCGGCTCGAGATGAACCTGGGG CTGCAGAAGATATTCCAGGAAATGCTCTACATTATGGACTGGATGGATGAAATGAAGGTGCTAGTATTG TCTCAAGACTATGGCAAACACTTACTTGGTGTGGAAGACCTGTTACAGAAGCACACCCTGGTTGAAGCA GACATTGGCATCCAGGCAGAGCGGGTGAGAGGTGTCAATGCCTCCGCCCAGAAGTTCGCAACAGACGGG GAAGGTTACAAGCCCTGTGACCCCCAGGTGATCCGAGACCGCGTGGCCCACATGGAGTTCTGTTATCAA GAGCTTTGCCAGCTGGCGGCTGAGCGCAGGGCCCGTCTGGAAGAGTCCCGCCGCCTCTGGAAGTTCTTC TGGGAGATGGCAGAAGAGGAAGGCTGGATACGGGAGAAGGAGAAGATCCTGTCCTCGGACGATTACGGG AAAGACCTGACCAGCGTCATGCGCCTGCTCAGCAAGCACCGGGCGTTCGAGGACGAGATGAGCGGCCGC AGTGGCCACTTTGAGCAGGCCATCAAGGAAGGCGAAGACATGATCGCGGAGGAGCACTTCGGGTCGGAG AAGATCCGTGAGAGGATCATTTACATCCGGGAGCAGTGGGCCAACCTAGAGCAGCTCTCGGCCATTCGG AAGAAGCGCCTGGAGGAGGCCTCCCTGCTGCACCAGTTCCAGGCAGATGCTGATGACATTGATGCCTGG ATGCTGGACATCCTCAAGATTGTCTCCAGCAGCGACGTGGGCCACGATGAGTATTCCACACAGTCTCTG GTCAAGAAACACAAGGACGTGGCGGAAGAGATCGCCAATTACAGGCCCACCCTTGACACGCTGCACGAA CAAGCCAGCGCCCTCCCCCAGGAGCATGCCGAGTCTCCAGACGTGAGGGGCAGGCTGTCGGGCATCGAG GAGCGGTATAAGGAGGTGGCAGAGCTGACGCGGCTGCGGAAGCAGGCACTCCAGGACACTCTGGCCCTG TACAAGATGTTCAGCGAGGCTGATGCCTGTGAGCTCTGGATCGACGAGAAGGAGCAGTGGCTCAACAAC ATGCAGATCCCAGAGAAGCTGGAGGATCTGGAGGTCATCCAGCACAGATTTGAGAGCCTAGAACCAGAA ATGAACAACCAGGCTTCCCGGGTTGCAGTGGTGAACCAGATTGCACGCCAGCTGATGCACAGCGGCCAC CCAAGTGAGAAGGAAATCAAAGCCCAGCAGGACAAACTCAACACAAGGTGGAGCCAGTTCAGAGAACTG GTTGACAGGAAGAAGGATGCCCTCCTGTCTGCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAA ACCAAATCCTGGATTCGGGAAAAGACCAAGGTCATCGAGTCCACCCAGGACCTGGGCAATGACCTGGCT GGCGTCATGGCCCTGCAGCGCAAGCTGACCGGCATGGAGCGGGACTTGGTGGCCATTGAGGCAAAGCTG AGTGACCTGCAGAAGGAGGCGGAGAAGCTGGAGTCCGAGCACCCCGACCAGGCCCAGGCCATCCTGTCT CGGCTGGCCGAGATCAGCGACGTGTGGGAGGAGATGAAGACCACCCTGAAAAACCGAGAGGCCTCCCTG GGAGAGGCCAGCAAGCTGCAGCAGTTCCTACGGGACTTGGACGACTTCCAGTCCTGGCTCTCTAGGACC CAGACAGCGATCGCCTCGGAGGACATGCCAAACACCCTGACCGAGGCTGAGAAGCTGCTCACGCAGCAC GAGAACATCAAGAACGAGATCGACAACTACGAGGAGGACTACCAGAAGATGAGGGACATGGGCGAGATG GTCACCCAGGGGCAGACCGATGCCCAGTACATGTTTCTGCGGCAGCGGCTGCAGGCCCTGGACACTGGA TGGAACGAGCTCCACAAGATGTGGGAGAACAGACAAAATCTCCTATCCCAGTCACATGCCTACCAGCAG TTCCTCAGAGACACGAAGCAAGCCGAAGCCTTTCTTAACAACCAGGAGTATGTTCTGGCTCACACTGAA ATGCCTACCACCTTGGAAGGAGCTGAAGCAGCAATTAAAAAGCAAGAGGACTTCATGACCACCATGGAC GCCAATGAGGAGAAGATCAATGCTGTGGTGGAGACTGGCCGGAGGCTGGTGAGCGATGGGAACATCAAC TCAGATCGCATCCAGGAGAAGGTGGACTCTATTGATGACAGACATAGGAAGAATCGTGAGACAGCCAGT GAACTTTTGATGAGGTTGAAGGACAACAGGGATCTACAGAAATTCCTGCAAGATTGTCAAGAGCTGTCT CTCTGGATCAATGAGAAGATGCTCACAGCCCAGGACATGTCTTACGATGAAGCCAGAAATCTGCACAGT AAATGGTTGAAGCATCAAGCATTTATGGCAGAACTTGCATCCAACAAAGAATGGCTTGACAAAATCGAG AAGGAAGGAATGCAGCTCATTTCAGAAAAGCCTGAGACGGAAGCTGTGGTGAAGGAGAAACTCACTGGT TTACATAAAATGTGGGAAGTCCTTGAATCCACTACCCAGACAAAGGCCCAGCGGCTCTTTGATGCAAAC AAGGCCGAACTTTTCACCCAGAGCTGTGCAGATCTAGACAAATGGCTGCACGGCCTGGAGAGTCAGATT CAGTCTGATGACTATGGCAAAGACCTGACCAGTGTCAATATCCTGCTGAAAAAGCAACAGATGCTGGAG AATCAGATGGAAGTGCGGAAGAAGGAGATCGAAGAGCTCCAAAGCCAAGCCCAGGCCCTGAGTCAGGAA GGGAAGAGCACCGACGAGGTAGACAGCAAGCGCCTCACCGTGCAGACCAAGTTCATGGAGTTGCTGGAG CCCTTGAACGAGAGGAAGCATAACCTGCTGGCCTCCAAAGAGATCCATCAGTTCAACAGGGATGTGGAG GACGAGATCTTGTGGGTTGGAGAGAGGATGCCTTTGGCAACTTCCACGGATCATGGCCACAACCTCCAG ACTGTGCAGCTGTTAATAAAGAAAAATCAGACCCTCCAGAAAGAAATCCAGGGGCACCAGCCTCGCATT GACGACATCTTTGAGAGGAGCCAAAACATCGTCACTGACAGCAGCAGCCTCAGCGCTGAGGCCATCAGA CAGAGGCTTGCCGACCTGAAGCAGCTGTGGGGTCTCCTCATTGAGGAGACAGAGAAACGCCACAGGCGG CTGGAGGAGGCGCACAGGGCCCAGCAGTACTACTTTGACGCTGCTGAGGCCGAAGCCTGGATGAGCGAG CAGGAGCTGTACATGATGTCAGAGGAGAAGGCCAAGGATGAGCAGAGTGCTGTCTCCATGTTGAAGAAG CACCAGATCTTAGAACAAGCTGTGGAGGACTATGCAGAGACCGTGCATCAGCTCTCCAAGACCAGCCGG GCCCTGGTGGCCGACAGCCATCCTGAAAGTGAGCGCATTAGCATGCGGCAGTCCAAAGTGGATAAACTG TACGCTGGTCTGAAAGACCTTGCTGAAGAGAGAAGAGGCAAGCTGGATGAGAGACACAGGTTATTCCAG CTCAACCGGGAGGTGGACGACCTGGAGCAGTGGATCGCTGAGAGGGAGGTGGTCGCAGGGTCCCATGAA CTGGGACAGGACTATGAGCATGTCACGATGTTACAAGAACGATTCCGGGAGTTTGCCCGAGACACCGGG AACATTGGGCAGGAGCGCGTGGACACGGTCAATCACCTGGCAGATGAGCTCATCAACTCTGGACATTCA GATGCCGCCACCATCGCTGAATGGAAGGATGGCCTCAATGAAGCCTGGGCCGACCTCCTGGAGCTCATT GACACAAGAACACAGATTCTTGCCGCTTCCTATGAACTGCACAAGTTTTACCACGATGCCAAGGAGATC TTTGGGCGTATACAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGGAGAGATCAGAACACAGTGGAG ACCTTACAGAGAATGCACACTACATTTGAGCATGACATCCAGGCTCTGGGCACACAGGTGAGGCAGCTG CAGGAGGATGCAGCCCGCCTCCAGGCGGCCTATGCGGGTGACAAGGCCGACGATATCCAGAAGCGCGAG AACGAGGTCCTGGAAGCCTGGAAGTCCCTCCTGGACGCCTGTGAGAGCCGCAGGGTGCGGCTGGTGGAC ACAGGGGACAAGTTCCGCTTCTTCAGCATGGTGCGCGACCTCATGCTCTGGATGGAGGATGTCATCCGG CAGATCGAGGCCCAGGAGAAGCCAAGGGATGTATCATCTGTTGAACTCTTAATGAATAATCATCAAGGC ATCAAAGCTGAAATTGATGCACGTAATGACAGTTTCACAACCTGCATTGAACTTGGGAAATCCCTGTTG GCGAGAAAACACTATGCATCTGAGGAGATCAAGGAAAAATTACTGCAGTTGACGGAAAAGAGGAAAGAA ATGATCGACAAGTGGGAAGACCGATGGGAATGGTTAAGACTGATTCTGGAGGTCCATCAGTTCTCAAGA GACGCCAGTGTGGCCGAGGCCTGGCTGCTTGGACAGGAGCCGTACCTATCCAGCCGAGAGATAGGCCAG AGCGTGGACGAGGTGGAGAAGCTCATCAAGCGCCACGAGGCATTTGAAAAGTCTGCAGCAACCTGGGAT GAGAGGTTCTCTGCCCTGGAAAGGCTGACTACATTGGAGTTACTGGAAGTGCGCAGACAGCAAGAGGAA GAGGAGAGGAAGAGGCGGCCGCCTTCTCCCGAGCCGAGCACGAAGGTTTCAGAGGAAGCCGAGTCCCAG CAGCAGTGGGATACTTCAAAAGGAGAACAAGTTTCCCAAAACGGTTTGCCAGCTGAACAGGGATCTCCA CGGATGGCAGAAACGGTGGACACAAGCGAAATGGTCAACGGCGCTACAGAACAAAGGACGAGCTCTAAA GAGTCCAGCCCCATCCCCTCCCCGACCTCTGATCGTAAAGCCAAGACTGCCCTCCCAGCCCAGAGTGCC GCCACCTTACCAGCCAGAACCCAGGAGACACCTTCGGCCCAGATGGAAGGCTTCCTCAATCGGAAACAC GAGTGGGAGGCCCACAATAAGAAAGCCTCAAGCAGGTCCTGGCACAATGTTTATTGTGTCATAAATAAC CAAGAAATGGGTTTCTACAAAGATGCAAAGACTGCTGCTTCTGGAATTCCCTACCACAGCGAGGTCCCT GTGAGTTTGAAAGAAGCTGTCTGCGAAGTGGCCCTTGATTACAAAAAGAAGAAACACGTATTCAAGCTA AGACTAAATGATGGCAATGAGTACCTCTTCCAAGCCAAAGACGATGAGGAAATGAACACATGGATCCAG GCTATCTCTTCCGCCATCTCCTCTGATAAACACGAGGTGTCTGCCAGCACCCAGAGCACGCCAGCATCC AGCCGCGCGCAGACCCTCCCCACCAGCGTCGTCACCATCACCAGCGAGTCCAGTCCCGGCAAGCGGGAA AAGGACAAAGAGAAAGACAAAGAGAAGCGGTTCAGCCTTTTTGGCAAAAAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	AscI-MluI
ACCN	NM_003128
Insert Size	7095 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003128.2
RefSeq Size	10238 bp
RefSeq ORF	7095 bp
Locus ID	6711
UniProt ID	Q01082
Domains	CH, PH, spectrin
MW	274.6 kDa
Gene Summary	Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1), also known as long or sigma-1, represents the longer transcript and encodes the longer isoform (1).

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